Flu-IA
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    WARNING: This product is for research use only, not for human or veterinary use.

Hodoodo CAT#: H562394

CAS#: 63368-54-7

Description: Flu-IA is a fluorescent probe that covalently labels DNA fragments.


Chemical Structure

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Flu-IA
CAS# 63368-54-7

Theoretical Analysis

Hodoodo Cat#: H562394
Name: Flu-IA
CAS#: 63368-54-7
Chemical Formula: C26H24IN3O4
Exact Mass: 569.08
Molecular Weight: 569.390
Elemental Analysis: C, 54.84; H, 4.25; I, 22.29; N, 7.38; O, 11.24

Price and Availability

Size Price Availability Quantity
25mg USD 300 2 Weeks
50mg USD 550 2 Weeks
100mg USD 900 2 Weeks
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Synonym: Flu-IA; Flu IA; FluIA; 5-IAF; 5 IAF; 5IAF; 5-Iodoacetamidofluorescein; 5 Iodoacetamidofluorescein; 5Iodoacetamidofluorescein;

IUPAC/Chemical Name: N-(3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthen]-5-yl)-2-iodoacetamide

InChi Key: UATCLPJEZJKNHE-UHFFFAOYSA-N

InChi Code: InChI=1S/C22H14INO6/c23-10-20(27)24-11-1-4-15-14(7-11)21(28)30-22(15)16-5-2-12(25)8-18(16)29-19-9-13(26)3-6-17(19)22/h1-9,25-26H,10H2,(H,24,27)

SMILES Code: ICC(NC1=CC(C(OC2(C3=CC=C(O)C=C3O4)C5=C4C=C(O)C=C5)=O)=C2C=C1)=O

Appearance: Solid powder

Purity: >90% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 569.39 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Barrera C, López S, Aguilar L, Mercado L, Bravo M, Quiroz W. Pentavalent antimony uptake pathway through erythrocyte membranes: molecular and atomic fluorescence approaches. Anal Bioanal Chem. 2016 Apr;408(11):2937-44. doi: 10.1007/s00216-015-9188-y. Epub 2015 Nov 19. PubMed PMID: 26586161.

2: Indo HP, Matsui H, Chen J, Zhu H, Hawkins CL, Davies MJ, Yarana C, St Clair DK, Majima HJ. Manganese superoxide dismutase promotes interaction of actin, S100A4 and Talin, and enhances rat gastric tumor cell invasion. J Clin Biochem Nutr. 2015 Jul;57(1):13-20. doi: 10.3164/jcbn.14-146. Epub 2015 May 22. PubMed PMID: 26236095; PubMed Central PMCID: PMC4512892.

3: Williams E, Pead S, Whiteman M, Wood ME, Wilson ID, Ladomery MR, Teklic T, Lisjak M, Hancock JT. Detection of thiol modifications by hydrogen sulfide. Methods Enzymol. 2015;555:233-51. doi: 10.1016/bs.mie.2014.11.026. Epub 2015 Jan 8. PubMed PMID: 25747483.

4: Landino LM, Hagedorn TD, Kennett KL. Evidence for thiol/disulfide exchange reactions between tubulin and glyceraldehyde-3-phosphate dehydrogenase. Cytoskeleton (Hoboken). 2014 Dec;71(12):707-18. doi: 10.1002/cm.21204. Epub 2015 Jan 31. PubMed PMID: 25545749.

5: Rysev NA, Nevzorov IA, Avrova SV, Karpicheva OE, Redwood CS, Levitsky DI, Borovikov YS. Gly126Arg substitution causes anomalous behaviour of α-skeletal and β-smooth tropomyosins during the ATPase cycle. Arch Biochem Biophys. 2014 Feb 1;543:57-66. doi: 10.1016/j.abb.2013.12.016. Epub 2013 Dec 25. PubMed PMID: 24374033.

6: Clark HM, Hagedorn TD, Landino LM. Hypothiocyanous acid oxidation of tubulin cysteines inhibits microtubule polymerization. Arch Biochem Biophys. 2014 Jan 1;541:67-73. doi: 10.1016/j.abb.2013.10.026. Epub 2013 Nov 9. PubMed PMID: 24215946; PubMed Central PMCID: PMC3885989.

7: Misheva M, Kaur G, Ngoei KR, Yeap YY, Ng IH, Wagstaff KM, Ng DC, Jans DA, Bogoyevitch MA. Intracellular mobility and nuclear trafficking of the stress-activated kinase JNK1 are impeded by hyperosmotic stress. Biochim Biophys Acta. 2014 Feb;1843(2):253-64. doi: 10.1016/j.bbamcr.2013.10.017. Epub 2013 Nov 1. PubMed PMID: 24184208.

8: Lloyd MM, Grima MA, Rayner BS, Hadfield KA, Davies MJ, Hawkins CL. Comparative reactivity of the myeloperoxidase-derived oxidants hypochlorous acid and hypothiocyanous acid with human coronary artery endothelial cells. Free Radic Biol Med. 2013 Dec;65:1352-62. doi: 10.1016/j.freeradbiomed.2013.10.007. Epub 2013 Oct 10. PubMed PMID: 24120969.

9: Sotgia S, Pisanu E, Pintus G, Erre GL, Pinna GA, Deiana L, Carru C, Zinellu A. Plasma L-ergothioneine measurement by high-performance liquid chromatography and capillary electrophoresis after a pre-column derivatization with 5-iodoacetamidofluorescein (5-IAF) and fluorescence detection. PLoS One. 2013 Jul 29;8(7):e70374. doi: 10.1371/journal.pone.0070374. Print 2013. PubMed PMID: 23922985; PubMed Central PMCID: PMC3726632.

10: Borovikov IuS, Karpicheva OE, Rysev NA, Redwood CS. [Abnormal tropomyosin function in ATPase cycle in hypertrophic and dilated cardiomyopathies]. Ross Fiziol Zh Im I M Sechenova. 2013 Jan;99(1):73-80. Russian. PubMed PMID: 23659058.

11: Lopez-Davila AJ, Elhamine F, Ruess DF, Papadopoulos S, Iorga B, Kulozik FP, Zittrich S, Solzin J, Pfitzer G, Stehle R. Kinetic mechanism of Ca²⁺-controlled changes of skeletal troponin I in psoas myofibrils. Biophys J. 2012 Sep 19;103(6):1254-64. doi: 10.1016/j.bpj.2012.08.022. PubMed PMID: 22995498; PubMed Central PMCID: PMC3446660.

12: Liu J, Liu Y, Gao M, Zhang X. An accurate proteomic quantification method: fluorescence labeling absolute quantification (FLAQ) using multidimensional liquid chromatography and tandem mass spectrometry. Proteomics. 2012 Aug;12(14):2258-70. doi: 10.1002/pmic.201100649. PubMed PMID: 22887945.

13: Summers FA, Forsman Quigley A, Hawkins CL. Identification of proteins susceptible to thiol oxidation in endothelial cells exposed to hypochlorous acid and N-chloramines. Biochem Biophys Res Commun. 2012 Aug 24;425(2):157-61. doi: 10.1016/j.bbrc.2012.07.057. Epub 2012 Jul 20. PubMed PMID: 22819842.

14: Garrido-Medina R, Puerta A, Rivera-Monroy Z, de Frutos M, Guttman A, Diez-Masa JC. Analysis of alpha-1-acid glycoprotein isoforms using CE-LIF with fluorescent thiol derivatization. Electrophoresis. 2012 Apr;33(7):1113-9. doi: 10.1002/elps.201100473. PubMed PMID: 22539314.

15: Wang H, Wang S, Lu Y, Alvarez S, Hicks LM, Ge X, Xia Y. Proteomic analysis of early-responsive redox-sensitive proteins in Arabidopsis. J Proteome Res. 2012 Jan 1;11(1):412-24. doi: 10.1021/pr200918f. Epub 2011 Nov 21. PubMed PMID: 22050424; PubMed Central PMCID: PMC3253204.

16: Davis C, Gordon N, Murphy S, Singh I, Kavanagh K, Carberry S, Doyle S. Single-pot derivatisation strategy for enhanced gliotoxin detection by HPLC and MALDI-ToF mass spectrometry. Anal Bioanal Chem. 2011 Nov;401(8):2519-29. doi: 10.1007/s00216-011-5344-1. Epub 2011 Aug 31. PubMed PMID: 21879298.

17: Landino LM, Hagedorn TD, Kim SB, Hogan KM. Inhibition of tubulin polymerization by hypochlorous acid and chloramines. Free Radic Biol Med. 2011 Apr 15;50(8):1000-8. doi: 10.1016/j.freeradbiomed.2011.01.018. Epub 2011 Jan 21. PubMed PMID: 21256958; PubMed Central PMCID: PMC3051002.

18: Zinellu A, Lepedda A Jr, Sotgia S, Zinellu E, Marongiu G, Usai MF, Gaspa L, De Muro P, Formato M, Deiana L, Carru C. Albumin-bound low molecular weight thiols analysis in plasma and carotid plaques by CE. J Sep Sci. 2010 Jan;33(1):126-31. doi: 10.1002/jssc.200900582. PubMed PMID: 19950356.

19: Obata F, Kitami M, Inoue Y, Atsumi S, Yoshizawa Y, Sato R. Analysis of the region for receptor binding and triggering of oligomerization on Bacillus thuringiensis Cry1Aa toxin. FEBS J. 2009 Oct;276(20):5949-59. doi: 10.1111/j.1742-4658.2009.07275.x. Epub 2009 Sep 9. PubMed PMID: 19740106.

20: Chumsae C, Gaza-Bulseco G, Liu H. Identification and localization of unpaired cysteine residues in monoclonal antibodies by fluorescence labeling and mass spectrometry. Anal Chem. 2009 Aug 1;81(15):6449-57. doi: 10.1021/ac900815z. PubMed PMID: 19572546.