HOE 33342 trihydrochlorde
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    WARNING: This product is for research use only, not for human or veterinary use.

Hodoodo CAT#: H530376

CAS#: 23491-52-3 (3HCl)

Description: HOE 33342, also known as Hoechst 33342, HO342, is a Benzimidazole fluorescent dye and a Cell permeable fluorescent DNA stain; binds minor groove of AT-rich regions. HOE 33342 trihydrochlorde is used to quantify DNA in viable cells.


Chemical Structure

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HOE 33342 trihydrochlorde
CAS# 23491-52-3 (3HCl)

Theoretical Analysis

Hodoodo Cat#: H530376
Name: HOE 33342 trihydrochlorde
CAS#: 23491-52-3 (3HCl)
Chemical Formula: C27H31Cl3N6O
Exact Mass: 0.00
Molecular Weight: 561.936
Elemental Analysis: C, 57.71; H, 5.56; Cl, 18.93; N, 14.96; O, 2.85

Price and Availability

Size Price Availability Quantity
100mg USD 450 2 Weeks
200mg USD 750 2 Weeks
500mg USD 1050 2 Weeks
1g USD 1850 2 Weeks
2g USD 2950 2 Weeks
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Synonym: HOE 33342 trihydrochlorde; Hoechst-33342; Hoechst33342; Hoechst 33342; HO342, NSC 334072.

IUPAC/Chemical Name: 2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole trihydrochloride

InChi Key: JABNPSKWVNCGMX-UHFFFAOYSA-N

InChi Code: InChI=1S/C27H28N6O.3ClH/c1-3-34-21-8-4-18(5-9-21)26-28-22-10-6-19(16-24(22)30-26)27-29-23-11-7-20(17-25(23)31-27)33-14-12-32(2)13-15-33;;;/h4-11,16-17H,3,12-15H2,1-2H3,(H,28,30)(H,29,31);3*1H

SMILES Code: CN1CCN(C2=CC=C3C(N=C(C4=CC=C5C(N=C(C6=CC=C(OCC)C=C6)N5)=C4)N3)=C2)CC1.[H]Cl.[H]Cl.[H]Cl

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 561.94 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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