Acridine Orange
featured

    WARNING: This product is for research use only, not for human or veterinary use.

Hodoodo CAT#: H591010

CAS#: 65-61-2

Description: Acridine Orange is a cationic cytochemical stain specific for cell nuclei, especially DNA. It is used as a supravital stain and in fluorescence cytochemistry. It may cause mutations in microorganisms.


Chemical Structure

img
Acridine Orange
CAS# 65-61-2

Theoretical Analysis

Hodoodo Cat#: H591010
Name: Acridine Orange
CAS#: 65-61-2
Chemical Formula: C17H20ClN3
Exact Mass: 301.13
Molecular Weight: 301.820
Elemental Analysis: C, 67.65; H, 6.68; Cl, 11.75; N, 13.92

Price and Availability

Size Price Availability Quantity
10g USD 2450
Bulk inquiry

Synonym: Acridine Orange NS; Acridine Orange R; Basic Orange 14

IUPAC/Chemical Name: Acridine, 3,6-bis(dimethylamino)-, monohydrochloride

InChi Key: VSTHNGLPHBTRMB-UHFFFAOYSA-N

InChi Code: InChI=1S/C17H19N3.ClH/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14;/h5-11H,1-4H3;1H

SMILES Code: CN(C1=CC=C2C(N=C3C=C(N(C)C)C=CC3=C2)=C1)C.[H]Cl

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 301.82 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

1: Yu F, Zhang Y, Ye L, Tang Y, Ding G, Zhang X, Yang Z. A novel anti proliferative pentapeptide (ILYMP) isolated from Cyclina sinensis protein hydrolysate induces apoptosis of DU 145 prostate cancer cells. Mol Med Rep. 2018 May 14. doi: 10.3892/mmr.2018.9019. [Epub ahead of print] PubMed PMID: 29767237.

2: Kamalidehghan B, Ghafouri-Fard S, Motevaseli E, Ahmadipour F. Inhibition of human prostate cancer (PC-3) cells and targeting of PC-3-derived prostate cancer stem cells with koenimbin, a natural dietary compound from Murraya koenigii (L) Spreng. Drug Des Devel Ther. 2018 May 4;12:1119-1133. doi: 10.2147/DDDT.S156826. eCollection 2018. PubMed PMID: 29765202; PubMed Central PMCID: PMC5942175.

3: Vakili Zahir N, Nakhjavani M, Hajian P, Shirazi FH, Mirzaei H. Evaluation of Silibinin Effects on the Viability of HepG2 (Human hepatocellular liver carcinoma) and HUVEC (Human Umbilical Vein Endothelial) Cell Lines. Iran J Pharm Res. 2018 Winter;17(1):261-267. PubMed PMID: 29755557; PubMed Central PMCID: PMC5937096.

4: Mohammadi P, Hassani Bafrani H, Tavalaee M, Dattilo M, Nasr-Esfahani MH. One-carbon cycle support rescues sperm damages in experimentally induced varicocele in rats. BJU Int. 2018 May 11. doi: 10.1111/bju.14385. [Epub ahead of print] PubMed PMID: 29752770.

5: Patnaik R, Padhy RN. Comparative study on toxicity of methylmercury chloride and methylmercury hydroxide to the human neuroblastoma cell line SH-SY5Y. Environ Sci Pollut Res Int. 2018 May 11. doi: 10.1007/s11356-018-2164-2. [Epub ahead of print] PubMed PMID: 29752667.

6: Zheng Y, Zheng Z, Ming Y, Lin M, Chen L, Huang W, Xiao J, Lin H. Gynosaponin TN-1 producing from the enzymatic conversion of gypenoside XLVI by naringinase and its cytotoxicity on hepatoma cell lines. Food Chem Toxicol. 2018 May 8. pii: S0278-6915(18)30297-7. doi: 10.1016/j.fct.2018.05.007. [Epub ahead of print] PubMed PMID: 29751078.

7: Liu S, Li X, Li Q, Liu H, Shi Y, Zhuo H, Li C, Zhu H. Silencing Livin improved the sensitivity of colon cancer cells to 5-fluorouracil by regulating crosstalk between apoptosis and autophagy. Oncol Lett. 2018 May;15(5):7707-7715. doi: 10.3892/ol.2018.8282. Epub 2018 Mar 16. PubMed PMID: 29740490; PubMed Central PMCID: PMC5934728.

8: Sudha A, Srinivasan P, Kanimozhi V, Palanivel K, Kadalmani B. Antiproliferative and apoptosis-induction studies of 5-hydroxy 3',4',7-trimethoxyflavone in human breast cancer cells MCF-7: an in vitro and in silico approach. J Recept Signal Transduct Res. 2018 May 8:1-12. doi: 10.1080/10799893.2018.1468780. [Epub ahead of print] PubMed PMID: 29734849.

9: Almeida D, Pinho R, Correia V, Soares J, Bastos ML, Carvalho F, Capela JP, Costa VM. Mitoxantrone is More Toxic than Doxorubicin in SH-SY5Y Human Cells: A 'Chemobrain' In Vitro Study. Pharmaceuticals (Basel). 2018 May 5;11(2). pii: E41. doi: 10.3390/ph11020041. PubMed PMID: 29734752.

10: Yang HB, Gao HR, Ren YJ, Fang FX, Tian HT, Gao ZJ, Song W, Huang SM, Zhao AF. Effects of isoimperatorin on proliferation and apoptosis of human gastric carcinoma cells. Oncol Lett. 2018 May;15(5):7993-7998. doi: 10.3892/ol.2018.8303. Epub 2018 Mar 20. PubMed PMID: 29731910; PubMed Central PMCID: PMC5920862.

11: Jaafaru MS, Nordin N, Shaari K, Rosli R, Abdull Razis AF. Isothiocyanate from Moringa oleifera seeds mitigates hydrogen peroxide-induced cytotoxicity and preserved morphological features of human neuronal cells. PLoS One. 2018 May 3;13(5):e0196403. doi: 10.1371/journal.pone.0196403. eCollection 2018. PubMed PMID: 29723199.

12: Želježić D, Žunec S, Bjeliš M, Benković V, Mladinić M, Lovaković Tariba B, Pavičić I, Marjanović Čermak AM, Kašuba V, Milić M, Pizent A, Lucić Vrdoljak A, Kopjar N. Effects of the chloro-s-triazine herbicide terbuthylazine on DNA integrity in human and mouse cells. Environ Sci Pollut Res Int. 2018 May 2. doi: 10.1007/s11356-018-2046-7. [Epub ahead of print] PubMed PMID: 29721798.

13: Cheng SY, Chen NF, Kuo HM, Yang SN, Sung CS, Sung PJ, Wen ZH, Chen WF. Prodigiosin stimulates endoplasmic reticulum stress and induces autophagic cell death in glioblastoma cells. Apoptosis. 2018 May 2. doi: 10.1007/s10495-018-1456-9. [Epub ahead of print] PubMed PMID: 29721785.

14: Zhou Y, Quan G, Wu Q, Zhang X, Niu B, Wu B, Huang Y, Pan X, Wu C. Mesoporous silica nanoparticles for drug and gene delivery. Acta Pharm Sin B. 2018 Mar;8(2):165-177. doi: 10.1016/j.apsb.2018.01.007. Epub 2018 Feb 12. Review. PubMed PMID: 29719777; PubMed Central PMCID: PMC5926503.

15: Hsiao YT, Kuo CL, Lin JJ, Huang WW, Peng SF, Chueh FS, Bau DT, Chung JG. Curcuminoids combined with gefitinib mediated apoptosis and autophagy of human oral cancer SAS cells in vitro and reduced tumor of SAS cell xenograft mice in vivo. Environ Toxicol. 2018 May 2. doi: 10.1002/tox.22568. [Epub ahead of print] PubMed PMID: 29717538.

16: Panner Selvam MK, Agarwal A. A systematic review on sperm DNA fragmentation in male factor infertility: Laboratory assessment. Arab J Urol. 2018 Jan 17;16(1):65-76. doi: 10.1016/j.aju.2017.12.001. eCollection 2018 Mar. PubMed PMID: 29713537; PubMed Central PMCID: PMC5922187.

17: Zhao J, Zhong F, Yu H, Chen Z, Wang M, Chen J. Human cytomegalovirus infection-induced autophagy was associated with the biological behavioral changes of human umbilical vein endothelial cell (HUVEC). Biomed Pharmacother. 2018 Jun;102:938-946. doi: 10.1016/j.biopha.2018.03.156. Epub 2018 Apr 5. PubMed PMID: 29710549.

18: Kocyigit A, Guler EM, Karatas E, Caglar H, Bulut H. Dose-dependent proliferative and cytotoxic effects of melatonin on human epidermoid carcinoma and normal skin fibroblast cells. Mutat Res. 2018 May - Jun;829-830:50-60. doi: 10.1016/j.mrgentox.2018.04.002. Epub 2018 Apr 10. PubMed PMID: 29704993.

19: Sonia, Komal, Kukreti S, Kaushik M. Exploring the DNA damaging potential of chitosan and citrate-reduced gold nanoparticles: Physicochemical approach. Int J Biol Macromol. 2018 Apr 24;115:801-810. doi: 10.1016/j.ijbiomac.2018.04.115. [Epub ahead of print] PubMed PMID: 29698763.

20: Akyuva Y, Kaplan N, Yilmaz I, Ozbek H, Sirin DY, Karaaslan N, Guler O, Ateş Ö. Delivering growth factors through a polymeric scaffold to cell cultures containing both nucleus pulposus and annulus fibrosus. Turk Neurosurg. 2018 Apr 9. doi: 10.5137/1019-5149.JTN.22672-18.1. [Epub ahead of print] PubMed PMID: 29694659.