WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H522394
CAS#: 832115-62-5
Description: ISX-9 is a neurogenic agent. Isx-9 promotes neurogenesis in vivo, enhancing the proliferation and differentiation of hippocampal subgranular zone (SGZ) neuroblasts, and the dendritic arborization of adult-generated dentate gyrus neurons. Isx-9 also improves hippocampal function, enhancing memory in the Morris water maze. Notably, Isx-9 enhances neurogenesis and memory without detectable increases in cellular or animal activity or vascularization. Isx-9 enhances hippocampal neurogenesis and memory in vivo, and its effects are reliant on Mef2, revealing a novel cell-intrinsic molecular pathway regulating adult neurogenesis.
Hodoodo Cat#: H522394
Name: ISX-9
CAS#: 832115-62-5
Chemical Formula: C11H10N2O2S
Exact Mass: 234.05
Molecular Weight: 234.270
Elemental Analysis: C, 56.40; H, 4.30; N, 11.96; O, 13.66; S, 13.68
Synonym: ISX-9; ISX 9; ISX9; Isoxazole 9
IUPAC/Chemical Name: N-Cyclopropyl-5-(2-thienyl)-3-isoxazolecarboxamide
InChi Key: SYENTKHGMVKGAQ-UHFFFAOYSA-N
InChi Code: InChI=1S/C11H10N2O2S/c14-11(12-7-3-4-7)8-6-9(15-13-8)10-2-1-5-16-10/h1-2,5-7H,3-4H2,(H,12,14)
SMILES Code: O=C(C1=NOC(C2=CC=CS2)=C1)NC3CC3
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO, not in water
Shelf Life: >2 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info:
| Biological target: | ISX-9 activates Ca2+ influx through both voltage-gated Ca2+ channels and NMDA receptors and increases neuroD expression. |
| In vitro activity: | Isx-9 was examined for its effects on the three major populations of progenitor/precursor cells in brain: NSPCs, oligodendrocyte precursor cells (OPCs), and endothelial progenitor cells (EPCs). Cultured primary NSPCs, OPCs, or EPCs were treated with various concentrations of Isx-9 (6.25, 12.5, 25, 50 μM), and their cell numbers were counted in a blinded manner. Isx-9 slightly increased the number of NSPCs and effectively induced neuronal differentiation of NSPCs. However, Isx-9 significantly decreased OPC number in a concentration-dependent manner, suggesting cytotoxicity. Isx-9 did not affect EPC cell number. But in a matrigel assay of angiogenesis, Isx-9 significantly inhibited tube formation in outgrowth endothelial cells derived from EPCs. This potential anti-tube-formation effect of Isx-9 was confirmed in a brain endothelial cell line. Taken together, the data suggests that mechanisms and targets for promoting stem/progenitor cells in the central nervous system may significantly differ between cell types. Reference: PLoS One. 2015 Sep 25;10(9):e0138724. https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/26407349/ |
| In vivo activity: | Isx-9 promotes neurogenesis in vivo, enhancing the proliferation and differentiation of hippocampal subgranular zone (SGZ) neuroblasts, and the dendritic arborization of adult-generated dentate gyrus neurons. Isx-9 also improves hippocampal function, enhancing memory in the Morris water maze. Notably, Isx-9 enhances neurogenesis and memory without detectable increases in cellular or animal activity or vascularization. Molecular exploration of Isx-9-induced regulation of neurogenesis (via FACS and microarray of SGZ stem and progenitor cells) suggested the involvement of the myocyte-enhancer family of proteins (Mef2). Indeed, transgenic-mediated inducible knockout of all brain-enriched Mef2 isoforms (Mef2a/c/d) specifically from neural stem cells and their progeny confirmed Mef2's requirement for Isx-9-induced increase in hippocampal neurogenesis. Thus, Isx-9 enhances hippocampal neurogenesis and memory in vivo, and its effects are reliant on Mef2, revealing a novel cell-intrinsic molecular pathway regulating adult neurogenesis. Reference: FASEB J. 2012 Aug;26(8):3148-62. https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22542682/ |
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 46.0 | 196.35 | |
| Ethanol | 13.0 | 55.49 |
The following data is based on the product molecular weight 234.27 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
| Formulation protocol: | |
| In vitro protocol: | 1. Koh SH, Liang AC, Takahashi Y, Maki T, Shindo A, Osumi N, Zhao J, Lin H, Holder JC, Chuang TT, McNeish JD, Arai K, Lo EH. Differential Effects of Isoxazole-9 on Neural Stem/Progenitor Cells, Oligodendrocyte Precursor Cells, and Endothelial Progenitor Cells. PLoS One. 2015 Sep 25;10(9):e0138724. doi: 10.1371/journal.pone.0138724. PMID: 26407349; PMCID: PMC4583469. |
| In vivo protocol: | 1. Petrik D, Jiang Y, Birnbaum SG, Powell CM, Kim MS, Hsieh J, Eisch AJ. Functional and mechanistic exploration of an adult neurogenesis-promoting small molecule. FASEB J. 2012 Aug;26(8):3148-62. doi: 10.1096/fj.11-201426. Epub 2012 Apr 27. PMID: 22542682; PMCID: PMC3405259. |
1: Petrik D, Jiang Y, Birnbaum SG, Powell CM, Kim MS, Hsieh J, Eisch AJ.
Functional and mechanistic exploration of an adult neurogenesis-promoting small
molecule. FASEB J. 2012 Aug;26(8):3148-62. doi: 10.1096/fj.11-201426. Epub 2012
Apr 27. PubMed PMID: 22542682; PubMed Central PMCID: PMC3405259.
