WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H326743
CAS#: 21343-40-8
Description: Ercalcidiol, also known as 25-Hydroxyvitamin D2 or 25-OH vitamin D2, is a a vitamin D2 metabolite.
Hodoodo Cat#: H326743
Name: Ercalcidiol
CAS#: 21343-40-8
Chemical Formula: C28H44O2
Exact Mass: 412.33
Molecular Weight: 412.658
Elemental Analysis: C, 81.50; H, 10.75; O, 7.75
Synonym: Ercalcidiol; 25-Hydroxyecalciferol; 25-Hydroxyergocalciferol; 25-Hydroxyvitamin D; 25-Hydroxyvitamin D2; 25-OH Vitamin D2.
IUPAC/Chemical Name: (R,Z)-3-(2-((1R,3aS,7aR,E)-1-((2R,5S,E)-6-hydroxy-5,6-dimethylhept-3-en-2-yl)-7a-methyloctahydro-4H-inden-4-ylidene)ethylidene)-4-methylenecyclohexan-1-ol
InChi Key: KJKIIUAXZGLUND-FOZBYKFWSA-N
InChi Code: InChI=1S/C28H44O2/c1-19-10-14-24(29)18-23(19)13-12-22-8-7-17-28(6)25(15-16-26(22)28)20(2)9-11-21(3)27(4,5)30/h9,11-13,20-21,24-26,29-30H,1,7-8,10,14-18H2,2-6H3/b11-9+,22-12+,23-13-/t20-,21+,24-,25-,26+,28-/m1/s1
SMILES Code: C[C@H](C(C)(O)C)/C=C/[C@@H](C)[C@H]([C@]1(CCC/2)C)CC[C@@]1([H])C2=C\C=C3C[C@H](O)CCC\3=C
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO
Shelf Life: >2 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info:
| Biological target: | |
| In vitro activity: | |
| In vivo activity: |
The following data is based on the product molecular weight 412.66 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
| Formulation protocol: | |
| In vitro protocol: | |
| In vivo protocol: |
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12: Williams DM, Fraser A, Sayers A, Fraser WD, Hyppönen E, Smith GD, Sattar N, Lawlor DA. Associations of childhood 25-hydroxyvitamin D2 and D3 and cardiovascular risk factors in adolescence: prospective findings from the Avon Longitudinal Study of Parents and Children. Eur J Prev Cardiol. 2014 Mar;21(3):281-90. doi: 10.1177/2047487312465688. Epub 2012 Nov 26. PubMed PMID: 23185083; PubMed Central PMCID: PMC3931583.
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18: Schleicher RL, Encisco SE, Chaudhary-Webb M, Paliakov E, McCoy LF, Pfeiffer CM. Isotope dilution ultra performance liquid chromatography-tandem mass spectrometry method for simultaneous measurement of 25-hydroxyvitamin D2, 25-hydroxyvitamin D3 and 3-epi-25-hydroxyvitamin D3 in human serum. Clin Chim Acta. 2011 Aug 17;412(17-18):1594-9. doi: 10.1016/j.cca.2011.05.010. Epub 2011 May 13. PubMed PMID: 21601563.
19: Stepman HC, Vanderroost A, Van Uytfanghe K, Thienpont LM. Candidate reference measurement procedures for serum 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 by using isotope-dilution liquid chromatography-tandem mass spectrometry. Clin Chem. 2011 Mar;57(3):441-8. doi: 10.1373/clinchem.2010.152553. Epub 2011 Jan 19. PubMed PMID: 21248072.
20: Netzel BC, Cradic KW, Bro ET, Girtman AB, Cyr RC, Singh RJ, Grebe SK. Increasing liquid chromatography-tandem mass spectrometry throughput by mass tagging: a sample-multiplexed high-throughput assay for 25-hydroxyvitamin D2 and D3. Clin Chem. 2011 Mar;57(3):431-40. doi: 10.1373/clinchem.2010.157115. Epub 2011 Jan 18. PubMed PMID: 21245371.
