WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H531000
CAS#: 915-67-3
Description: Amaranth Dye, also known as E-123, is an azo dye classified as an endocrine disruptor. It was observed that the lower the pH the higher the degradation. In addition, an enhancement in the photodegradation rate was observed by the addition of hydrogen peroxide as an electron acceptor. The adsorption trends of Amaranth at various initial concentrations followed the Langmuir isotherm trend.
Hodoodo Cat#: H531000
Name: Amaranth Dye (80%)
CAS#: 915-67-3
Chemical Formula: C20H11N2Na3O10S3
Exact Mass: 603.93
Molecular Weight: 604.461
Elemental Analysis: C, 39.74; H, 1.83; N, 4.63; Na, 11.41; O, 26.47; S, 15.91
Synonym: Amaranth Dye, Azo, E-123, E123, E 123
IUPAC/Chemical Name: sodium (E)-3-hydroxy-4-((4-sulfonatonaphthalen-1-yl)diazenyl)naphthalene-2,7-disulfonate
InChi Key: WLDHEUZGFKACJH-ZRUFZDNISA-K
InChi Code: InChI=1S/C20H14N2O10S3.3Na/c23-20-18(35(30,31)32)10-11-9-12(33(24,25)26)5-6-13(11)19(20)22-21-16-7-8-17(34(27,28)29)15-4-2-1-3-14(15)16;;;/h1-10,23H,(H,24,25,26)(H,27,28,29)(H,30,31,32);;;/q;3*+1/p-3/b22-21+;;;
SMILES Code: O=S(C1=C(O)C(/N=N/C2=C3C=CC=CC3=C(S(=O)([O-])=O)C=C2)=C4C=CC(S(=O)([O-])=O)=CC4=C1)([O-])=O.[Na+].[Na+].[Na+]
Appearance: Dark red to purple solid powder.
Purity: >80% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO
Shelf Life: >2 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info:
| Biological target: | Amaranth is a dark red to purple azo dye used as a food dye and to color cosmetics. |
| In vitro activity: | Fig. 2a shows inhibition of proliferation vs soluble protein concentration curves, where antiproliferative effects of amaranth proteins and peptides on HT-29 tumor cells are exhibited. DGS presented the highest inhibition of proliferation with an IC50value of 0.30 ± 0.07 mg soluble protein/mL, while API showed a moderate effect with an IC50 value of 1.35 ± 0.12 mg soluble protein/mL. HT-29 cells were susceptible to DGS and API treatments, with significantly lower values IC50 of DGS (p < 0.05). Reference: Plant Foods Hum Nutr. 2019 Mar;74(1):107-114. https://pubmed.ncbi.nlm.nih.gov/30635822/ |
| In vivo activity: | At the family level, the abundance of Bifidobacteriaceae tended to be higher in the Ama (Amaranth) group than in HF group (p = 0.055, Figure 6A). The commensal bacteria including unclassified S24-7, Ruminococcaceae, unclassified Clostridiales, Lachnospiraceae, and unclassified RF-39 were significantly enriched in the Ama group compared to those in the HF group (Figure 6B–F). The abundance of Porphyromonadaceae and Peptostreptococcaceae (Figure 6G,H) was lower in the Ama group than in the HF group. Collectively, these results showed that Ama supplementation modulated the gut microbiota of HF diet-fed mice, resulting in a microbiota composition similar to that in Con mice. Reference: Foods. 2021 Jun; 10(6): 1259. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8229566/ |
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 62.5 | 103.40 | |
| Water | 6.7 | 11.03 |
The following data is based on the product molecular weight 604.46 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
| Formulation protocol: | 1. Schröter D, Neugart S, Schreiner M, Grune T, Rohn S, Ott C. Amaranth's 2-Caffeoylisocitric Acid-An Anti-Inflammatory Caffeic Acid Derivative That Impairs NF-κB Signaling in LPS-Challenged RAW 264.7 Macrophages. Nutrients. 2019 Mar 7;11(3):571. doi: 10.3390/nu11030571. PMID: 30866427; PMCID: PMC6471825. 2. Sabbione AC, Ogutu FO, Scilingo A, Zhang M, Añón MC, Mu TH. Antiproliferative Effect of Amaranth Proteins and Peptides on HT-29 Human Colon Tumor Cell Line. Plant Foods Hum Nutr. 2019 Mar;74(1):107-114. doi: 10.1007/s11130-018-0708-8. PMID: 30635822. 3. Yang Y, Fukui R, Jia H, Kato H. Amaranth Supplementation Improves Hepatic Lipid Dysmetabolism and Modulates Gut Microbiota in Mice Fed a High-Fat Diet. Foods. 2021 Jun 1;10(6):1259. doi: 10.3390/foods10061259. PMID: 34206088; PMCID: PMC8229566. 4. Suárez S, Aphalo P, Rinaldi G, Añón MC, Quiroga A. Effect of amaranth proteins on the RAS system. In vitro, in vivo and ex vivo assays. Food Chem. 2020 Mar 5;308:125601. doi: 10.1016/j.foodchem.2019.125601. Epub 2019 Oct 4. PMID: 31670190. |
| In vitro protocol: | 1. Schröter D, Neugart S, Schreiner M, Grune T, Rohn S, Ott C. Amaranth's 2-Caffeoylisocitric Acid-An Anti-Inflammatory Caffeic Acid Derivative That Impairs NF-κB Signaling in LPS-Challenged RAW 264.7 Macrophages. Nutrients. 2019 Mar 7;11(3):571. doi: 10.3390/nu11030571. PMID: 30866427; PMCID: PMC6471825. 2. Sabbione AC, Ogutu FO, Scilingo A, Zhang M, Añón MC, Mu TH. Antiproliferative Effect of Amaranth Proteins and Peptides on HT-29 Human Colon Tumor Cell Line. Plant Foods Hum Nutr. 2019 Mar;74(1):107-114. doi: 10.1007/s11130-018-0708-8. PMID: 30635822. |
| In vivo protocol: | 1. Yang Y, Fukui R, Jia H, Kato H. Amaranth Supplementation Improves Hepatic Lipid Dysmetabolism and Modulates Gut Microbiota in Mice Fed a High-Fat Diet. Foods. 2021 Jun 1;10(6):1259. doi: 10.3390/foods10061259. PMID: 34206088; PMCID: PMC8229566. 2. Suárez S, Aphalo P, Rinaldi G, Añón MC, Quiroga A. Effect of amaranth proteins on the RAS system. In vitro, in vivo and ex vivo assays. Food Chem. 2020 Mar 5;308:125601. doi: 10.1016/j.foodchem.2019.125601. Epub 2019 Oct 4. PMID: 31670190. |
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