WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H571643
CAS#: 152305-87-8 (free base)
Description: Sulfo-NHS-Acetate acylating agent, or Sulfosuccinimidyl Acetate, is a water soluble reagent that prevents polymerization when performing protein crosslinking reactions. Other Sulfo-NHS-Acetate uses include polymerization prevention when conjugating hapten peptides to carrier proteins for immunogen production.
Hodoodo Cat#: H571643
Name: Sulfo-NHS-Acetate
CAS#: 152305-87-8 (free base)
Chemical Formula: C6H7NO7S
Exact Mass: 236.99
Molecular Weight: 237.180
Elemental Analysis: C, 30.38; H, 2.97; N, 5.91; O, 47.22; S, 13.52
Related CAS #: 152305-87-8 (free base) 221222-61-3 (sodium)
Synonym: Sulfo-nhs-acetate; Sulfosuccinimidyl acetate; 1-Acetoxy-2,5-dioxopyrrolidine-3-sulfonic acid; 1-acetyloxy-2,5-dioxopyrrolidine-3-sulfonic acid
IUPAC/Chemical Name: Sulfosuccinimidyl Acetate
InChi Key: MDUQWFYJHRLNRN-UHFFFAOYSA-N
InChi Code: InChI=1S/C6H7NO7S/c1-3(8)14-7-5(9)2-4(6(7)10)15(11,12)13/h4H,2H2,1H3,(H,11,12,13)
SMILES Code: CC(ON1C(C(S(=O)(O)=O)CC1=O)=O)=O
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO
Shelf Life: >2 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info: Sulfo-NHS-Acetate irreversibly reacts with and blocks primary amines, such as in lysine, at pH greater than 7 to yield the acylated form.
Biological target: | |
In vitro activity: | |
In vivo activity: |
The following data is based on the product molecular weight 237.18 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
---|---|---|---|
1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
Formulation protocol: | |
In vitro protocol: | |
In vivo protocol: |
1: Prentice BM, Stutzman JR, McLuckey SA. Reagent cluster anions for multiple gas-phase covalent modifications of peptide and protein cations. J Am Soc Mass Spectrom. 2013 Jul;24(7):1045-52. doi: 10.1007/s13361-013-0637-6. Epub 2013 May 24. PubMed PMID: 23702708; PubMed Central PMCID: PMC3715118.
2: Lai TS, Davies C, Greenberg CS. Human tissue transglutaminase is inhibited by pharmacologic and chemical acetylation. Protein Sci. 2010 Feb;19(2):229-35. doi: 10.1002/pro.301. PubMed PMID: 19998405; PubMed Central PMCID: PMC2865712.
3: Becker D, Valk E, Zahn S, Brand P, Knop J. Coupling of contact sensitizers to thiol groups is a key event for the activation of monocytes and monocyte-derived dendritic cells. J Invest Dermatol. 2003 Feb;120(2):233-8. PubMed PMID: 12542528.
4: Guo X, Bandyopadhyay P, Schilling B, Young MM, Fujii N, Aynechi T, Guy RK, Kuntz ID, Gibson BW. Partial acetylation of lysine residues improves intraprotein cross-linking. Anal Chem. 2008 Feb 15;80(4):951-60. doi: 10.1021/ac701636w. Epub 2008 Jan 18. PubMed PMID: 18201069.