Diazobenzenesulfonic acid

    WARNING: This product is for research use only, not for human or veterinary use.

Hodoodo CAT#: H581155

CAS#: 2154-66-7

Description: Diazobenzenesulfonic acid is a whiter or reddish crystaline acid derivative of sulfanilic acid that is used as the reagent in the diazo teaction. Diazobenzenesulfonic acid is also known as diazobenzene sulfonic acid was reported to be a desensitizer (inhibitor) of aliostericeffect for myosin ATPase 13.


Chemical Structure

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Diazobenzenesulfonic acid
CAS# 2154-66-7

Theoretical Analysis

Hodoodo Cat#: H581155
Name: Diazobenzenesulfonic acid
CAS#: 2154-66-7
Chemical Formula: C6H5N2O3S
Exact Mass: 185.00
Molecular Weight: 185.180
Elemental Analysis: C, 38.92; H, 2.72; N, 15.13; O, 25.92; S, 17.31

Price and Availability

This product is not in stock, which may be available by custom synthesis. For cost-effective reason, minimum order is 1g (price is usually high, lead time is 2~3 months, depending on the technical challenge). Quote less than 1g will not be provided. To request quote, please email to sales @hodoodo.com or click below button.
Note: Price will be listed if it is available in the future.

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Synonym: Diazobenzenesulfonic acid; DASA; Diazobenzene sulfonate; Diazobenzenesulfonate; Diazobenzenesulphonate; diazobenzene sulfonic acid;

IUPAC/Chemical Name: 4-Sulfobenzenediazonium

InChi Key: UEUIKXVPXLWUDU-UHFFFAOYSA-O

InChi Code: InChI=1S/C6H4N2O3S/c7-8-5-1-3-6(4-2-5)12(9,10)11/h1-4H/p+1

SMILES Code: N#[N+]C1=CC=C(S(=O)(O)=O)C=C1

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 185.18 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: FRANCA F. [Effects of substances activating the diazo reaction and of diazobenzenesulfonic acid (diazoreactive) on natural bonds of bilirubin in organic fluids]. Acta Med Patav. 1955 Jan-Mar;15(1):69-76. Italian. PubMed PMID: 13394143.

2: Maksay G, Ticku MK. Characterization of gamma-aminobutyric acid-benzodiazepine receptor complexes by protection against inactivation by group-specific reagents. J Neurochem. 1984 Jun;42(6):1715-27. PubMed PMID: 6327911.

3: Kasai H, Takahashi K, Ando T. Chemical modification of tyrosine residues in ribonuclease T1 with N-acetylimidazole and p-diazobenzenesulfonic acid. J Biochem. 1977 Jun;81(6):1751-8. PubMed PMID: 408339.

4: STEINITZ K. [A simplified determination of serum bilirubin with diazobenzenesulfonic acid]. Harefuah. 1952 Jul 15;43(1-2):13. Undetermined Language. PubMed PMID: 13021645.

5: Nishimoto SK, Zhao J, Dass C. Isolation and characterization of the reaction product of 4-diazobenzenesulfonic acid and gamma-carboxyglutamic acid: modification of the assay for measurement of beta-carboxyaspartic acid. Anal Biochem. 1994 Jan;216(1):159-64. PubMed PMID: 8135347.

6: Landis JB, Pardue HL. Kinetics of the reactions of unconjugated and conjugated bilirubins with p-diazobenzenesulfonic acid. Clin Chem. 1978 Oct;24(10):1690-9. PubMed PMID: 29720.

7: Pfleiderer G, Jeckel D. [Coupling reaction of lactate dehydrogenase after reversible masking of the SH-groups. Reaction with p-diazobenzenesulfonic acid for marking essential histidine or tyrosine residues]. Eur J Biochem. 1967 Sep;2(2):171-5. German. PubMed PMID: 6078529.

8: Maksay G, Ticku MK. Diazotization and thiocyanate differentiate agonists from antagonists for the high- and low-affinity receptors of gamma-aminobutyric acid. J Neurochem. 1984 Jul;43(1):261-8. PubMed PMID: 6327917.

9: Nishimoto SK. A colorimetric assay specific for gamma-carboxyglutamic acid-containing proteins: its utility in protein purification procedures. Anal Biochem. 1990 May 1;186(2):273-9. PubMed PMID: 2363499.

10: Jie KS, Gijsbers BL, Vermeer C. A specific colorimetric staining method for gamma-carboxyglutamic acid-containing proteins in polyacrylamide gels. Anal Biochem. 1995 Jan 1;224(1):163-5. PubMed PMID: 7535983.

11: Kabasawa I, Murayama K, Kimura M, Sekine T. Thiols of myosin. II. Chemical modification of sulfhydryl groups of myosin A adenosine triphosphatase with diazobenzenesulfonic acid. J Biochem. 1974 Jun;75(6):1275-81. PubMed PMID: 4279252.

12: Artman M, Seeley RJ. Studies on the sidedness of the human red blood cell membrane: preparation of stable azo-erythrocytes. Prep Biochem. 1981;11(3):291-8. PubMed PMID: 6265896.

13: Shimada Y, Shinmyo A, Enatsu T. Purification and properties of one component of acid phosphatase produced by Aspergillus niger. Biochim Biophys Acta. 1977 Feb 9;480(2):417-27. PubMed PMID: 13843.

14: Bouchet MJ, Jacques P, Ilien B, Goeldner M, Hirth C. m-Sulfonate benzene diazonium chloride: a powerful affinity label for the gamma-aminobutyric acid binding site from rat brain. J Neurochem. 1992 Oct;59(4):1405-13. PubMed PMID: 1402891.

15: Burch TP, Thyagarajan R, Ticku MK. Group-selective reagent modification of the benzodiazepine-gamma-aminobutyric acid receptor-ionophore complex reveals that low-affinity gamma-aminobutyric acid receptors stimulate benzodiazepine binding. Mol Pharmacol. 1983 Jan;23(1):52-9. PubMed PMID: 6306426.

16: Goldstein IM, Cerqueira M, Lind S, Kaplan HB. Evidence that the superoxide-generating system of human leukocytes is associated with the cell surface. J Clin Invest. 1977 Feb;59(2):249-54. PubMed PMID: 188867; PubMed Central PMCID: PMC333354.

17: Ohno Y, Hirai K, Kanoh T, Uchino H, Ogawa K. Subcellular localization of H2O2 production in human neutrophils stimulated with particles and an effect of cytochalasin-B on the cells. Blood. 1982 Jul;60(1):253-60. PubMed PMID: 7082841.

18: BERKA A, ZYKA J. [Copulation reaction with p-diazobenzenesulphonic acid. I. Photometric determination of some drugs]. Cesk Farm. 1955 Jun;4(5):225-7. Czech. PubMed PMID: 14390578.

19: JINDRA A, KALVODA R, ZYKA J. [Polarometric determination of certain pharmaceutically important substances with para-diazobenzenesulphonic acid]. Cas Cesk Lek. 1950;63(7-8):106-10. Undetermined Language. PubMed PMID: 14792571.

20: McPhail LC, Henson PM, Johnston RB Jr. Respiratory burst enzyme in human neutrophils. Evidence for multiple mechanisms of activation. J Clin Invest. 1981 Mar;67(3):710-6. PubMed PMID: 6259208; PubMed Central PMCID: PMC370621.