WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H532369
CAS#: 265646-85-3
Description: Endovion, also known as NS3728 and SCO‐101 is ANO1 inhibitor.
Hodoodo Cat#: H532369
Name: Endovion
CAS#: 265646-85-3
Chemical Formula: C16H9BrF6N6O
Exact Mass: 493.99
Molecular Weight: 495.183
Elemental Analysis: C, 38.81; H, 1.83; Br, 16.14; F, 23.02; N, 16.97; O, 3.23
Synonym: NS3728; NS 3728; NS-3728; Endovion; SCO‐101; SCO101; SCO 101; emidurdar
IUPAC/Chemical Name: 1-[3,5-bis(trifluoromethyl)phenyl]-3-[4-bromo-2-(1H-1,2,3,4-tetrazol-5-yl)phenyl]urea
InChi Key: AEFYFGMSRKDXHZ-UHFFFAOYSA-N
InChi Code: InChI=1S/C16H9BrF6N6O/c17-9-1-2-12(11(6-9)13-26-28-29-27-13)25-14(30)24-10-4-7(15(18,19)20)3-8(5-10)16(21,22)23/h1-6H,(H2,24,25,30)(H,26,27,28,29)
SMILES Code: O=C(NC1=CC=C(Br)C=C1C2=NN=NN2)NC3=CC(C(F)(F)F)=CC(C(F)(F)F)=C3
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO
Shelf Life: >2 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info:
| Biological target: | |
| In vitro activity: | |
| In vivo activity: |
The following data is based on the product molecular weight 495.18 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
| Formulation protocol: | |
| In vitro protocol: | |
| In vivo protocol: |
1: Godwin TD, Kelly ST, Brew TP, Bougen-Zhukov NM, Single AB, Chen A, Stylianou CE, Harris LD, Currie SK, Telford BJ, Beetham HG, Evans GB, Black MA, Guilford PJ. E-cadherin-deficient cells have synthetic lethal vulnerabilities in plasma membrane organisation, dynamics and function. Gastric Cancer. 2019 Mar;22(2):273-286. doi: 10.1007/s10120-018-0859-1. Epub 2018 Jul 31. PMID: 30066183; PMCID: PMC6394693.
2: Sørensen BH, Nielsen D, Thorsteinsdottir UA, Hoffmann EK, Lambert IH. Downregulation of LRRC8A protects human ovarian and alveolar carcinoma cells against Cisplatin-induced expression of p53, MDM2, p21Waf1/Cip1, and Caspase-9/-3 activation. Am J Physiol Cell Physiol. 2016 Jun 1;310(11):C857-73. doi: 10.1152/ajpcell.00256.2015. Epub 2016 Mar 16. PMID: 26984736; PMCID: PMC4935196.
3: Sauter DRP, Novak I, Pedersen SF, Larsen EH, Hoffmann EK. ANO1 (TMEM16A) in pancreatic ductal adenocarcinoma (PDAC). Pflugers Arch. 2015 Jul;467(7):1495-1508. doi: 10.1007/s00424-014-1598-8. Epub 2014 Aug 28. PMID: 25163766; PMCID: PMC4464647.
4: Poulsen KA, Andersen EC, Hansen CF, Klausen TK, Hougaard C, Lambert IH, Hoffmann EK. Deregulation of apoptotic volume decrease and ionic movements in multidrug-resistant tumor cells: role of chloride channels. Am J Physiol Cell Physiol. 2010 Jan;298(1):C14-25. doi: 10.1152/ajpcell.00654.2008. Epub 2009 Oct 21. PMID: 19846756.
5: Kjaer K, Strøbaek D, Christophersen P, Rønn LC. Chloride channel blockers inhibit iNOS expression and NO production in IFNgamma-stimulated microglial BV2 cells. Brain Res. 2009 Jul 24;1281:15-24. doi: 10.1016/j.brainres.2009.05.015. Epub 2009 May 13. Erratum in: Brain Res. 2009 Sep 29;1291:153. PMID: 19446535.
6: Schneider L, Klausen TK, Stock C, Mally S, Christensen ST, Pedersen SF, Hoffmann EK, Schwab A. H-ras transformation sensitizes volume-activated anion channels and increases migratory activity of NIH3T3 fibroblasts. Pflugers Arch. 2008 Mar;455(6):1055-62. doi: 10.1007/s00424-007-0367-3. Epub 2007 Oct 20. PMID: 17952454.
7: Klausen TK, Bergdahl A, Hougaard C, Christophersen P, Pedersen SF, Hoffmann EK. Cell cycle-dependent activity of the volume- and Ca2+-activated anion currents in Ehrlich lettre ascites cells. J Cell Physiol. 2007 Mar;210(3):831-42. doi: 10.1002/jcp.20918. PMID: 17111356.
8: Hélix N, Strøbaek D, Dahl BH, Christophersen P. Inhibition of the endogenous volume-regulated anion channel (VRAC) in HEK293 cells by acidic di-aryl-ureas. J Membr Biol. 2003 Nov 15;196(2):83-94. doi: 10.1007/s00232-003-0627-x. PMID: 14724745.
