Y27632 free base
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Hodoodo CAT#: H341079

CAS#: 146986-50-7 (free base)

Description: Y27632 is a selective ROCK inhibitor, which inhibits ET-1-induced increases in natriuretic peptide production, cell size, protein synthesis, and myofibrillar organization. Y27632 prevents dimethylnitrosamine-induced hepatic fibrosis in rats, increases apoptosis and disrupts the actin cortical mat in embryonic avian corneal epithelium, affects initial heart myofibrillogenesis in cultured chick blastoderm, promotes the proliferation and cell cycle progression of cultured astrocyte from spinal cord.


Chemical Structure

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Y27632 free base
CAS# 146986-50-7 (free base)

Theoretical Analysis

Hodoodo Cat#: H341079
Name: Y27632 free base
CAS#: 146986-50-7 (free base)
Chemical Formula: C14H21N3O
Exact Mass: 247.17
Molecular Weight: 247.340
Elemental Analysis: C, 67.98; H, 8.56; N, 16.99; O, 6.47

Price and Availability

Size Price Availability Quantity
100mg USD 950 2 Weeks
5mg USD 1250 2 Weeks
200mg USD 1650 2 Weeks
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Related CAS #: 331752-47-7 (HCl hydrate)   129830-38-2 (2HCl)   146986-50-7 (free base)   138381-45-0 (racemate HCl)   310898-86-3 (recamte free base)    

Synonym: Y 27632; Y-27632; Y27632.

IUPAC/Chemical Name: (1R,4r)-4-((R)-1-aminoethyl)-N-(pyridin-4-yl)cyclohexanecarboxamide

InChi Key: IYOZTVGMEWJPKR-IJLUTSLNSA-N

InChi Code: InChI=1S/C14H21N3O/c1-10(15)11-2-4-12(5-3-11)14(18)17-13-6-8-16-9-7-13/h6-12H,2-5,15H2,1H3,(H,16,17,18)/t10-,11-,12-/m1/s1

SMILES Code: O=C([C@H]1CC[C@H]([C@H](N)C)CC1)NC2=CC=NC=C2

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 247.34 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Qian H, Leng X, Wen J, Zhou Q, Xu X, Wu X. One-Step Simple Isolation Method to Obtain Both Epidermal and Dermal Stem Cells from Human Skin Specimen. Methods Mol Biol. 2018 Feb 25. doi: 10.1007/7651_2018_117. [Epub ahead of print] PubMed PMID: 29478135.

2: Park JT, Kang HT, Park CH, Lee YS, Cho KA, Park SC. A crucial role of ROCK for alleviation of senescence-associated phenotype. Exp Gerontol. 2018 Feb 20. pii: S0531-5565(17)30645-9. doi: 10.1016/j.exger.2018.02.012. [Epub ahead of print] PubMed PMID: 29474864.

3: Dolinina J, Rippe A, Bentzer P, Öberg CM. Glomerular hyperpermeability after acute unilateral ureteral obstruction: Effects of Tempol, NOS-, RhoA- and Rac-1-inhibition. Am J Physiol Renal Physiol. 2018 Feb 21. doi: 10.1152/ajprenal.00610.2017. [Epub ahead of print] PubMed PMID: 29465305.

4: Sahın L, Cevık OS, Koyuncu DD, Buyukafsar K. Role of rho-kinase (ROCK) in tonic but not phasic contraction in the frog stomach smooth muscle. Life Sci. 2018 Feb 15. pii: S0024-3205(18)30073-0. doi: 10.1016/j.lfs.2018.02.023. [Epub ahead of print] PubMed PMID: 29455004.

5: Zhuang RJ, Jin WD, Wang XY, Wu XM. Identification and characterization of the druggable kinase targets of olmesartan and its analogues from a systematic kinase-chemical interaction profile in atherosclerosis. J Mol Graph Model. 2018 Mar;80:211-216. doi: 10.1016/j.jmgm.2018.01.016. PubMed PMID: 29414040.

6: Mochalov SV, Tarasova NV, Kudryashova TV, Gaynullina DK, Kalenchuk VU, Borovik AS, Vorotnikov AV, Tarasova OS, Schubert R. Higher Ca(2+) -sensitivity of arterial contraction in 1-week-old rats is due to a greater Rho-kinase activity. Acta Physiol (Oxf). 2018 Jan 31. doi: 10.1111/apha.13044. [Epub ahead of print] PubMed PMID: 29383848.

7: Guo X, Wei S, Lu M, Shao Z, Lu J, Xia L, Lin K, Zou D. RNA-Seq investigation and in vivo study the effect of strontium ranelate on ovariectomized rat via the involvement of ROCK1. Artif Cells Nanomed Biotechnol. 2018 Jan 30:1-13. doi: 10.1080/21691401.2018.1433188. [Epub ahead of print] PubMed PMID: 29381089.

8: Lee BH, Choi SH, Kim HJ, Park SD, Rhim H, Kim HC, Hwang SH, Nah SY. Gintonin absorption in intestinal model systems. J Ginseng Res. 2018 Jan;42(1):35-41. doi: 10.1016/j.jgr.2016.12.007. Epub 2016 Dec 19. PubMed PMID: 29348720; PubMed Central PMCID: PMC5766688.

9: Liang H, Liao M, Zhao W, Zheng X, Xu F, Wang H, Huang J. CXCL16/ROCK1 signaling pathway exacerbates acute kidney injury induced by ischemia-reperfusion. Biomed Pharmacother. 2018 Feb;98:347-356. doi: 10.1016/j.biopha.2017.12.063. Epub 2017 Dec 27. PubMed PMID: 29275176.

10: Domokos D, Fülöp F, Falkay G, Gáspár R. Effects of newly synthetized isoquinoline derivatives on rat uterine contractility and ROCK II activity. Bioorg Med Chem Lett. 2017 Dec 13. pii: S0960-894X(17)31177-0. doi: 10.1016/j.bmcl.2017.12.017. [Epub ahead of print] PubMed PMID: 29269216.

11: Yu Y, Min F, Ye H, Fang T, Jia X, Zhou C. [Y-27632 protects H9c2 cardiomyocytes against hypoxia/reoxygenation injury by inhibiting autophagy pathway]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2017 Nov;33(11):1511-1515. Chinese. PubMed PMID: 29268855.

12: Feng ZH, Zhang XH, Zhao JQ, Ma JZ. Involvement of Rho-associated coiled-coil kinase signaling inhibition in TGF-β1/Smad2, 3 signal transduction in vitro. Int J Ophthalmol. 2017 Dec 18;10(12):1805-1811. doi: 10.18240/ijo.2017.12.03. eCollection 2017. PubMed PMID: 29259896; PubMed Central PMCID: PMC5733505.

13: McCulley DJ, Wienhold MD, Hines EA, Hacker TA, Rogers A, Pewowaruk RJ, Zewdu R, Chesler NC, Selleri L, Sun X. PBX transcription factors drive pulmonary vascular adaptation to birth. J Clin Invest. 2018 Feb 1;128(2):655-667. doi: 10.1172/JCI93395. Epub 2017 Dec 18. PubMed PMID: 29251627; PubMed Central PMCID: PMC5785269.

14: Muñoz VR, Gaspar RC, Kuga GK, da Rocha AL, Crisol BM, Botezelli JD, Baptista IL, Mekary RA, da Silva ASR, Cintra DE, de Moura LP, Ropelle ER, Pauli JR. Exercise increases Rho-kinase activity and insulin signaling in skeletal muscle. J Cell Physiol. 2018 Jun;233(6):4791-4800. doi: 10.1002/jcp.26278. Epub 2018 Jan 15. PubMed PMID: 29219181.

15: Blasio A, Wang J, Wang D, Varodayan FP, Pomrenze MB, Miller J, Lee AM, McMahon T, Gyawali S, Wang HY, Roberto M, McHardy S, Pleiss MA, Messing RO. Novel Small-Molecule Inhibitors of Protein Kinase C Epsilon Reduce Ethanol Consumption in Mice. Biol Psychiatry. 2017 Dec 1. pii: S0006-3223(17)32127-3. doi: 10.1016/j.biopsych.2017.10.017. [Epub ahead of print] PubMed PMID: 29198469.

16: Nuhn JAM, Perez AM, Schneider IC. Contact guidance diversity in rotationally aligned collagen matrices. Acta Biomater. 2018 Jan 15;66:248-257. doi: 10.1016/j.actbio.2017.11.039. Epub 2017 Nov 28. PubMed PMID: 29196116; PubMed Central PMCID: PMC5750117.

17: Alarcon VB, Marikawa Y. ROCK and RHO Playlist for Preimplantation Development: Streaming to HIPPO Pathway and Apicobasal Polarity in the First Cell Differentiation. Adv Anat Embryol Cell Biol. 2018;229:47-68. doi: 10.1007/978-3-319-63187-5_5. PubMed PMID: 29177764.

18: Zhou N, Lee JJ, Stoll S, Ma B, Costa KD, Qiu H. Rho Kinase Regulates Aortic Vascular Smooth Muscle Cell Stiffness Via Actin/SRF/Myocardin in Hypertension. Cell Physiol Biochem. 2017;44(2):701-715. doi: 10.1159/000485284. Epub 2017 Nov 23. PubMed PMID: 29169155.

19: Zou Y, Ma L, Zhao Y, Zhang S, Zhou C, Cai Y. Inhibition of Rho kinase protects against colitis in mice by attenuating intestinal epithelial barrier dysfunction via MLC and the NF-κB pathway. Int J Mol Med. 2018 Jan;41(1):430-438. doi: 10.3892/ijmm.2017.3197. Epub 2017 Oct 19. PubMed PMID: 29115372.

20: Roy Chowdhury U, Rinkoski TA, Bahler CK, Millar JC, Bertrand JA, Holman BH, Sherwood JM, Overby DR, Stoltz KL, Dosa PI, Fautsch MP. Effect of Cromakalim Prodrug 1 (CKLP1) on Aqueous Humor Dynamics and Feasibility of Combination Therapy With Existing Ocular Hypotensive Agents. Invest Ophthalmol Vis Sci. 2017 Nov 1;58(13):5731-5742. doi: 10.1167/iovs.17-22538. PubMed PMID: 29114841; PubMed Central PMCID: PMC5678549.