Alizarine Yellow R
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    WARNING: This product is for research use only, not for human or veterinary use.

Hodoodo CAT#: H341183

CAS#: 2243-76-7

Description: Alizarine Yellow R is a yellow colored azo dye made by the diazo coupling reaction.


Chemical Structure

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Alizarine Yellow R
CAS# 2243-76-7

Theoretical Analysis

Hodoodo Cat#: H341183
Name: Alizarine Yellow R
CAS#: 2243-76-7
Chemical Formula: C13H9N3O5
Exact Mass: 287.05
Molecular Weight: 287.231
Elemental Analysis: C, 54.36; H, 3.16; N, 14.63; O, 27.85

Price and Availability

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1g USD 295
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Synonym: Alizarine Yellow R; C.I. 14030.

IUPAC/Chemical Name: 5-(p-Nitrophenylazo)salicylic acid

InChi Key: YVJPMMYYRNHJAU-CCEZHUSRSA-N

InChi Code: InChI=1S/C13H9N3O5/c17-12-6-3-9(7-11(12)13(18)19)15-14-8-1-4-10(5-2-8)16(20)21/h1-7,17H,(H,18,19)/b15-14+

SMILES Code: O=C(O)c1c(O)ccc(\N=N\c2ccc([N+]([O-])=O)cc2)c1

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 287.23 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Zhenqian Z, Sihler S, Ziener U. Alizarin Yellow R (AYR) as compatible stabilizer for miniemulsion polymerization. J Colloid Interface Sci. 2017 Dec 1;507:337-343. doi: 10.1016/j.jcis.2017.08.007. Epub 2017 Aug 3. PubMed PMID: 28803027.

2: Cui MH, Cui D, Gao L, Cheng HY, Wang AJ. Efficient azo dye decolorization in a continuous stirred tank reactor (CSTR) with built-in bioelectrochemical system. Bioresour Technol. 2016 Oct;218:1307-11. doi: 10.1016/j.biortech.2016.07.135. Epub 2016 Jul 30. PubMed PMID: 27497830.

3: Cui MH, Cui D, Lee HS, Liang B, Wang AJ, Cheng HY. Effect of electrode position on azo dye removal in an up-flow hybrid anaerobic digestion reactor with built-in bioelectrochemical system. Sci Rep. 2016 Apr 28;6:25223. doi: 10.1038/srep25223. PubMed PMID: 27121278; PubMed Central PMCID: PMC4848485.

4: Sun Q, Li ZL, Wang YZ, Yang CX, Chung JS, Wang AJ. Cathodic bacterial community structure applying the different co-substrates for reductive decolorization of Alizarin Yellow R. Bioresour Technol. 2016 May;208:64-72. doi: 10.1016/j.biortech.2016.02.003. Epub 2016 Feb 6. PubMed PMID: 26922314.

5: Sun Q, Li Z, Wang Y, Cui D, Liang B, Thangavel S, Chung JS, Wang A. A horizontal plug-flow baffled bioelectrocatalyzed reactor for the reductive decolorization of Alizarin Yellow R. Bioresour Technol. 2015 Nov;195:73-7. doi: 10.1016/j.biortech.2015.06.086. Epub 2015 Jun 25. PubMed PMID: 26142821.

6: Cui D, Guo YQ, Lee HS, Wu WM, Liang B, Wang AJ, Cheng HY. Enhanced decolorization of azo dye in a small pilot-scale anaerobic baffled reactor coupled with biocatalyzed electrolysis system (ABR-BES): a design suitable for scaling-up. Bioresour Technol. 2014 Jul;163:254-61. doi: 10.1016/j.biortech.2014.03.165. Epub 2014 Apr 13. PubMed PMID: 24821204.

7: Zolgharnein J, Asanjrani N, Bagtash M, Azimi G. Multi-response optimization using Taguchi design and principle component analysis for removing binary mixture of alizarin red and alizarin yellow from aqueous solution by nano γ-alumina. Spectrochim Acta A Mol Biomol Spectrosc. 2014 May 21;126:291-300. doi: 10.1016/j.saa.2014.01.100. Epub 2014 Feb 15. PubMed PMID: 24608013.

8: Cui D, Guo YQ, Cheng HY, Liang B, Kong FY, Lee HS, Wang AJ. Azo dye removal in a membrane-free up-flow biocatalyzed electrolysis reactor coupled with an aerobic bio-contact oxidation reactor. J Hazard Mater. 2012 Nov 15;239-240:257-64. doi: 10.1016/j.jhazmat.2012.08.072. Epub 2012 Sep 4. PubMed PMID: 23009797.

9: Liang B, Yao Q, Cheng H, Gao S, Kong F, Cui D, Guo Y, Ren N, Lee DJ, Wang A. Enhanced degradation of azo dye alizarin yellow R in a combined process of iron-carbon microelectrolysis and aerobic bio-contact oxidation. Environ Sci Pollut Res Int. 2012 Jun;19(5):1385-91. doi: 10.1007/s11356-012-0785-4. Epub 2012 Jun 20. Erratum in: Environ Sci Pollut Res Int. 2013 Feb;20(2):1206. Lee, Duu-Jong [added]. PubMed PMID: 22743988.

10: Shiri S, Delpisheh A, Haeri A, Poornajaf A, Golzadeh B, Shiri S. Determination of Trace Amounts of Lead Using the Flotation-spectrophotometric method. Anal Chem Insights. 2010 Dec 20;5:47-52. doi: 10.4137/ACI.S5948. Erratum in: Anal Chem Insights. 2011;6:45. PubMed PMID: 21234287; PubMed Central PMCID: PMC3018889.

11: Ihara K, Hasegawa S, Naito K. The separation of aluminum(III) ions from the aqueous solution on membrane filter using Alizarin Yellow R. Talanta. 2008 May 30;75(4):944-9. doi: 10.1016/j.talanta.2007.12.047. Epub 2008 Jan 14. PubMed PMID: 18585167.

12: Zhang H, Wang XM, Wang QC, Shi RM, Ding LS. [Fluorescence study on the interaction of alizarin yellow R and bovine serum albumin in alkali solution]. Guang Pu Xue Yu Guang Pu Fen Xi. 2006 Aug;26(8):1508-12. Chinese. PubMed PMID: 17058958.

13: Tang HA, Wang XF, Lei XY, Yang RD. [Studies on the preparation, characterization and fluorescence property of rare Earth with alizarin yellow R]. Guang Pu Xue Yu Guang Pu Fen Xi. 2004 Aug;24(8):975-7. Chinese. PubMed PMID: 15766123.

14: Bhuyan R, Saikia CN. Isolation of colour components from native dye-bearing plants in northeastern India. Bioresour Technol. 2005 Feb;96(3):363-72. PubMed PMID: 15474939.

15: Shama SA, Amin AS. Spectrophotometric microdetermination of nefopam, mebevrine and phenylpropanolamine hydrochloride in pharmaceutical formulations using alizarins. Spectrochim Acta A Mol Biomol Spectrosc. 2004 Jul;60(8-9):1769-74. PubMed PMID: 15248949.

16: Amin AS. Spectrophotometric determination of piroxicam and tenoxicam in pharmaceutical formulations using alizarin. J Pharm Biomed Anal. 2002 Jul 20;29(4):729-36. PubMed PMID: 12093502.

17: Zhou YC, Zheng RL. Phenolic compounds and an analog as superoxide anion scavengers and antioxidants. Biochem Pharmacol. 1991 Aug 22;42(6):1177-9. PubMed PMID: 1653565.

18: Brown JP, Roehm GW, Brown RJ. Mutagenicity testing of certified food colors and related azo, xanthene and triphenylmethane dyes with the Salmonella/microsome system. Mutat Res. 1978 Jan;56(3):249-71. PubMed PMID: 342943.