WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H571607
CAS#: 42014-51-7
Description: SBA crosslinker, or N-Succinimidyl bromoacetate, is a sulfhydryl (thiol) and amino reactive heterobifunctional protein crosslinking reagent. SBA protein crosslinker is non-cleavable and is among the shortest amine and sulfhydryl reactive crosslinking reagents known with a spacer arm length of only 1.5 Angstroms. SBA Crosslinker is useful for making antibody drug conjugates (ADCs).
Hodoodo Cat#: H571607
Name: SBA Crosslinker
CAS#: 42014-51-7
Chemical Formula: C6H6BrNO4
Exact Mass: 234.95
Molecular Weight: 236.020
Elemental Analysis: C, 30.53; H, 2.56; Br, 33.85; N, 5.93; O, 27.11
Synonym: SBA Crosslinker, SBA Cross linker, SBA Cross-linker
IUPAC/Chemical Name: N-Succinimidyl bromoacetate
InChi Key: NKUZQMZWTZAPSN-UHFFFAOYSA-N
InChi Code: InChI=1S/C6H6BrNO4/c7-3-6(11)12-8-4(9)1-2-5(8)10/h1-3H2
SMILES Code: O=C(ON1C(CCC1=O)=O)CBr
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO
Shelf Life: >2 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info:
| Biological target: | |
| In vitro activity: | |
| In vivo activity: |
The following data is based on the product molecular weight 236.02 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
| Formulation protocol: | |
| In vitro protocol: | |
| In vivo protocol: |
1: Zhao HM, Ma L, Lin D, Han KK, Chun Y, Xu QH. Preparation of cross-linked bimodal mesoporous SBA-15 with numerous pore openings. J Nanosci Nanotechnol. 2012 Dec;12(12):8955-62. PubMed PMID: 23447944.
2: Bhange P, Sridevi N, Bhange DS, Prabhune A, Ramaswamy V. Immobilization of bile salt hydrolase enzyme on mesoporous SBA-15 for co-precipitation of cholesterol. Int J Biol Macromol. 2014 Feb;63:218-24. doi: 10.1016/j.ijbiomac.2013.11.008. Epub 2013 Nov 25. PubMed PMID: 24286937.
3: Vinoba M, Bhagiyalakshmi M, Jeong SK, Yoon YI, Nam SC. Immobilization of carbonic anhydrase on spherical SBA-15 for hydration and sequestration of CO2. Colloids Surf B Biointerfaces. 2012 Feb 1;90:91-6. doi: 10.1016/j.colsurfb.2011.10.001. Epub 2011 Oct 6. PubMed PMID: 22024402.
4: Endo M, Tipper JL, Barton DC, Stone MH, Ingham E, Fisher J. Comparison of wear, wear debris and functional biological activity of moderately crosslinked and non-crosslinked polyethylenes in hip prostheses. Proc Inst Mech Eng H. 2002;216(2):111-22. PubMed PMID: 12022418.
5: Gao S, Wang Y, Diao X, Luo G, Dai Y. Effect of pore diameter and cross-linking method on the immobilization efficiency of Candida rugosa lipase in SBA-15. Bioresour Technol. 2010 Jun;101(11):3830-7. doi: 10.1016/j.biortech.2010.01.023. Epub 2010 Feb 8. PubMed PMID: 20116998.
6: Kim BC, Lee J, Um W, Kim J, Joo J, Lee JH, Kwak JH, Kim JH, Lee C, Lee H, Addleman RS, Hyeon T, Gu MB, Kim J. Magnetic mesoporous materials for removal of environmental wastes. J Hazard Mater. 2011 Sep 15;192(3):1140-7. doi: 10.1016/j.jhazmat.2011.06.022. Epub 2011 Jun 17. PubMed PMID: 21752538.
7: Zholobenko VL, Khodakov AY, Impéror-Clerc M, Durand D, Grillo I. Initial stages of SBA-15 synthesis: an overview. Adv Colloid Interface Sci. 2008 Oct 1;142(1-2):67-74. doi: 10.1016/j.cis.2008.05.003. Epub 2008 Jun 4. PubMed PMID: 18599009.
8: Baxter RM, MacDonald DW, Kurtz SM, Steinbeck MJ. Characteristics of highly cross-linked polyethylene wear debris in vivo. J Biomed Mater Res B Appl Biomater. 2013 Apr;101(3):467-75. doi: 10.1002/jbm.b.32902. Epub 2013 Feb 22. PubMed PMID: 23436587; PubMed Central PMCID: PMC3928672.
9: Godula K, Bertozzi CR. Density variant glycan microarray for evaluating cross-linking of mucin-like glycoconjugates by lectins. J Am Chem Soc. 2012 Sep 26;134(38):15732-42. Epub 2012 Sep 12. PubMed PMID: 22967056; PubMed Central PMCID: PMC3458438.
10: Fernández-Fernández M, Sanromán MÁ, Moldes D. Recent developments and applications of immobilized laccase. Biotechnol Adv. 2013 Dec;31(8):1808-25. doi: 10.1016/j.biotechadv.2012.02.013. Epub 2012 Feb 28. Review. PubMed PMID: 22398306.
11: Gupta D, Bhattacharyya L, Fant J, Macaluso F, Sabesan S, Brewer CF. Observation of unique cross-linked lattices between multiantennary carbohydrates and soybean lectin. Presence of pseudo-2-fold axes of symmetry in complex type carbohydrates. Biochemistry. 1994 Jun 21;33(24):7495-504. PubMed PMID: 8031418.
12: Dam TK, Gerken TA, Brewer CF. Thermodynamics of multivalent carbohydrate-lectin cross-linking interactions: importance of entropy in the bind and jump mechanism. Biochemistry. 2009 May 12;48(18):3822-7. doi: 10.1021/bi9002919. PubMed PMID: 19292456; PubMed Central PMCID: PMC2691598.
13: Qiang Z, Guo Y, Liu H, Cheng SZ, Cakmak M, Cavicchi KA, Vogt BD. Large-scale roll-to-roll fabrication of ordered mesoporous materials using resol-assisted cooperative assembly. ACS Appl Mater Interfaces. 2015 Feb 25;7(7):4306-10. doi: 10.1021/am5086275. Epub 2015 Feb 10. PubMed PMID: 25635350.
14: Lee J, Kim J, Kim J, Jia H, Kim MI, Kwak JH, Jin S, Dohnalkova A, Park HG, Chang HN, Wang P, Grate JW, Hyeon T. Simple synthesis of hierarchically ordered mesocellular mesoporous silica materials hosting crosslinked enzyme aggregates. Small. 2005 Jul;1(7):744-53. PubMed PMID: 17193518.
15: Dessen A, Gupta D, Sabesan S, Brewer CF, Sacchettini JC. X-ray crystal structure of the soybean agglutinin cross-linked with a biantennary analog of the blood group I carbohydrate antigen. Biochemistry. 1995 Apr 18;34(15):4933-42. PubMed PMID: 7711015.
16: Khan MI, Mandal DK, Brewer CF. Interactions of concanavalin A with glycoproteins. A quantitative precipitation study of concanavalin A with the soybean agglutinin. Carbohydr Res. 1991 Jun 25;213:69-77. PubMed PMID: 1933954.
17: Gupta D, Bhattacharyya L, Fant J, Macaluso F, Sabesan S, Brewer CF. Observation of unique cross-linked lattices between multiantennary carbohydrates and soybean lectin. Presence of pseudo-2-fold axes of symmetry in complex type carbohydrates. Biochemistry. 1994 May 10;33(18):5614-22. Corrected and republished in: Biochemistry. 1994 Jun 21;33(24):7495-504. PubMed PMID: 8180186.
18: Mencucci R, Marini M, Gheri G, Vichi D, Sarchielli E, Bonaccini L, Ambrosini S, Zappoli Thyrion GD, Paladini I, Vannelli GB, Sgambati E. Lectin binding in normal, keratoconus and cross-linked human corneas. Acta Histochem. 2011 May;113(3):308-16. doi: 10.1016/j.acthis.2009.12.003. Epub 2010 Jan 6. PubMed PMID: 20053427.
19: Olsen LR, Dessen A, Gupta D, Sabesan S, Sacchettini JC, Brewer CF. X-ray crystallographic studies of unique cross-linked lattices between four isomeric biantennary oligosaccharides and soybean agglutinin. Biochemistry. 1997 Dec 9;36(49):15073-80. PubMed PMID: 9398234.
20: Brewer CF. Lectin cross-linking interactions with multivalent carbohydrates. Adv Exp Med Biol. 2001;491:17-25. PubMed PMID: 14533787.
