WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H130343
CAS#: N/A
Description: TCO-amine HCl salt is an agent with applications in biological labeling and imaging by reacting with 1; 2; 4; 5-tetrazines for ligation.
Hodoodo Cat#: H130343
Name: TCO-amine HCl salt
CAS#: N/A
Chemical Formula: C12H23ClN2O2
Exact Mass: 0.00
Molecular Weight: 262.780
Elemental Analysis: C, 54.85; H, 8.82; Cl, 13.49; N, 10.66; O, 12.18
Synonym: TCO-amine HCl salt; trans-Cyclooctene-amine hydrochloride salt; TCO-NH2, HCl salt
IUPAC/Chemical Name: (E)-cyclooct-4-en-1-yl (3-aminopropyl)carbamate hydrochloride
InChi Key: GIYQQURLGCGUKK-TYYBGVCCSA-N
InChi Code: InChI=1S/C12H22N2O2.ClH/c13-9-6-10-14-12(15)16-11-7-4-2-1-3-5-8-11;/h1-2,11H,3-10,13H2,(H,14,15);1H/b2-1+;
SMILES Code: O=C(OC1CCC/C=C/CC1)NCCCN.[H]Cl
Appearance: To be determined
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: To be determined
Shelf Life: >2 years if stored properly
Drug Formulation: To be determined
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info:
Biological target: | |
In vitro activity: | |
In vivo activity: |
The following data is based on the product molecular weight 262.78 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
---|---|---|---|
1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
Formulation protocol: | |
In vitro protocol: | |
In vivo protocol: |
1. Karver MR, Weissleder R, Hilderbrand SA. Synthesis and evaluation of a series of 1,2,4,5-tetrazines for bioorthogonal conjugation. Bioconjug Chem. 2011 Nov 16;22(11):2263-70. doi: 10.1021/bc200295y. Epub 2011 Oct 24. PMID: 21950520; PMCID: PMC3254257.
2. Blackman ML, Royzen M, Fox JM. Tetrazine ligation: fast bioconjugation based on inverse-electron-demand Diels-Alder reactivity. J Am Chem Soc. 2008 Oct 15;130(41):13518-9. doi: 10.1021/ja8053805. Epub 2008 Sep 18. PMID: 18798613; PMCID: PMC2653060.
3. Devaraj NK, Weissleder R, Hilderbrand SA. Tetrazine-based cycloadditions: application to pretargeted live cell imaging. Bioconjug Chem. 2008 Dec;19(12):2297-9. doi: 10.1021/bc8004446. PMID: 19053305; PMCID: PMC2677645.