WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H522470
CAS#: 349438-38-6
Description: Alda-1 is a potent and selective Aldehyde Dehydrogenase-2 Agonist. Alda-1 reverses alcohol-induced hepatic steatosis and cell death in mice. Alda-1 inhibits atherosclerosis and attenuates hepatic steatosis in apolipoprotein E-knockout mice. Alda-1 reduces cerebral ischemia/reperfusion injury in rat through clearance of reactive aldehydes. Pharmacological activation of ALDH2 by Alda-1 reversed alcoholic steatosis and apoptosis through accelerating aldehyde clearance. This study indicates that ALDH2 is a promising molecular target and Alda-1 has therapeutic potential for treating alcoholic liver disease.
Hodoodo Cat#: H522470
Name: Alda-1
CAS#: 349438-38-6
Chemical Formula: C15H11Cl2NO3
Exact Mass: 323.01
Molecular Weight: 324.160
Elemental Analysis: C, 55.58; H, 3.42; Cl, 21.87; N, 4.32; O, 14.81
Synonym: Alda-1; Alda1; Alda 1.
IUPAC/Chemical Name: N-(1,3-Benzodioxol-5-ylmethyl)-2,6-dichlorobenzamide
InChi Key: NMKJFZCBCIUYHI-UHFFFAOYSA-N
InChi Code: InChI=1S/C15H11Cl2NO3/c16-10-2-1-3-11(17)14(10)15(19)18-7-9-4-5-12-13(6-9)21-8-20-12/h1-6H,7-8H2,(H,18,19)
SMILES Code: O=C(NCC1=CC=C(OCO2)C2=C1)C3=C(Cl)C=CC=C3Cl
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO, not in water
Shelf Life: >2 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info:
Biological target: | |
In vitro activity: | |
In vivo activity: |
The following data is based on the product molecular weight 324.16 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
---|---|---|---|
1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
Formulation protocol: | |
In vitro protocol: | |
In vivo protocol: |
1: Sun X, Gao R, Li W, Zhao Y, Yang H, Chen H, Jiang H, Dong Z, Hu J, Liu J, Zou Y, Sun A, Ge J. Alda-1 treatment promotes the therapeutic effect of mitochondrial transplantation for myocardial ischemia-reperfusion injury. Bioact Mater. 2021 Jan 8;6(7):2058-2069. doi: 10.1016/j.bioactmat.2020.12.024. PMID: 33511307; PMCID: PMC7809100.
2: Wu H, Xu S, Diao M, Wang J, Zhang G, Xu J. ALDA-1 TREATMENT ALLEVIATES LUNG INJURY AFTER CARDIAC ARREST AND RESUSCITATION IN SWINE. Shock. 2022 Nov 1;58(5):464-469. doi: 10.1097/SHK.0000000000002003. Epub 2022 Sep 27. PMID: 36156537.
3: Samuvel DJ, Li L, Krishnasamy Y, Gooz M, Takemoto K, Woster PM, Lemasters JJ, Zhong Z. Mitochondrial depolarization after acute ethanol treatment drives mitophagy in living mice. Autophagy. 2022 Nov;18(11):2671-2685. doi: 10.1080/15548627.2022.2046457. Epub 2022 Mar 16. PMID: 35293288; PMCID: PMC9629059.
4: Sidramagowda Patil S, Hernández-Cuervo H, Fukumoto J, Krishnamurthy S, Lin M, Alleyn M, Breitzig M, Narala VR, Soundararajan R, Lockey RF, Kolliputi N, Galam L. Alda-1 Attenuates Hyperoxia-Induced Acute Lung Injury in Mice. Front Pharmacol. 2021 Jan 8;11:597942. doi: 10.3389/fphar.2020.597942. PMID: 33597876; PMCID: PMC7883597.
5: Liu YC, Wang HL, Huang YZ, Weng YH, Chen RS, Tsai WC, Yeh TH, Lu CS, Chen YL, Lin YW, Chen YJ, Hsu CC, Chiu CH, Chiu CC. Alda-1, an activator of ALDH2, ameliorates Achilles tendinopathy in cellular and mouse models. Biochem Pharmacol. 2020 May;175:113919. doi: 10.1016/j.bcp.2020.113919. Epub 2020 Mar 17. PMID: 32194057.
6: Yu Q, Gao J, Shao X, Lu W, Chen L, Jin L. The Effects of Alda-1 Treatment on Renal and Intestinal Injuries After Cardiopulmonary Resuscitation in Pigs. Front Med (Lausanne). 2022 May 12;9:892472. doi: 10.3389/fmed.2022.892472. PMID: 35646953; PMCID: PMC9133723.
7: Diao M, Xu J, Wang J, Zhang M, Wu C, Hu X, Zhu Y, Zhang M, Hu W. Alda-1, an Activator of ALDH2, Improves Postresuscitation Cardiac and Neurological Outcomes by Inhibiting Pyroptosis in Swine. Neurochem Res. 2022 Apr;47(4):1097-1109. doi: 10.1007/s11064-021-03511-x. Epub 2022 Jan 30. PMID: 35094247.
8: Zhang S, Chen C, Ying J, Wei C, Wang L, Yang Z, Qi F. Alda-1, an Aldehyde Dehydrogenase 2 Agonist, Improves Cutaneous Wound Healing by Activating Epidermal Keratinocytes via Akt/GSK-3β/β-Catenin Pathway. Aesthetic Plast Surg. 2020 Jun;44(3):993-1005. doi: 10.1007/s00266-020-01614-4. Epub 2020 Jan 17. PMID: 31953581.
9: Belmont-Díaz JA, Yoval-Sánchez B, Calleja-Castañeda LF, Pardo Vázquez JP, Rodríguez-Zavala JS. Alda-1 modulates the kinetic properties of mitochondrial aldehyde dehydrogenase (ALDH2). FEBS J. 2016 Oct;283(19):3637-3650. doi: 10.1111/febs.13833. PMID: 27521998.
10: Khan M, Qiao F, Kumar P, Touhidul Islam SM, Singh AK, Won J, Singh I. Neuroprotective effects of Alda-1 mitigate spinal cord injury in mice: involvement of Alda-1-induced ALDH2 activation-mediated suppression of reactive aldehyde mechanisms. Neural Regen Res. 2022 Jan;17(1):185-193. doi: 10.4103/1673-5374.314312. PMID: 34100455; PMCID: PMC8451565.
11: Zhang SM, Yang Z, Qi FZ. Alda-1, an Aldehyde Dehydrogenase 2 Agonist, Improves Cutaneous Wound Healing by Activating Epidermal Keratinocytes via Akt/GSK-3β/β-Catenin Pathway. Aesthetic Plast Surg. 2022 Apr;46(Suppl 1):126-127. doi: 10.1007/s00266-021-02550-7. Epub 2021 Aug 30. PMID: 34462796.
12: Patil SS, Hernández-Cuervo H, Fukumoto J, Narala VR, Saji S, Borra M, Alleyn M, Lin M, Soundararajan R, Lockey R, Kolliputi N, Galam L. Alda-1 attenuates hyperoxia-induced mitochondrial dysfunction in lung vascular endothelial cells. Aging (Albany NY). 2019 Jun 17;11(12):3909-3918. doi: 10.18632/aging.102012. PMID: 31209184; PMCID: PMC6628993.
13: Dong X, Zhang M, Jin X. Alda-1, an Aldehyde Dehydrogenase 2 Agonist, Improves Cutaneous Wound Healing by Activating Epidermal Keratinocytes Via Akt/GSK-3β/β-Catenin Pathway. Aesthetic Plast Surg. 2022 Apr;46(Suppl 1):125. doi: 10.1007/s00266-021-02536-5. Epub 2021 Aug 25. PMID: 34435221.
14: Hua T, Yang M, Zhou Y, Chen L, Wu H, Liu R. Alda-1 Prevents Pulmonary Epithelial Barrier Dysfunction following Severe Hemorrhagic Shock through Clearance of Reactive Aldehydes. Biomed Res Int. 2019 Aug 4;2019:2476252. doi: 10.1155/2019/2476252. PMID: 31467875; PMCID: PMC6699483.
15: Zhang T, Zhao Q, Ye F, Huang CY, Chen WM, Huang WQ. Alda-1, an ALDH2 activator, protects against hepatic ischemia/reperfusion injury in rats via inhibition of oxidative stress. Free Radic Res. 2018 Jun;52(6):629-638. doi: 10.1080/10715762.2018.1459042. Epub 2018 Apr 13. PMID: 29589772.
16: Hammad FT, Al-Salam S, Yuvaraju P, Lubbad L. Alda-1, an aldehyde dehydrogenase-2 agonist, causes deterioration in renal functions following ischemia-reperfusion injury due to crystalline nephropathy. Drug Dev Res. 2018 Nov;79(7):315-323. doi: 10.1002/ddr.21454. Epub 2018 Oct 6. PMID: 30291750.
17: Li M, Xu M, Li J, Chen L, Xu D, Tong Y, Zhang J, Wu H, Kong X, Xia Q. Alda-1 Ameliorates Liver Ischemia-Reperfusion Injury by Activating Aldehyde Dehydrogenase 2 and Enhancing Autophagy in Mice. J Immunol Res. 2018 Dec 24;2018:9807139. doi: 10.1155/2018/9807139. PMID: 30671488; PMCID: PMC6323494.
18: Ding J, Zhang Q, Luo Q, Ying Y, Liu Y, Li Y, Wei W, Yan F, Zhang H. Alda-1 Attenuates Lung Ischemia-Reperfusion Injury by Reducing 4-Hydroxy-2-Nonenal in Alveolar Epithelial Cells. Crit Care Med. 2016 Jul;44(7):e544-52. doi: 10.1097/CCM.0000000000001563. PMID: 26757166.
19: Islam SMT, Won J, Kim J, Qiao F, Singh AK, Khan M, Singh I. Detoxification of Reactive Aldehydes by Alda-1 Treatment Ameliorates Experimental Autoimmune Encephalomyelitis in Mice. Neuroscience. 2021 Mar 15;458:31-42. doi: 10.1016/j.neuroscience.2021.01.021. Epub 2021 Jan 23. PMID: 33493617.
20: Hua Y, Chen H, Zhao X, Liu M, Jin W, Yan W, Wu Y, Tan Z, Fan H, Wu Y, Xie L, Zhang W, Liu B, Zhou Y. Alda‑1, an aldehyde dehydrogenase‑2 agonist, improves long‑term survival in rats with chronic heart failure following myocardial infarction. Mol Med Rep. 2018 Sep;18(3):3159-3166. doi: 10.3892/mmr.2018.9309. Epub 2018 Jul 24. PMID: 30066916; PMCID: PMC6102689.