Eriochrome Black T

    WARNING: This product is for research use only, not for human or veterinary use.

Hodoodo CAT#: H341181

CAS#: 1787-61-7

Description: Eriochrome Black T is a complexometric indicator that is used in complexometric titrations, e.g.

Chemical Structure

img
Eriochrome Black T
CAS# 1787-61-7
Instruction

Theoretical Analysis

Hodoodo Cat#: H341181
Name: Eriochrome Black T
CAS#: 1787-61-7
Chemical Formula: C20H12N3NaO7S
Exact Mass: 461.03
Molecular Weight: 461.380
Elemental Analysis: C, 52.07; H, 2.62; N, 9.11; Na, 4.98; O, 24.27; S, 6.95

Price and Availability

This product is not in stock, which may be available by custom synthesis. For cost-effective reason, minimum order is 1g (price is usually high, lead time is 2~3 months, depending on the technical challenge). Quote less than 1g will not be provided. To request quote, please email to sales @hodoodo.com or click below button.
Note: Price will be listed if it is available in the future.

Request quote for custom synthesis

Synonym: Eriochrome Black T; NSC 7223; NSC-7223; NSC7223.

IUPAC/Chemical Name: 1-Naphthalenesulfonic acid, 3-hydroxy-4-((1-hydroxy-2-naphthalenyl)azo)-7-nitro-, monosodium salt

InChi Key: AMMWFYKTZVIRFN-QUABFQRHSA-M

InChi Code: InChI=1S/C20H13N3O7S.Na/c24-17-10-18(31(28,29)30)15-9-12(23(26)27)6-7-14(15)19(17)22-21-16-8-5-11-3-1-2-4-13(11)20(16)25;/h1-10,24-25H,(H,28,29,30);/q;+1/p-1/b22-21+;

SMILES Code: c12c(c(c(O)cc1S(=O)(=O)[O-])\N=N\c1c(c3c(cccc3)cc1)O)ccc(c2)[N+](=O)[O-].[Na+]

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 461.38 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

1: Jarujamrus P, Malahom N, Puchum S, Meelapsom R, Amatatongchai M, Siripinyanond A, Chairam S, Kulsing C. Complexometric and argentometric titrations using thread-based analytical devices. Talanta. 2018 Jun 1;183:228-236. doi: 10.1016/j.talanta.2018.02.058. Epub 2018 Feb 17. PubMed PMID: 29567169.

2: Wang SN, Chen QJ, Zhu MJ, Xue FY, Li WC, Zhao TJ, Li GD, Zhang GQ. An extracellular yellow laccase from white rot fungus Trametes sp. F1635 and its mediator systems for dye decolorization. Biochimie. 2018 Feb 28. pii: S0300-9084(18)30058-0. doi: 10.1016/j.biochi.2018.02.015. [Epub ahead of print] PubMed PMID: 29501482.

3: Yilmaz MD, Oktem HA. Eriochrome Black T-Eu(3+) Complex as a Ratiometric Colorimetric and Fluorescent Probe for the Detection of Dipicolinic Acid, a Biomarker of Bacterial Spores. Anal Chem. 2018 Mar 20;90(6):4221-4225. doi: 10.1021/acs.analchem.8b00576. Epub 2018 Mar 7. PubMed PMID: 29488375.

4: Karimi MH, Mahdavinia GR, Massoumi B, Baghban A, Saraei M. Ionically crosslinked magnetic chitosan/κ-carrageenan bioadsorbents for removal of anionic eriochrome black-T. Int J Biol Macromol. 2018 Feb 19;113:361-375. doi: 10.1016/j.ijbiomac.2018.02.102. [Epub ahead of print] PubMed PMID: 29471096.

5: Etchebarne BE, Li Z, Stedtfeld RD, Nicholas MC, Williams MR, Johnson TA, Stedtfeld TM, Kostic T, Khalife WT, Tiedje JM, Hashsham SA, Hughes MJ. Evaluation of Nucleic Acid Isothermal Amplification Methods for Human Clinical Microbial Infection Detection. Front Microbiol. 2017 Dec 12;8:2211. doi: 10.3389/fmicb.2017.02211. eCollection 2017. PubMed PMID: 29312154; PubMed Central PMCID: PMC5732957.

6: Srivastava A, Kumari U, Nigam AK, Mittal S, Mittal AK. Alterations in the activity of certain enzymes in the gills of a carp Labeo rohita exposed to an azo dye, Eriochrome black T: a biochemical investigation. Fish Physiol Biochem. 2018 Apr;44(2):629-637. doi: 10.1007/s10695-017-0458-9. Epub 2017 Dec 22. PubMed PMID: 29270704.

7: Chaudhary S, Sharma J, Kaith BS, Yadav S, Sharma AK, Goel A. Gum xanthan-psyllium-cl-poly(acrylic acid-co-itaconic acid) based adsorbent for effective removal of cationic and anionic dyes: Adsorption isotherms, kinetics and thermodynamic studies. Ecotoxicol Environ Saf. 2018 Mar;149:150-158. doi: 10.1016/j.ecoenv.2017.11.030. Epub 2017 Nov 20. PubMed PMID: 29156307.

8: Gholami A, Golestaneh M, Andalib Z. A new method for determination of cocamidopropyl betaine synthesized from coconut oil through spectral shift of Eriochrome Black T. Spectrochim Acta A Mol Biomol Spectrosc. 2018 Mar 5;192:122-127. doi: 10.1016/j.saa.2017.11.007. Epub 2017 Nov 10. PubMed PMID: 29128745.

9: Mudassir MA, Hussain SZ, Rehman A, Zaheer W, Asma ST, Jilani A, Aslam M, Zhang H, Ansari TM, Hussain I. Development of Silver-Nanoparticle-Decorated Emulsion-Templated Hierarchically Porous Poly(1-vinylimidazole) Beads for Water Treatment. ACS Appl Mater Interfaces. 2017 Jul 19;9(28):24190-24197. doi: 10.1021/acsami.7b05311. Epub 2017 Jul 3. PubMed PMID: 28644011.

10: Khashaba PY, Ali HRH, El-Wekil MM. Simultaneous voltammetric analysis of anti-ulcer and D(2)-antagonist agents in binary mixture using redox sensor and their determination in human serum. Mater Sci Eng C Mater Biol Appl. 2017 Jun 1;75:733-741. doi: 10.1016/j.msec.2017.02.092. Epub 2017 Feb 24. PubMed PMID: 28415523.

11: Zinatloo-Ajabshir S, Morassaei MS, Salavati-Niasari M. Facile fabrication of Dy(2)Sn(2)O(7)-SnO(2) nanocomposites as an effective photocatalyst for degradation and removal of organic contaminants. J Colloid Interface Sci. 2017 Jul 1;497:298-308. doi: 10.1016/j.jcis.2017.03.031. Epub 2017 Mar 6. PubMed PMID: 28288376.

12: Srivastava A, Verma N, Mistri A, Ranjan B, Nigam AK, Kumari U, Mittal S, Mittal AK. Alterations in the skin of Labeo rohita exposed to an azo dye, Eriochrome black T: a histopathological and enzyme biochemical investigation. Environ Sci Pollut Res Int. 2017 Mar;24(9):8671-8681. doi: 10.1007/s11356-017-8517-4. Epub 2017 Feb 15. PubMed PMID: 28197946.

13: Ning YJ, Wang SS, Chen QJ, Ling ZR, Wang SN, Wang WP, Zhang GQ, Zhu MJ. An extracellular yellow laccase with potent dye decolorizing ability from the fungus Leucoagaricus naucinus LAC-04. Int J Biol Macromol. 2016 Dec;93(Pt A):837-842. doi: 10.1016/j.ijbiomac.2016.09.046. Epub 2016 Sep 15. PubMed PMID: 27642125.

14: Thomas SP, Sankar HK. A simple colorimetric method for estimation of tramadol hydrochloride in pure and tablet dosage forms. Indian J Pharmacol. 2016 Sep-Oct;48(5):550-554. PubMed PMID: 27721542; PubMed Central PMCID: PMC5051250.

15: Nalbach M, Klassen S, Bechstein R, Kühnle A. Molecular Self-Assembly Versus Surface Restructuring During Calcite Dissolution. Langmuir. 2016 Oct 4;32(39):9975-9981. Epub 2016 Sep 21. PubMed PMID: 27603323.

16: Srivastava B, Barman MK, Chatterjee M, Roy D, Mandal B. Solid phase extraction, separation and preconcentration of rare elements thorium(IV), uranium(VI), zirconium(IV), cerium(IV) and chromium(III) amid several other foreign ions with eriochrome black T anchored to 3-D networking silica gel. J Chromatogr A. 2016 Jun 17;1451:1-14. doi: 10.1016/j.chroma.2016.04.079. Epub 2016 May 7. PubMed PMID: 27185054.

17: Molla A, Sahu M, Hussain S. Synthesis of Tunable Band Gap Semiconductor Nickel Sulphide Nanoparticles: Rapid and Round the Clock Degradation of Organic Dyes. Sci Rep. 2016 May 17;6:26034. doi: 10.1038/srep26034. PubMed PMID: 27185051; PubMed Central PMCID: PMC4868986.

18: Karita S, Kaneta T. Chelate titrations of Ca(2+) and Mg(2+) using microfluidic paper-based analytical devices. Anal Chim Acta. 2016 Jun 14;924:60-67. doi: 10.1016/j.aca.2016.04.019. Epub 2016 Apr 22. PubMed PMID: 27181645.

19: Busi S, Chatterjee R, Rajkumari J, Hnamte S. Ecofriendly biosorption of dyes and metals by bacterial biomass of Aeromonas hydrophila RC1. J Environ Biol. 2016 Mar;37(2):267-74. PubMed PMID: 27097447.

20: Jassal V, Shanker U, Kaith BS. Aegle marmelos Mediated Green Synthesis of Different Nanostructured Metal Hexacyanoferrates: Activity against Photodegradation of Harmful Organic Dyes. Scientifica (Cairo). 2016;2016:2715026. doi: 10.1155/2016/2715026. Epub 2016 Feb 29. PubMed PMID: 27034896; PubMed Central PMCID: PMC4789527.