WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H462989
CAS#: 84256-90-6
Description: Aminobenzyl-EDTA has been used to develop an ELISA test for the detection of mercury in water. Aminobenzyl-EDTA has also been used in a study to provide a new class of affinity cleaving reagents that can be directed against protein and nucleic acid targets.
Hodoodo Cat#: H462989
Name: Aminobenzyl-edta
CAS#: 84256-90-6
Chemical Formula: C17H23N3O8
Exact Mass: 397.15
Molecular Weight: 397.384
Elemental Analysis: C, 51.38; H, 5.83; N, 10.57; O, 32.21
Synonym: Aminobenzyl-edta;
IUPAC/Chemical Name: 2,2',2'',2'''-((3-(4-aminophenyl)propane-1,2-diyl)bis(azanetriyl))tetraacetic acid
InChi Key: RPBJIOJIMOUOIN-UHFFFAOYSA-N
InChi Code: InChI=1S/C17H23N3O8/c18-12-3-1-11(2-4-12)5-13(20(9-16(25)26)10-17(27)28)6-19(7-14(21)22)8-15(23)24/h1-4,13H,5-10,18H2,(H,21,22)(H,23,24)(H,25,26)(H,27,28)
SMILES Code: Nc1ccc(CC(N(CC(O)=O)CC(O)=O)CN(CC(O)=O)CC(O)=O)cc1
Appearance: Solid powder
Purity: >90% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO
Shelf Life: >3 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info:
| Biological target: | |
| In vitro activity: | |
| In vivo activity: |
The following data is based on the product molecular weight 397.38 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
| Formulation protocol: | |
| In vitro protocol: | |
| In vivo protocol: |
1: Kong T, Hao XQ, Li XB, Liu GW, Zhang ZG, Yang ZJ, Wang Z, Tang J, Yang W, Sun J. Preparation of novel monoclonal antibodies against chelated cadmium ions. Biol Trace Elem Res. 2013 Apr;152(1):117-24. doi: 10.1007/s12011-013-9599-6. Epub 2013 Feb 2. PMID: 23377609.
2: Zhang Y, Li X, Liu G, Wang Z, Kong T, Tang J, Zhag P, Yang W, Li D, Liu L, Xie G, Wang J. Development of ELISA for detection of mercury based on specific monoclonal antibodies against mercury-chelate. Biol Trace Elem Res. 2011 Dec;144(1-3):854-64. doi: 10.1007/s12011-011-9031-z. Epub 2011 Mar 25. PMID: 21437665.
3: Zhao H, Xue C, Nan T, Tan G, Li Z, Li QX, Zhang Q, Wang B. Detection of copper ions using microcantilever immunosensors and enzyme-linked immunosorbent assay. Anal Chim Acta. 2010 Aug 31;676(1-2):81-6. doi: 10.1016/j.aca.2010.07.041. Epub 2010 Aug 3. PMID: 20800746.
4: Delehanty JB, Jones RM, Bishop TC, Blake DA. Identification of important residues in metal-chelate recognition by monoclonal antibodies. Biochemistry. 2003 Dec 9;42(48):14173-83. doi: 10.1021/bi034839d. PMID: 14640685.
5: Chmura AJ, Orton MS, Meares CF. Antibodies with infinite affinity. Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8480-4. doi: 10.1073/pnas.151260298. Epub 2001 Jul 10. PMID: 11447282; PMCID: PMC37461.
6: Huq I, Rana TM. Probing the proximity of the core domain of an HIV-1 Tat fragment in a Tat-TAR complex by affinity cleaving. Biochemistry. 1997 Oct 14;36(41):12592-9. doi: 10.1021/bi971011g. PMID: 9376365.
7: Song AI, Rana TM. Synthesis of an amino acid analogue to incorporate p-aminobenzyl-EDTA in peptides. Bioconjug Chem. 1997 Mar-Apr;8(2):249-52. doi: 10.1021/bc960084u. PMID: 9095368.
8: Studer M, Kroger LA, DeNardo SJ, Kukis DL, Meares CF. Influence of a peptide linker on biodistribution and metabolism of antibody-conjugated benzyl-EDTA. Comparison of enzymatic digestion in vitro and in vivo. Bioconjug Chem. 1992 Sep-Oct;3(5):424-9. doi: 10.1021/bc00017a012. PMID: 1420442.
9: Studer M, Meares CF. A convenient and flexible approach for introducing linkers on bifunctional chelating agents. Bioconjug Chem. 1992 Sep- Oct;3(5):420-3. doi: 10.1021/bc00017a011. PMID: 1420441.
10: Thakur ML, Richard MD, White FW 3rd. Monoclonal antibodies as agents for selective radiolabeling of human neutrophils. J Nucl Med. 1988 Nov;29(11):1817-25. PMID: 3141596.
