WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H328812
CAS#: 518-47-8(sodium)
Description: Fluorescein Sodium, also known as Fluorescite and VST-1001, is a phthalic indicator dye that is used therapeutically as a diagnostic aid in corneal injuries and corneal trauma.
Hodoodo Cat#: H328812
Name: Fluorescein Sodium
CAS#: 518-47-8(sodium)
Chemical Formula: C20H10Na2O5
Exact Mass: 0.00
Molecular Weight: 376.275
Elemental Analysis: C, 63.84; H, 2.68; Na, 12.22; O, 21.26
Related CAS #: 518-47-8(sodium) 2321-07-5(free base)
Synonym: Fluorescein Sodium; VST-1001; VST 1001; VST1001; Fluorescite
IUPAC/Chemical Name: sodium 3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-3',6'-bis(olate)
InChi Key: RGPLGPBQJOQFJS-UHFFFAOYSA-L
InChi Code: InChI=1S/C20H12O5.2Na/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;;/h1-10,21-22H;;/q;2*+1/p-2
SMILES Code: O=C1OC2(C3=C(OC4=C2C=CC([O-])=C4)C=C([O-])C=C3)C5=C1C=CC=C5.[Na+].[Na+]
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO
Shelf Life: >2 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info: Related CAS# 518-47-8(Fluorescein Sodium) 2321-07-5(Fluorescein)
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The following data is based on the product molecular weight 376.27 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
| Formulation protocol: | |
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| In vivo protocol: |
1: Eom Y, Lee JS, Keun Lee H, Myung Kim H, Suk Song J. Comparison of conjunctival staining between lissamine green and yellow filtered fluorescein sodium. Can J Ophthalmol. 2015 Aug;50(4):273-7. doi: 10.1016/j.jcjo.2015.05.007. PubMed PMID: 26257220.
2: Ayestaray B, Bekara F. Fluorescein sodium fluorescence microscope-integrated lymphangiography for lymphatic supermicrosurgery. Microsurgery. 2015 Jul;35(5):407-10. doi: 10.1002/micr.22368. Epub 2015 Jan 6. PubMed PMID: 25565342.
3: Ung TH, Kellner C, Neira JA, Wang SH, D'Amico R, Faust PL, Canoll P, Feldstein NA, Bruce JN. The use of fluorescein sodium in the biopsy and gross-total resection of a tectal plate glioma. J Neurosurg Pediatr. 2015 Dec;16(6):732-5. doi: 10.3171/2015.5.PEDS15142. Epub 2015 Sep 25. PubMed PMID: 26407010.
4: Bhopate DP, Mahajan PG, Garadkar KM, Kolekar GB, Patil SR. Polyvinyl pyrrolidone capped fluorescent anthracene nanoparticles for sensing fluorescein sodium in aqueous solution and analytical application for ophthalmic samples. Luminescence. 2015 Nov;30(7):1055-63. doi: 10.1002/bio.2858. Epub 2015 Mar 3. PubMed PMID: 25736374.
5: Schebesch KM, Hoehne J, Hohenberger C, Acerbi F, Broggi M, Proescholdt M, Wendl C, Riemenschneider MJ, Brawanski A. Fluorescein sodium-guided surgery in cerebral lymphoma. Clin Neurol Neurosurg. 2015 Dec;139:125-8. doi: 10.1016/j.clineuro.2015.09.015. Epub 2015 Sep 26. PubMed PMID: 26432995.
6: Zhang YL, Bai L, Li Z, Ji R, Li CQ, Zuo XL, Yin YF, Du JX, Zhai ZZ, Gao XZ, Li YQ. A lower dose of fluorescein sodium is more suitable for confocal laser endomicroscopy: a feasibility study. Gastrointest Endosc. 2016 May 14. pii: S0016-5107(16)30157-2. doi: 10.1016/j.gie.2016.05.011. [Epub ahead of print] PubMed PMID: 27189657.
7: Martirosyan NL, Eschbacher JM, Kalani MY, Turner JD, Belykh E, Spetzler RF, Nakaji P, Preul MC. Prospective evaluation of the utility of intraoperative confocal laser endomicroscopy in patients with brain neoplasms using fluorescein sodium: experience with 74 cases. Neurosurg Focus. 2016 Mar;40(3):E11. doi: 10.3171/2016.1.FOCUS15559. PubMed PMID: 26926051.
8: Schebesch KM, Hoehne J, Hohenberger C, Proescholdt M, Riemenschneider MJ, Wendl C, Brawanski A. Fluorescein sodium-guided resection of cerebral metastases—experience with the first 30 patients. Acta Neurochir (Wien). 2015 Jun;157(6):899-904. doi: 10.1007/s00701-015-2395-7. Epub 2015 Apr 1. PubMed PMID: 25824557.
9: Liang Q, Duan L, Zhuang Z, Zhao B, Liu Y, Wang S, Yang F, Liu S, Li G. [Effect of capsaicin on intestinal permeation of P-glycoprotein substrate rhodamine 123 and fluorescein sodium in rats]. Nan Fang Yi Ke Da Xue Xue Bao. 2015 May;35(5):724-7, 732. Chinese. PubMed PMID: 26018271.
10: Ichikawa T, Suzuki K, Watanabe Y. Intra-arterial fluorescence angiography with injection of fluorescein sodium from the superficial temporal artery during aneurysm surgery: technical notes. Neurol Med Chir (Tokyo). 2014 Jun 17;54(6):490-6. Epub 2014 Jan 28. PubMed PMID: 24477067; PubMed Central PMCID: PMC4533452.
11: Rey-Dios R, Hattab EM, Cohen-Gadol AA. Use of intraoperative fluorescein sodium fluorescence to improve the accuracy of tissue diagnosis during stereotactic needle biopsy of high-grade gliomas. Acta Neurochir (Wien). 2014 Jun;156(6):1071-5; discussion 1075. doi: 10.1007/s00701-014-2097-6. Epub 2014 Apr 27. PubMed PMID: 24770732.
12: Liu M, Jia M, Pan H, Li L, Chang M, Ren H, Argoul F, Zhang S, Xu J. Instrument response standard in time-resolved fluorescence spectroscopy at visible wavelength: quenched fluorescein sodium. Appl Spectrosc. 2014;68(5):577-83. doi: 10.1366/13-07236. PubMed PMID: 25014602.
13: Patik I, Kovacsics D, Német O, Gera M, Várady G, Stieger B, Hagenbuch B, Szakács G, Özvegy-Laczka C. Functional expression of the 11 human Organic Anion Transporting Polypeptides in insect cells reveals that sodium fluorescein is a general OATP substrate. Biochem Pharmacol. 2015 Dec 15;98(4):649-58. doi: 10.1016/j.bcp.2015.09.015. Epub 2015 Sep 28. PubMed PMID: 26415544; PubMed Central PMCID: PMC4959548.
14: Moein S, Mohajer R. Fluorescein sodium as a contrast agent for colposcopy. Int J Gynaecol Obstet. 2016 Feb;132(2):238-9. doi: 10.1016/j.ijgo.2015.08.002. Epub 2015 Nov 26. PubMed PMID: 26857076.
15: Diaz RJ, Dios RR, Hattab EM, Burrell K, Rakopoulos P, Sabha N, Hawkins C, Zadeh G, Rutka JT, Cohen-Gadol AA. Study of the biodistribution of fluorescein in glioma-infiltrated mouse brain and histopathological correlation of intraoperative findings in high-grade gliomas resected under fluorescein fluorescence guidance. J Neurosurg. 2015 Jun;122(6):1360-9. doi: 10.3171/2015.2.JNS132507. Epub 2015 Apr 3. PubMed PMID: 25839919.
