Y39983 dihydrochlortide
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Hodoodo CAT#: H526789

CAS#: 173897-44-4 (2HCl)

Description: Y-39983, also known as Y-33075, is a potent and selective inhibitor of ROCK family with IC50 value of 3.6nM for ROCK. Y-39983 attenuates experimental autoimmune encephalomyelitis via inhibition of demyelination. Y-39983 downregulates RhoA/Rho-associated kinase expression during its promotion of axonal regeneration. Y-39983, promotes regeneration of crushed axons of retinal ganglion cells into the optic nerve of adult cats.


Chemical Structure

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Y39983 dihydrochlortide
CAS# 173897-44-4 (2HCl)

Theoretical Analysis

Hodoodo Cat#: H526789
Name: Y39983 dihydrochlortide
CAS#: 173897-44-4 (2HCl)
Chemical Formula: C16H18Cl2N4O
Exact Mass: 0.00
Molecular Weight: 353.247
Elemental Analysis: C, 54.40; H, 5.14; Cl, 20.07; N, 15.86; O, 4.53

Price and Availability

Size Price Availability Quantity
10mg USD 350 2 weeks
25mg USD 750 2 Weeks
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Related CAS #: 173897-44-4 (2HCl)   199433-58-4 (free base)   471843-75-1 (HCl)    

Synonym: Y-39983 HCl; Y-39983 hydrochloride; Y-39983 dihydrochloride; Y-39983; Y 39983; Y39983; Y-33075; Y 33075; Y33075.

IUPAC/Chemical Name: 4-[(1R)-1-aminoethyl]-N-(1H-pyrrolo[2,3-b]pyridin-4-yl)benzamide dihydrochloride

InChi Key: CKFHAVRPVZNMGT-YQFADDPSSA-N

InChi Code: InChI=1S/C16H16N4O.2ClH/c1-10(17)11-2-4-12(5-3-11)16(21)20-14-7-9-19-15-13(14)6-8-18-15;;/h2-10H,17H2,1H3,(H2,18,19,20,21);2*1H/t10-;;/m1../s1

SMILES Code: O=C(NC1=C2C(NC=C2)=NC=C1)C3=CC=C([C@H](N)C)C=C3.[H]Cl.[H]Cl

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 353.25 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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6: Van de Velde S, Van Bergen T, Sijnave D, Hollanders K, Castermans K, Defert O, Leysen D, Vandewalle E, Moons L, Stalmans I. AMA0076, a novel, locally acting Rho kinase inhibitor, potently lowers intraocular pressure in New Zealand white rabbits with minimal hyperemia. Invest Ophthalmol Vis Sci. 2014 Feb 18;55(2):1006-16. doi: 10.1167/iovs.13-13157. PMID: 24474276.


7: Okumura N, Nakano S, Kay EP, Numata R, Ota A, Sowa Y, Sakai T, Ueno M, Kinoshita S, Koizumi N. Involvement of cyclin D and p27 in cell proliferation mediated by ROCK inhibitors Y-27632 and Y-39983 during corneal endothelium wound healing. Invest Ophthalmol Vis Sci. 2014 Jan 15;55(1):318-29. doi: 10.1167/iovs.13-12225. PMID: 24106120.


8: Gao C, Huang L, Long Y, Zheng J, Yang J, Pu S, Xie L. Y-39983, a selective Rho-kinase inhibitor, attenuates experimental autoimmune encephalomyelitis via inhibition of demyelination. Neuroimmunomodulation. 2013;20(6):334-40. doi: 10.1159/000353568. Epub 2013 Aug 30. PMID: 24008512.


9: Yang Z, Wang J, Liu X, Cheng Y, Deng L, Zhong Y. Y-39983 downregulates RhoA/Rho-associated kinase expression during its promotion of axonal regeneration. Oncol Rep. 2013 Mar;29(3):1140-6. doi: 10.3892/or.2012.2205. Epub 2012 Dec 20. PMID: 23258382.


10: Tokushige H, Waki M, Takayama Y, Tanihara H. Effects of Y-39983, a selective Rho-associated protein kinase inhibitor, on blood flow in optic nerve head in rabbits and axonal regeneration of retinal ganglion cells in rats. Curr Eye Res. 2011 Oct;36(10):964-70. doi: 10.3109/02713683.2011.599106. PMID: 21950703.


11: Ramachandran C, Patil RV, Combrink K, Sharif NA, Srinivas SP. Rho-Rho kinase pathway in the actomyosin contraction and cell-matrix adhesion in immortalized human trabecular meshwork cells. Mol Vis. 2011;17:1877-90. Epub 2011 Jul 14. PMID: 21850162; PMCID: PMC3144732.


12: Watabe H, Abe S, Yoshitomi T. Effects of Rho-associated protein kinase inhibitors Y-27632 and Y-39983 on isolated rabbit ciliary arteries. Jpn J Ophthalmol. 2011 Jul;55(4):411-417. doi: 10.1007/s10384-011-0048-9. Epub 2011 Jun 11. PMID: 21667088.


13: Watanabe M. Regeneration of optic nerve fibers of adult mammals. Dev Growth Differ. 2010 Sep;52(7):567-76. doi: 10.1111/j.1440-169X.2010.01203.x. PMID: 20887558.


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15: Whitlock NA, Harrison B, Mixon T, Yu XQ, Wilson A, Gerhardt B, Eberhart DE, Abuin A, Rice DS. Decreased intraocular pressure in mice following either pharmacological or genetic inhibition of ROCK. J Ocul Pharmacol Ther. 2009 Jun;25(3):187-94. doi: 10.1089/jop.2008.0142. PMID: 19456252.


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18: Tokushige H, Inatani M, Nemoto S, Sakaki H, Katayama K, Uehata M, Tanihara H. Effects of topical administration of y-39983, a selective rho-associated protein kinase inhibitor, on ocular tissues in rabbits and monkeys. Invest Ophthalmol Vis Sci. 2007 Jul;48(7):3216-22. doi: 10.1167/iovs.05-1617. PMID: 17591891.


19: Sagawa H, Terasaki H, Nakamura M, Ichikawa M, Yata T, Tokita Y, Watanabe M. A novel ROCK inhibitor, Y-39983, promotes regeneration of crushed axons of retinal ganglion cells into the optic nerve of adult cats. Exp Neurol. 2007 May;205(1):230-40. doi: 10.1016/j.expneurol.2007.02.002. Epub 2007 Feb 14. PMID: 17359977.


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