DCFH
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Hodoodo CAT#: H556201

CAS#: 106070-31-9

Description: DCFH, also known as 2',7'-dichlorodihydrofluorescein, is a fluororescent agent. It is a key chemcial for the dichlorofluorescin (DCFH) oxidation assay. Fluorogenic probes such as 2',7'-dichlorofluorescin (DCFH) have been extensively used to detect oxidative events and to measure antioxidant capacity.

Chemical Structure

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DCFH
CAS# 106070-31-9
Instruction

Theoretical Analysis

Hodoodo Cat#: H556201
Name: DCFH
CAS#: 106070-31-9
Chemical Formula: C20H12Cl2O5
Exact Mass: 402.01
Molecular Weight: 403.211
Elemental Analysis: C, 59.58; H, 3.00; Cl, 17.58; O, 19.84

Price and Availability

Size Price Availability Quantity
200mg USD 1350 2 Weeks
500mg USD 2650 2 Weeks
1g USD 3650 2 Weeks
2g USD 6250 2 Weeks
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Synonym: DCFH, 2',7'-dichlorodihydrofluorescein;

IUPAC/Chemical Name: 2-(2,7-Dichloro-3,6-dihydroxy-9H-xanthen-9-yl)benzoic acid

InChi Key: XDFNWJDGWJVGGN-UHFFFAOYSA-N

InChi Code: InChI=1S/C20H12Cl2O5/c21-13-5-11-17(7-15(13)23)27-18-8-16(24)14(22)6-12(18)19(11)9-3-1-2-4-10(9)20(25)26/h1-8,19,23-24H,(H,25,26)

SMILES Code: O=C(O)C1=CC=CC=C1C2C3=C(OC4=C2C=C(Cl)C(O)=C4)C=C(O)C(Cl)=C3

Appearance: To be determined

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: To be determined

Shelf Life: >2 years if stored properly

Drug Formulation: To be determined

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 403.21 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Benedetti S, Catalani S, De Stefani S, Primiterra M, Fraternale A, Palma F, Palini S. A microplate-based DCFH-DA assay for the evaluation of oxidative stress in whole semen. Heliyon. 2022 Sep 15;8(9):e10642. doi: 10.1016/j.heliyon.2022.e10642. PMID: 36158085; PMCID: PMC9489972.


2: de Haan LR, Reiniers MJ, Reeskamp LF, Belkouz A, Ao L, Cheng S, Ding B, van Golen RF, Heger M. Experimental Conditions That Influence the Utility of 2'7'-Dichlorodihydrofluorescein Diacetate (DCFH2-DA) as a Fluorogenic Biosensor for Mitochondrial Redox Status. Antioxidants (Basel). 2022 Jul 22;11(8):1424. doi: 10.3390/antiox11081424. PMID: 35892626; PMCID: PMC9329753.


3: Boyles M, Murphy F, Mueller W, Wohlleben W, Jacobsen NR, Braakhuis H, Giusti A, Stone V. Development of a standard operating procedure for the DCFH2-DA acellular assessment of reactive oxygen species produced by nanomaterials. Toxicol Mech Methods. 2022 Jul;32(6):439-452. doi: 10.1080/15376516.2022.2029656. Epub 2022 Jan 28. PMID: 35086424.


4: Jovanovic MV, Savic JZ, Salimi F, Stevanovic S, Brown RA, Jovasevic- Stojanovic M, Manojlovic D, Bartonova A, Bottle S, Ristovski ZD. Measurements of Oxidative Potential of Particulate Matter at Belgrade Tunnel; Comparison of BPEAnit, DTT and DCFH Assays. Int J Environ Res Public Health. 2019 Dec 5;16(24):4906. doi: 10.3390/ijerph16244906. PMID: 31817307; PMCID: PMC6950172.


5: Rajneesh, Pathak J, Chatterjee A, Singh SP, Sinha RP. Detection of Reactive Oxygen Species (ROS) in Cyanobacteria Using the Oxidant-sensing Probe 2',7'-Dichlorodihydrofluorescein Diacetate (DCFH-DA). Bio Protoc. 2017 Sep 5;7(17):e2545. doi: 10.21769/BioProtoc.2545. PMID: 34541194; PMCID: PMC8413551.


6: Aranda A, Sequedo L, Tolosa L, Quintas G, Burello E, Castell JV, Gombau L. Dichloro-dihydro-fluorescein diacetate (DCFH-DA) assay: a quantitative method for oxidative stress assessment of nanoparticle-treated cells. Toxicol In Vitro. 2013 Mar;27(2):954-63. doi: 10.1016/j.tiv.2013.01.016. Epub 2013 Jan 26. PMID: 23357416.


7: Pal AK, Bello D, Budhlall B, Rogers E, Milton DK. Screening for Oxidative Stress Elicited by Engineered Nanomaterials: Evaluation of Acellular DCFH Assay. Dose Response. 2012;10(3):308-30. doi: 10.2203/dose-response.10-036.Pal. Epub 2011 May 26. PMID: 22942866; PMCID: PMC3430394.


8: Laggner H, Hermann M, Gmeiner BM, Kapiotis S. Cu2+ and Cu+ bathocuproine disulfonate complexes promote the oxidation of the ROS-detecting compound dichlorofluorescin (DCFH). Anal Bioanal Chem. 2006 Jul;385(5):959-61. doi: 10.1007/s00216-006-0498-y. Epub 2006 May 20. PMID: 16791578.


9: Afri M, Frimer AA, Cohen Y. Active oxygen chemistry within the liposomal bilayer. Part IV: Locating 2',7'-dichlorofluorescein (DCF), 2',7'-dichlorodihydrofluorescein (DCFH) and 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA) in the lipid bilayer. Chem Phys Lipids. 2004 Aug;131(1):123-33. doi: 10.1016/j.chemphyslip.2004.04.006. PMID: 15210370.


10: Yoshida Y, Shimakawa S, Itoh N, Niki E. Action of DCFH and BODIPY as a probe for radical oxidation in hydrophilic and lipophilic domain. Free Radic Res. 2003 Aug;37(8):861-72. doi: 10.1080/1071576031000148736. PMID: 14567446.


11: Caldefie-Chézet F, Walrand S, Moinard C, Tridon A, Chassagne J, Vasson MP. Is the neutrophil reactive oxygen species production measured by luminol and lucigenin chemiluminescence intra or extracellular? Comparison with DCFH-DA flow cytometry and cytochrome c reduction. Clin Chim Acta. 2002 May 7;319(1):9-17. doi: 10.1016/s0009-8981(02)00015-3. PMID: 11922918.


12: Vuorte J, Jansson SE, Repo H. Evaluation of red blood cell lysing solutions in the study of neutrophil oxidative burst by the DCFH assay. Cytometry. 2001 Apr 1;43(4):290-6. PMID: 11260596.


13: Imrich A, Kobzik L. Flow cytometric analysis of macrophage oxidative metabolism using DCFH. Methods Mol Biol. 1998;91:97-108. doi: 10.1385/0-89603-354-6:97. PMID: 9664485.


14: Doi H, Fukunaga Y. [Flow cytometric measurement of DCFH-oxidation of neutrophils in peripheral whole blood, isolated leukocytes and cerebrospinal fluid cells]. Nihon Ika Daigaku Zasshi. 1996 Jun;63(3):192-201. Japanese. doi: 10.1272/jnms1923.63.192. PMID: 8707920.