WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H526809
CAS#: 18711-16-5
Description: NS309 is a nonselective activator of small- and intermediate-conductance Ca2+-activated K+ (SK/KCa2 and IK/KCa3.1) channels (EC50s range from 0.12-1.2 µM for SK channels and 10-90 nM for IK channels). NS309 decreases rat detrusor smooth muscle membrane potential and phasic contractions by activating SK3 channels. NS309 restores EDHF-type relaxation in mesenteric small arteries from type 2 diabetic ZDF rats.
Hodoodo Cat#: H526809
Name: NS309
CAS#: 18711-16-5
Chemical Formula: C8H4Cl2N2O2
Exact Mass: 229.97
Molecular Weight: 231.032
Elemental Analysis: C, 41.59; H, 1.75; Cl, 30.69; N, 12.13; O, 13.85
Synonym: NS309; NS-309; NS 309.
IUPAC/Chemical Name: (Z)-6,7-dichloro-3-(hydroxyimino)indolin-2-one
InChi Key: CVOUSAVHMDXCKG-UHFFFAOYSA-N
InChi Code: InChI=1S/C8H4Cl2N2O2/c9-4-2-1-3-6(5(4)10)11-8(13)7(3)12-14/h1-2,14H,(H,11,12,13)
SMILES Code: O=C1/C(C2=CC=C(Cl)C(Cl)=C2N1)=N\O
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO
Shelf Life: >2 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info:
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| In vitro activity: | |
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The following data is based on the product molecular weight 231.03 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
| Formulation protocol: | |
| In vitro protocol: | |
| In vivo protocol: |
1: Tajbakhsh N, Sokoya EM. Compromised endothelium-dependent hyperpolarization-mediated dilations can be rescued by NS309 in obese Zucker rats. Microcirculation. 2014 Nov;21(8):747-53. doi: 10.1111/micc.12157. PubMed PMID: 25047389.
2: Parajuli SP, Hristov KL, Soder RP, Kellett WF, Petkov GV. NS309 decreases rat detrusor smooth muscle membrane potential and phasic contractions by activating SK3 channels. Br J Pharmacol. 2013 Apr;168(7):1611-25. doi: 10.1111/bph.12049. PubMed PMID: 23145946; PubMed Central PMCID: PMC3605870.
3: Kroigaard C, Dalsgaard T, Nielsen G, Laursen BE, Pilegaard H, Köhler R, Simonsen U. Activation of endothelial and epithelial K(Ca) 2.3 calcium-activated potassium channels by NS309 relaxes human small pulmonary arteries and bronchioles. Br J Pharmacol. 2012 Sep;167(1):37-47. doi: 10.1111/j.1476-5381.2012.01986.x. PubMed PMID: 22506557; PubMed Central PMCID: PMC3448912.
4: Seear RV, Lew VL. IKCa agonist (NS309)-elicited all-or-none dehydration response of human red blood cells is cell-age dependent. Cell Calcium. 2011 Nov;50(5):444-8. doi: 10.1016/j.ceca.2011.07.005. Epub 2011 Sep 21. PubMed PMID: 21937109.
5: Brøndum E, Kold-Petersen H, Simonsen U, Aalkjaer C. NS309 restores EDHF-type relaxation in mesenteric small arteries from type 2 diabetic ZDF rats. Br J Pharmacol. 2010 Jan;159(1):154-65. doi: 10.1111/j.1476-5381.2009.00525.x. Epub 2009 Dec 10. PubMed PMID: 20015296; PubMed Central PMCID: PMC2823361.
6: Pandita RK, Rønn LC, Jensen BS, Andersson KE. Urodynamic effects of intravesical administration of the new small/intermediate conductance calcium activated potassium channel activator NS309 in freely moving, conscious rats. J Urol. 2006 Sep;176(3):1220-4. PubMed PMID: 16890729.
7: Strøbaek D, Teuber L, Jørgensen TD, Ahring PK, Kjaer K, Hansen RS, Olesen SP, Christophersen P, Skaaning-Jensen B. Activation of human IK and SK Ca2+ -activated K+ channels by NS309 (6,7-dichloro-1H-indole-2,3-dione 3-oxime). Biochim Biophys Acta. 2004 Oct 11;1665(1-2):1-5. PubMed PMID: 15471565.
