WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H571630
CAS#: 910292-86-3 (sodium)
Description: BS2G-d4, or Bis(Sulfosuccinimidyl) 2,2,4,4-glutarate-d4, is a water soluble and cell membrane impermeable, protein crosslinker containing 4 deuterium atoms to provide a 4 dalton shift by mass spectrometry analysis.
Hodoodo Cat#: H571630
Name: BS2G-d4 Deuterated Crosslinker
CAS#: 910292-86-3 (sodium)
Chemical Formula: C13H8D4N2Na2O14S2
Exact Mass: 533.98
Molecular Weight: 534.363
Elemental Analysis: C, 29.22; H, 3.02; N, 5.24; Na, 8.60; O, 41.92; S, 12.00
Related CAS #: 341520-63-6 (free acid)
Synonym: D4-BS2G, BS2G-d4
IUPAC/Chemical Name: Glutaric acid-2,2,4,4-d4 bis (3-sulfo-N-hydroxysuccinimide ester) disodium salt
InChi Key: JINCQYPCQMBZSX-SNSYAXNKSA-L
InChi Code: InChI=1S/C13H14N2O14S2.2Na/c16-8-4-6(30(22,23)24)12(20)14(8)28-10(18)2-1-3-11(19)29-15-9(17)5-7(13(15)21)31(25,26)27;;/h6-7H,1-5H2,(H,22,23,24)(H,25,26,27);;/q;2*+1/p-2/i2D2,3D2;;
SMILES Code: O=C(ON1C(CC(S(=O)([O-])=O)C1=O)=O)C([2H])([2H])CC([2H])([2H])C(ON2C(CC(S(=O)([O-])=O)C2=O)=O)=O.[Na+].[Na+]
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO
Shelf Life: >2 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info: Use heavy BS2G-d4 deuterated crosslinking reagent in combination with the non-deuterated analog BS2G-d0 (BS2G-d0, BS2G) for mass spec studies. Together BS2G-d0 and BS2G-d4 can be used for protein-protein interaction mapping using Liquid Chromatography–Mass Spectrometry (LCMS, LC/MS, LC-MS).
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The following data is based on the product molecular weight 534.36 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
---|---|---|---|
1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
Formulation protocol: | |
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In vivo protocol: |
1: Haladová K, Mrázek H, Ječmen T, Halada P, Man P, Novák P, Chmelík J, Obšil T, Šulc M. The combination of hydrogen/deuterium exchange or chemical cross-linking techniques with mass spectrometry: mapping of human 14-3-3ζ homodimer interface. J Struct Biol. 2012 Jul;179(1):10-7. doi: 10.1016/j.jsb.2012.04.016. Epub 2012 May 2. PubMed PMID: 22580067.