WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H407823
CAS#: 431979-47-4
Description: SJ-172550, also known as MDMX Inhibitor II, is an inhibitor of MDMX that disrupts MDMX-p53 peptide interaction. It forms a covalent, but reversible, cysteine adduct in the MDMX p53-binding domain, locking MDMX into a conformation that prevents binding to p53.
Hodoodo Cat#: H407823
Name: SJ-172550
CAS#: 431979-47-4
Chemical Formula: C22H21ClN2O5
Exact Mass: 428.11
Molecular Weight: 428.870
Elemental Analysis: C, 61.61; H, 4.94; Cl, 8.27; N, 6.53; O, 18.65
Synonym: SJ-172550; SJ 172550; SJ172550. MDMX Inhibitor II.
IUPAC/Chemical Name: methyl (Z)-2-(2-chloro-6-ethoxy-4-((3-methyl-5-oxo-1-phenyl-1,5-dihydro-4H-pyrazol-4-ylidene)methyl)phenoxy)acetate
InChi Key: RKKFQJXGAQWHBZ-YVLHZVERSA-N
InChi Code: InChI=1S/C22H21ClN2O5/c1-4-29-19-12-15(11-18(23)21(19)30-13-20(26)28-3)10-17-14(2)24-25(22(17)27)16-8-6-5-7-9-16/h5-12H,4,13H2,1-3H3/b17-10-
SMILES Code: ClC1=CC(/C=C2C(N(C3=CC=CC=C3)N=C\2C)=O)=CC(OCC)=C1OCC(OC)=O
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO
Shelf Life: >2 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info:
Biological target: | |
In vitro activity: | |
In vivo activity: |
The following data is based on the product molecular weight 428.87 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
---|---|---|---|
1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
Formulation protocol: | |
In vitro protocol: | |
In vivo protocol: |
1: Bista M, Smithson D, Pecak A, Salinas G, Pustelny K, Min J, Pirog A, Finch K, Zdzalik M, Waddell B, Wladyka B, Kedracka-Krok S, Dyer MA, Dubin G, Guy RK. On the mechanism of action of SJ-172550 in inhibiting the interaction of MDM4 and p53. PLoS One. 2012;7(6):e37518. doi: 10.1371/journal.pone.0037518. Epub 2012 Jun 4. PubMed PMID: 22675482; PubMed Central PMCID: PMC3366986.
2: Leão M, Gomes S, Bessa C, Soares J, Raimundo L, Monti P, Fronza G, Pereira C, Saraiva L. Studying p53 family proteins in yeast: induction of autophagic cell death and modulation by interactors and small molecules. Exp Cell Res. 2015 Jan 1;330(1):164-77. doi: 10.1016/j.yexcr.2014.09.028. Epub 2014 Sep 28. PubMed PMID: 25265062.
3: Lemos A, Leão M, Soares J, Palmeira A, Pinto M, Saraiva L, Sousa ME. Medicinal Chemistry Strategies to Disrupt the p53-MDM2/MDMX Interaction. Med Res Rev. 2016 Sep;36(5):789-844. doi: 10.1002/med.21393. Epub 2016 Jun 15. Review. PubMed PMID: 27302609.
4: Noushini S, Alipour E, Emami S, Safavi M, Ardestani SK, Gohari AR, Shafiee A, Foroumadi A. Synthesis and cytotoxic properties of novel (E)-3-benzylidene-7-methoxychroman-4-one derivatives. Daru. 2013 Apr 12;21(1):31. doi: 10.1186/2008-2231-21-31. PubMed PMID: 23587260; PubMed Central PMCID: PMC3668990.
5: Reed D, Shen Y, Shelat AA, Arnold LA, Ferreira AM, Zhu F, Mills N, Smithson DC, Regni CA, Bashford D, Cicero SA, Schulman BA, Jochemsen AG, Guy RK, Dyer MA. Identification and characterization of the first small molecule inhibitor of MDMX. J Biol Chem. 2010 Apr 2;285(14):10786-96. doi: 10.1074/jbc.M109.056747. Epub 2010 Jan 15. PubMed PMID: 20080970; PubMed Central PMCID: PMC2856285.
6: Leão M, Gomes S, Soares J, Bessa C, Maciel C, Ciribilli Y, Pereira C, Inga A, Saraiva L. Novel simplified yeast-based assays of regulators of p53-MDMX interaction and p53 transcriptional activity. FEBS J. 2013 Dec;280(24):6498-507. doi: 10.1111/febs.12552. Epub 2013 Oct 23. PubMed PMID: 24119020.
7: Soares J, Raimundo L, Pereira NA, dos Santos DJ, Pérez M, Queiroz G, Leão M, Santos MM, Saraiva L. A tryptophanol-derived oxazolopiperidone lactam is cytotoxic against tumors via inhibition of p53 interaction with murine double minute proteins. Pharmacol Res. 2015 May-Jun;95-96:42-52. doi: 10.1016/j.phrs.2015.03.006. Epub 2015 Mar 23. PubMed PMID: 25814188.