WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H130340
CAS#: 1313211-52-7
Description: PTAD-Azide is a crosslinking reagent used in the formation of hydrogels, for protein modifications, and in e-Y-CLICK chemistry. PTAD-Azide has the ability to introduce PEG chains onto proteins that have exposed tyrosine residues on the surface.
Hodoodo Cat#: H130340
Name: PTAD-Azide
CAS#: 1313211-52-7
Chemical Formula: C10H10N6O3
Exact Mass: 262.08
Molecular Weight: 262.230
Elemental Analysis: C, 45.80; H, 3.84; N, 32.05; O, 18.30
Synonym: PTAD-Azide
IUPAC/Chemical Name: 4-(4-(2-azidoethoxy)phenyl)-1,2,4-triazolidine-3,5-dione
InChi Key: MHGMHPVYCVQIET-UHFFFAOYSA-N
InChi Code: InChI=1S/C10H10N6O3/c11-15-12-5-6-19-8-3-1-7(2-4-8)16-9(17)13-14-10(16)18/h1-4H,5-6H2,(H,13,17)(H,14,18)
SMILES Code: O=C(N1C2=CC=C(OCCN=[N+]=[N-])C=C2)NNC1=O
Appearance: To be determined
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: To be determined
Shelf Life: >2 years if stored properly
Drug Formulation: To be determined
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info:
Biological target: | |
In vitro activity: | |
In vivo activity: |
The following data is based on the product molecular weight 262.23 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
---|---|---|---|
1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
Formulation protocol: | |
In vitro protocol: | |
In vivo protocol: |
1. Madl CM, Heilshorn SC. Tyrosine-Selective Functionalization for Bio-Orthogonal Cross-Linking of Engineered Protein Hydrogels. Bioconjug Chem. 2017 Mar 15;28(3):724-730. doi: 10.1021/acs.bioconjchem.6b00720. Epub 2017 Feb 2. PMID: 28151642; PMCID: PMC7274545.
2. Moinpour M, Barker NK, Guzman LE, Jewett JC, Langlais PR, Schwartz JC. Discriminating changes in protein structure using tyrosine conjugation. Protein Sci. 2020 Aug;29(8):1784-1793. doi: 10.1002/pro.3897. Epub 2020 Jul 6. PMID: 32483864; PMCID: PMC7380675.