Diloxanide
featured

    WARNING: This product is for research use only, not for human or veterinary use.

Hodoodo CAT#: H317616

CAS#: 579-38-4

Description: Diloxanide is a luminal amebicide used in the treatment of amebiasis. It is considered the luminal agent of choice for mild intestinal amebiasis or asymptomatic cyst carriers. It can also be added to metronidazole (active drug in luminal and extraintestinal amebiasis) in acute amebic dysentery as well as hepatic abscess (In hepatic abscess it is for the control of cysts in the lumen which may cause relapse).

Chemical Structure

img
Diloxanide
CAS# 579-38-4
Instruction

Theoretical Analysis

Hodoodo Cat#: H317616
Name: Diloxanide
CAS#: 579-38-4
Chemical Formula: C9H9Cl2NO2
Exact Mass: 233.00
Molecular Weight: 234.076
Elemental Analysis: C, 46.18; H, 3.88; Cl, 30.29; N, 5.98; O, 13.67

Price and Availability

Size Price Availability Quantity
50mg USD 250 2 weeks
Bulk inquiry

Synonym: RD 3803; RD-3803; RD3803; Diloxanide; Diloxanid; Diloxanida

IUPAC/Chemical Name: 2,2-dichloro-N-(4-hydroxyphenyl)-N-methylacetamide

InChi Key: GZZZSOOGQLOEOB-UHFFFAOYSA-N

InChi Code: InChI=1S/C9H9Cl2NO2/c1-12(9(14)8(10)11)6-2-4-7(13)5-3-6/h2-5,8,13H,1H3

SMILES Code: O=C(N(C1=CC=C(O)C=C1)C)C(Cl)Cl

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO, not in water

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 234.08 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.
=
x
x
g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Dilution Calculator

Calculate the dilution required to prepare a stock solution.
x
=
x

1: Tiwari V, Verma S, Verma SK, Dangi JS. Enhancement of site specific delivery of diloxanide furoate as an antiamoebic drug. Eur J Pharm Sci. 2016 Mar 4. pii: S0928-0987(16)30054-9. doi: 10.1016/j.ejps.2016.03.001. [Epub ahead of print] PubMed PMID: 26952868.

2: Elkhoudary MM, Abdel Salam RA, Hadad GM. Comparative artificial neural network and partial least squares models for analysis of Metronidazole, Diloxanide, Spiramycin and Cliquinol in pharmaceutical preparations. Spectrochim Acta A Mol Biomol Spectrosc. 2014 Sep 15;130:222-9. doi: 10.1016/j.saa.2014.04.002. Epub 2014 Apr 13. PubMed PMID: 24792195.

3: Issa MM, Nejem RM, Abu Shanab AM, Shaat NT. Kinetic spectrophotometric H-point standard addition method for the simultaneous determination of diloxanide furoate and metronidazole in binary mixtures and biological fluids. Spectrochim Acta A Mol Biomol Spectrosc. 2013 Oct;114:592-8. doi: 10.1016/j.saa.2013.05.062. Epub 2013 Jun 5. PubMed PMID: 23807049.

4: Mabrouk M, El-Fatatry H, Hewala I, Emam E. Development and application of a novel, dual-mode gradient, stability-indicating HPLC-DAD method for the simultaneous determination and purity assessment of mebeverine hydrochloride, diloxanide furoate and their corresponding major degradation products in combination with some gastrointestinal drugs in the form of oral doses. J Pharm Biomed Anal. 2013 Sep;83:249-59. doi: 10.1016/j.jpba.2013.04.013. Epub 2013 Apr 19. PubMed PMID: 23770637.

5: Abbas SS, Wagieh NE, Abdelkawy M, Abdelrahman MM. Simultaneous determination of diloxanide furoate and metronidazole in presence of diloxanide furoate degradation products. J AOAC Int. 2011 Sep-Oct;94(5):1427-39. PubMed PMID: 22165007.

6: Anande NM, Jain SK, Jain NK. Con-A conjugated mucoadhesive microspheres for the colonic delivery of diloxanide furoate. Int J Pharm. 2008 Jul 9;359(1-2):182-9. doi: 10.1016/j.ijpharm.2008.04.009. Epub 2008 Apr 12. PubMed PMID: 18486369.

7: Mishal A, Sober D. Stability indicating reversed-phase liquid chromatographic determination of metronidazole benzoate and diloxanide furoate as bulk drug and in suspension dosage form. J Pharm Biomed Anal. 2005 Sep 15;39(3-4):819-23. PubMed PMID: 16055294.

8: Monteiro JB, Chiaradia LD, Brandão TA, Magro JD, Yunes RA. Enzymatic hydrolysis of diloxanide furoate in the presence of beta-cyclodextrin and its methylated derivatives. Int J Pharm. 2003 Nov 28;267(1-2):93-100. PubMed PMID: 14602387.

9: Hasan NY, Elkawy MA, Elzeany BE, Wagieh NE. Stability indicating methods for the determination of diloxanide furoate. J Pharm Biomed Anal. 2002 Apr 15;28(2):187-97. PubMed PMID: 11929661.

10: Castro Hermida JA, Ares-Mazás ME, Nieto Reyes L, Otero Espinar F, Blanco Méndez J. Inhibition of Cryptosporidium infection in mice treated with a cyclodextrin inclusion complex with diloxanide furoate. Parasitol Res. 2001 Jun;87(6):449-52. PubMed PMID: 11411943.

11: Sengupta M, Sengupta O. Correlation of biological activity (therapeutic and toxic) with solvochromic properties of metronidazole, emetine hydrochloride and diloxanide furoate. Indian J Biochem Biophys. 1995 Oct;32(5):302-7. PubMed PMID: 8713754.

12: Thorén K, Håkansson C, Bergström T, Johanisson G, Norkrans G. Treatment of asymptomatic amebiasis in homosexual men. Clinical trials with metronidazole, tinidazole, and diloxanide furoate. Sex Transm Dis. 1990 Apr-Jun;17(2):72-4. PubMed PMID: 2193407.

13: Osisanya JO. Comparative in vitro activity of mefloquine, diloxanide furoate and other conventionally used amoebicides against entamoeba histolytica. East Afr Med J. 1986 Apr;63(4):263-8. PubMed PMID: 2874011.

14: Powell SJ, Stewart-Wynne EJ, Elsdon-Dew R. Metronidazole combined with diloxanide furoate in amoebic liver abscess. Ann Trop Med Parasitol. 1973 Sep;67(3):367-8. PubMed PMID: 4357547.

15: Nnochiri E. Oral chemotherapy in amoebic dysentery--potentiating effect of chloroquine on the action of diloxanide furoate. J Trop Med Hyg. 1967 Sep;70(9):224-8. PubMed PMID: 6058250.

16: Botero D. Treatment of intestinal amoebiasis with diloxanide furoate, tetracycline and chloroquine. Trans R Soc Trop Med Hyg. 1967;61(6):769-73. PubMed PMID: 6080665.

17: Huggins D. [Treatment of amebiasis. Results obtained with diloxanide furoate]. Rev Inst Med Trop Sao Paulo. 1965 Mar-Apr;7(2):110-1. Portuguese. PubMed PMID: 5318288.

18: GEOFFROY H, ZNIBER A, SCHWARTZ R, KOUHEN C, ABITBOL E. [A new amebicide: diloxanide furoate]. Maroc Med. 1962 Jan;41:98-104. French. PubMed PMID: 13897741.

19: Pai PN, Rao GK, Srinivas B, Puranik S. RPLC Determination of Tinidazole and Diloxanide Furoate in Tablets. Indian J Pharm Sci. 2008 Sep;70(5):670-2. doi: 10.4103/0250-474X.45415. PubMed PMID: 21394273; PubMed Central PMCID: PMC3038301.

20: Gadkariem EA, Belal F, Abounassif MA, El-Obeid HA, E E Ibrahim K. Stability studies on diloxanide furoate: effect of pH, temperature, gastric and intestinal fluids. Farmaco. 2004 Apr;59(4):323-9. PubMed PMID: 15081350.