WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H407432
CAS#: 1859141-26-6
Description: ROC-325 is an autophagy inhibitor. ROC-325 exhibited superior in vitro anticancer effects compared with the existing autophagy inhibitor hydroxychloroquine (HCQ) in 12 different cancer cell lines with diverse genetic backgrounds. ROC-325 antagonized RCC growth and survival in an ATG5/7-dependent manner, induced apoptosis, and exhibited favorable selectivity.
Hodoodo Cat#: H407432
Name: ROC-325
CAS#: 1859141-26-6
Chemical Formula: C28H27ClN4OS
Exact Mass: 502.16
Molecular Weight: 503.060
Elemental Analysis: C, 66.85; H, 5.41; Cl, 7.05; N, 11.14; O, 3.18; S, 6.37
Synonym: ROC-325; ROC 325; ROC325.
IUPAC/Chemical Name: 1-((2-((2-((7-chloroquinolin-4-yl)amino)ethyl)(methyl)amino)ethyl)amino)-4-methyl-9H-thioxanthen-9-one
InChi Key: HXUYKEGAEIYPKY-UHFFFAOYSA-N
InChi Code: InChI=1S/C28H27ClN4OS/c1-18-7-10-23(26-27(34)21-5-3-4-6-25(21)35-28(18)26)32-14-16-33(2)15-13-31-22-11-12-30-24-17-19(29)8-9-20(22)24/h3-12,17,32H,13-16H2,1-2H3,(H,30,31)
SMILES Code: O=C1C2=C(C(C)=CC=C2NCCN(C)CCNC3=C(C=CC(Cl)=C4)C4=NC=C3)SC5=CC=CC=C51
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO
Shelf Life: >2 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info:
Biological target: | |
In vitro activity: | |
In vivo activity: |
The following data is based on the product molecular weight 503.06 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
---|---|---|---|
1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
Formulation protocol: | |
In vitro protocol: | |
In vivo protocol: |
1: Carew JS, Nawrocki ST. Drain the lysosome: Development of the novel orally
available autophagy inhibitor ROC-325. Autophagy. 2017 Apr 3;13(4):765-766. doi:
10.1080/15548627.2017.1280222. Epub 2017 Jan 24. PubMed PMID: 28118053.
2: Carew JS, Espitia CM, Zhao W, Han Y, Visconte V, Phillips J, Nawrocki ST.
Disruption of Autophagic Degradation with ROC-325 Antagonizes Renal Cell
Carcinoma Pathogenesis. Clin Cancer Res. 2016 Nov 23. doi:
10.1158/1078-0432.CCR-16-1742. [Epub ahead of print] PubMed PMID: 27881580.