MBED

    WARNING: This product is for research use only, not for human or veterinary use.

Hodoodo CAT#: H562817

CAS#: 123363-40-6

Description: MBED is a potent RyR agonist.


Chemical Structure

img
MBED
CAS# 123363-40-6

Theoretical Analysis

Hodoodo Cat#: H562817
Name: MBED
CAS#: 123363-40-6
Chemical Formula: C12H8Br2N2O
Exact Mass: 353.90
Molecular Weight: 356.010
Elemental Analysis: C, 40.48; H, 2.27; Br, 44.89; N, 7.87; O, 4.49

Price and Availability

This product is not in stock, which may be available by custom synthesis. For cost-effective reason, minimum order is 1g (price is usually high, lead time is 2~3 months, depending on the technical challenge). Quote less than 1g will not be provided. To request quote, please email to sales @hodoodo.com or click below button.
Note: Price will be listed if it is available in the future.

Request quote for custom synthesis

Synonym: MBED;

IUPAC/Chemical Name: 5,7-Dibromo-9-methylpyrido[3,4-b]indol-6-ol

InChi Key: BFWFPORISPXHAR-UHFFFAOYSA-N

InChi Code: InChI=1S/C12H8Br2N2O/c1-16-8-4-7(13)12(17)11(14)10(8)6-2-3-15-5-9(6)16/h2-5,17H,1H3

SMILES Code: OC1=C(Br)C2=C(N(C)C3=C2C=CN=C3)C=C1Br

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 356.01 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

1: Inoue S, Li WY, Tseng A, Beerman I, Elia AJ, Bendall SC, Lemonnier F, Kron KJ, Cescon DW, Hao Z, Lind EF, Takayama N, Planello AC, Shen SY, Shih AH, Larsen DM, Li Q, Snow BE, Wakeham A, Haight J, Gorrini C, Bassi C, Thu KL, Murakami K, Elford AR, Ueda T, Straley K, Yen KE, Melino G, Cimmino L, Aifantis I, Levine RL, De Carvalho DD, Lupien M, Rossi DJ, Nolan GP, Cairns RA, Mak TW. Mutant IDH1 Downregulates ATM and Alters DNA Repair and Sensitivity to DNA Damage Independent of TET2. Cancer Cell. 2016 Aug 8;30(2):337-348. doi: 10.1016/j.ccell.2016.05.018. Epub 2016 Jul 14. PubMed PMID: 27424808; PubMed Central PMCID: PMC5022794.

2: Hao Z, Cairns RA, Inoue S, Li WY, Sheng Y, Lemonnier F, Wakeham A, Snow BE, Dominguez-Brauer C, Ye J, Larsen DM, Straley KS, Tobin ER, Narayanaswamy R, Gaulard P, Mak TW. Idh1 mutations contribute to the development of T-cell malignancies in genetically engineered mice. Proc Natl Acad Sci U S A. 2016 Feb 2;113(5):1387-92. doi: 10.1073/pnas.1525354113. Epub 2016 Jan 19. PubMed PMID: 26787889; PubMed Central PMCID: PMC4747700.

3: Jehl P, Sievers F, Higgins DG. OD-seq: outlier detection in multiple sequence alignments. BMC Bioinformatics. 2015 Aug 25;16:269. doi: 10.1186/s12859-015-0702-1. PubMed PMID: 26303676; PubMed Central PMCID: PMC4548304.

4: Diaz-Sylvester PL, Porta M, Juettner VV, Lv Y, Fleischer S, Copello JA. Eudistomin D and penaresin derivatives as modulators of ryanodine receptor channels and sarcoplasmic reticulum Ca2+ ATPase in striated muscle. Mol Pharmacol. 2014 Apr;85(4):564-75. doi: 10.1124/mol.113.089342. Epub 2014 Jan 14. PubMed PMID: 24423447; PubMed Central PMCID: PMC3965891.

5: Lebrero R, Gondim AC, Pérez R, García-Encina PA, Muñoz R. Comparative assessment of a biofilter, a biotrickling filter and a hollow fiber membrane bioreactor for odor treatment in wastewater treatment plants. Water Res. 2014 Feb 1;49:339-50. doi: 10.1016/j.watres.2013.09.055. Epub 2013 Oct 10. PubMed PMID: 24295931.

6: Sievers F, Higgins DG. Clustal Omega, accurate alignment of very large numbers of sequences. Methods Mol Biol. 2014;1079:105-16. doi: 10.1007/978-1-62703-646-7_6. PubMed PMID: 24170397.

7: Macías Macías M, Agudo JE, García Manso A, García Orellana CJ, González Velasco HM, Gallardo Caballero R. A compact and low cost electronic nose for aroma detection. Sensors (Basel). 2013 Apr 25;13(5):5528-41. doi: 10.3390/s130505528. PubMed PMID: 23698265; PubMed Central PMCID: PMC3690013.

8: Pedicini P, Caivano R, Jereczek-Fossa BA, Strigari L, Vischioni B, Alterio D, Cremonesi M, Botta F, Nappi A, Improta G, Storto G, Benassi M, Orecchia R. Modelling the correlation between EGFr expression and tumour cell radiosensitivity, and combined treatments of radiation and monoclonal antibody EGFr inhibitors. Theor Biol Med Model. 2012 Jun 19;9:23. doi: 10.1186/1742-4682-9-23. PubMed PMID: 22713695; PubMed Central PMCID: PMC3502488.

9: Blackshields G, Sievers F, Shi W, Wilm A, Higgins DG. Sequence embedding for fast construction of guide trees for multiple sequence alignment. Algorithms Mol Biol. 2010 May 14;5:21. doi: 10.1186/1748-7188-5-21. PubMed PMID: 20470396; PubMed Central PMCID: PMC2893182.

10: Lee IH, Eisbruch A. Mucositis versus tumor control: the therapeutic index of adding chemotherapy to irradiation of head and neck cancer. Int J Radiat Oncol Biol Phys. 2009 Nov 15;75(4):1060-3. doi: 10.1016/j.ijrobp.2008.12.011. Epub 2009 Mar 21. PubMed PMID: 19304406.

11: Gustafsson AJ, Ingelman-Sundberg H, Dzabic M, Awasum J, Nguyen KH, Ostenson CG, Pierro C, Tedeschi P, Woolcott O, Chiounan S, Lund PE, Larsson O, Islam MS. Ryanodine receptor-operated activation of TRP-like channels can trigger critical Ca2+ signaling events in pancreatic beta-cells. FASEB J. 2005 Feb;19(2):301-3. Epub 2004 Nov 30. PubMed PMID: 15572434.

12: Bruton JD, Lemmens R, Shi CL, Persson-Sjögren S, Westerblad H, Ahmed M, Pyne NJ, Frame M, Furman BL, Islam MS. Ryanodine receptors of pancreatic beta-cells mediate a distinct context-dependent signal for insulin secretion. FASEB J. 2003 Feb;17(2):301-3. Epub 2002 Dec 3. PubMed PMID: 12475892.

13: Brüske J, Bednarski M, Grzelec H, Zyluk A. The usefulness of the Phalen test and the Hoffmann-Tinel sign in the diagnosis of carpal tunnel syndrome. Acta Orthop Belg. 2002 Apr;68(2):141-5. PubMed PMID: 12050999.

14: Ohi Y, Atsuki K, Tori Y, Ohizumi Y, Watanabe M, Imaizumi Y. Imaging of Ca2+ release by caffeine and 9-methyl-7-bromoeudistomin D and the associated activation of large conductance Ca2+-dependent K+ channels in urinary bladder smooth muscle cells of the guinea pig. Jpn J Pharmacol. 2001 Apr;85(4):382-90. PubMed PMID: 11388642.

15: Ohizumi Y, Hirata Y, Suzuki A, Kobayashi M. Two novel types of calcium release from skeletal sarcoplasmic reticulum by phosphatidylinositol 4,5-biphosphate. Can J Physiol Pharmacol. 1999 Apr;77(4):276-85. PubMed PMID: 10535676.

16: Seino-Umeda A, Fang YI, Ishibashi M, Kobayashi J, Ohizumi Y. 9-Methyl-7-bromoeudistomin D induces Ca2+ release from cardiac sarcoplasmic reticulum. Eur J Pharmacol. 1998 Sep 18;357(2-3):261-5. PubMed PMID: 9797046.

17: Tadano T, Takahashi N, Nakagawasai O, Tan-no K, Kaneko S, Matsunaga K, Ohizumi Y, Kisara K. Central action of 9-methyl-7-bromoeudistomin D (MBED), a derivative of eudistomin D isolated from Eudistoma olivaceum. Methods Find Exp Clin Pharmacol. 1998 Jan-Feb;20(1):53-8. PubMed PMID: 9575483.

18: Lahouratate P, Guibert J, Faivre JF. cADP-ribose releases Ca2+ from cardiac sarcoplasmic reticulum independently of ryanodine receptor. Am J Physiol. 1997 Sep;273(3 Pt 2):H1082-9. PubMed PMID: 9321792.

19: Imaizumi Y, Henmi S, Uyama Y, Atsuki K, Torii Y, Ohizumi Y, Watanabe M. Characteristics of Ca2+ release for activation of K+ current and contractile system in some smooth muscles. Am J Physiol. 1996 Sep;271(3 Pt 1):C772-82. PubMed PMID: 8843706.

20: Yamada Y, Yamada M, Nakazawa A. A ColE1-encoded gene directs entry exclusion of the plasmid. J Bacteriol. 1995 Nov;177(21):6064-8. PubMed PMID: 7592368; PubMed Central PMCID: PMC177443.