WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H571606
CAS#: 115616-51-8
Description: PDPH Crosslinker, or SPDP Hydrazide, is a cleavable, heterobifunctional protein crosslinker. PDPH crosslinking reagent's hydrazide group selectively reacts with oxidized carbohydrates (or carboxyls when used with EDC); while the pyridinyldisulfide moity is reactive toward reduced thiols (sulfhydryls; -SH). Thus, PDPH linker is an excellent choice for crosslinking glycoproteins and other polysaccharides.
Hodoodo Cat#: H571606
Name: PDPH Crosslinker
CAS#: 115616-51-8
Chemical Formula: C8H11N3OS2
Exact Mass: 229.03
Molecular Weight: 229.320
Elemental Analysis: C, 41.90; H, 4.84; N, 18.32; O, 6.98; S, 27.96
Synonym: PDPH Crosslinker, PDPH Cross-linker, PDPH Cross linker, SPDP Hydrazide
IUPAC/Chemical Name: 3-(2-Pyridyldithio)propionyl hydrazide
InChi Key: NITXODYAMWZEJY-UHFFFAOYSA-N
InChi Code: InChI=1S/C8H11N3OS2/c9-11-7(12)4-6-13-14-8-3-1-2-5-10-8/h1-3,5H,4,6,9H2,(H,11,12)
SMILES Code: O=C(NN)CCSSC1=NC=CC=C1
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO
Shelf Life: >2 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info: Additionally, PDPH crosslinking reagent's pyridyldisulfide group contains a cleavable disulfide (-S-S-) bond which exposes a thiol moity that can be further reacted with, for example, a maleimide containing crosslinker.
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The following data is based on the product molecular weight 229.32 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
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1: Ansell SM, Tardi PG, Buchkowsky SS. 3-(2-pyridyldithio)propionic acid hydrazide as a cross-linker in the formation of liposome-antibody conjugates. Bioconjug Chem. 1996 Jul-Aug;7(4):490-6. PubMed PMID: 8853463.
2: Suh W, Chung JK, Park SH, Kim SW. Anti-JL1 antibody-conjugated poly (L-lysine) for targeted gene delivery to leukemia T cells. J Control Release. 2001 May 14;72(1-3):171-8. PubMed PMID: 11389996.
