WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H208445
CAS#: unknown
Description: Small protein B (SmpB) is a requisite component of the transfer messenger RNA (tmRNA)-mediated bacterial translational quality control system known as trans-translation. The initial binding of tmRNA and its subsequent accommodation into the ribosomal A-site are activities intimately linked to SmpB protein function.
Hodoodo Cat#: H208445
Name: S-FMPB
CAS#: unknown
Chemical Formula: C24H25FN2O4
Exact Mass: 424.18
Molecular Weight: 424.470
Elemental Analysis: C, 67.91; H, 5.94; F, 4.48; N, 6.60; O, 15.08
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Synonym: SFMPB
IUPAC/Chemical Name: 1-(4-Fluorophenyl)-3-methyl-1H-pyrazol-5-yl (S)-2-(1-acetoxypentyl)benzoate
InChi Key: CUIMOBFQBZQYMB-QFIPXVFZSA-N
InChi Code: InChI=1S/C24H25FN2O4/c1-4-5-10-22(30-17(3)28)20-8-6-7-9-21(20)24(29)31-23-15-16(2)26-27(23)19-13-11-18(25)12-14-19/h6-9,11-15,22H,4-5,10H2,1-3H3/t22-/m0/s1
SMILES Code: O=C(OC1=CC(C)=NN1C2=CC=C(F)C=C2)C3=CC=CC=C3[C@@H](OC(C)=O)CCCC
Appearance: To be determined
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: To be determined
Shelf Life: >2 years if stored properly
Drug Formulation: To be determined
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info:
| Biological target: | |
| In vitro activity: | |
| In vivo activity: |
The following data is based on the product molecular weight 424.47 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
| Formulation protocol: | |
| In vitro protocol: | |
| In vivo protocol: |
1: Felden B, Gillet R. SmpB as the handyman of tmRNA during trans-translation. RNA Biol. 2011 May-Jun;8(3):440-9. doi: 10.4161/rna.8.3.15387. Epub 2011 May 1. PMID: 21558793.
2: Sundermeier TR, Karzai AW. Functional SmpB-ribosome interactions require tmRNA. J Biol Chem. 2007 Nov 30;282(48):34779-86. doi: 10.1074/jbc.M707256200. Epub 2007 Oct 2. PMID: 17911096; PMCID: PMC2570653.
3: Hanawa-Suetsugu K, Takagi M, Inokuchi H, Himeno H, Muto A. SmpB functions in various steps of trans-translation. Nucleic Acids Res. 2002 Apr 1;30(7):1620-9. doi: 10.1093/nar/30.7.1620. PMID: 11917023; PMCID: PMC101834.
4: Ivanova N, Pavlov MY, Bouakaz E, Ehrenberg M, Schiavone LH. Mapping the interaction of SmpB with ribosomes by footprinting of ribosomal RNA. Nucleic Acids Res. 2005 Jun 21;33(11):3529-39. doi: 10.1093/nar/gki666. PMID: 15972795; PMCID: PMC1156966.
5: Cheng K, Ivanova N, Scheres SH, Pavlov MY, Carazo JM, Hebert H, Ehrenberg M, Lindahl M. tmRNA.SmpB complex mimics native aminoacyl-tRNAs in the A site of stalled ribosomes. J Struct Biol. 2010 Mar;169(3):342-8. doi: 10.1016/j.jsb.2009.10.015. Epub 2009 Oct 31. PMID: 19883769.
6: Zhang Y, Liu Z, Tang Y, Ma X, Tang H, Li H, Liu Z. Cbl upregulates cysH for hydrogen sulfide production in Aeromonas veronii. PeerJ. 2021 Sep 9;9:e12058. doi: 10.7717/peerj.12058. PMID: 34589297; PMCID: PMC8435198.
7: Sundermeier TR, Dulebohn DP, Cho HJ, Karzai AW. A previously uncharacterized role for small protein B (SmpB) in transfer messenger RNA-mediated trans- translation. Proc Natl Acad Sci U S A. 2005 Feb 15;102(7):2316-21. doi: 10.1073/pnas.0409694102. Epub 2005 Feb 7. PMID: 15699355; PMCID: PMC549014.
8: Yang C, Glover JR. The SmpB-tmRNA tagging system plays important roles in Streptomyces coelicolor growth and development. PLoS One. 2009;4(2):e4459. doi: 10.1371/journal.pone.0004459. Epub 2009 Feb 12. PMID: 19212432; PMCID: PMC2635970.
9: Campos-Silva R, D'Urso G, Delalande O, Giudice E, Macedo AJ, Gillet R. Trans-Translation Is an Appealing Target for the Development of New Antimicrobial Compounds. Microorganisms. 2021 Dec 21;10(1):3. doi: 10.3390/microorganisms10010003. PMID: 35056452; PMCID: PMC8778911.
10: Moreira RN, Domingues S, Viegas SC, Amblar M, Arraiano CM. Synergies between RNA degradation and trans-translation in Streptococcus pneumoniae: cross regulation and co-transcription of RNase R and SmpB. BMC Microbiol. 2012 Nov 20;12:268. doi: 10.1186/1471-2180-12-268. PMID: 23167513; PMCID: PMC3534368.
