WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H530621
CAS#: 957011-15-3
Description: HBF-0259 is a novel inhibitors of hepatitis B virus surface antigen (HBsAg) secretion. HBF-0259 was identified to inhibit HBsAg with an EC50 of 11.3 μM and a CC50 value of >50 μM in HepG2.2.15 cells. HBF-0259 had a 50% effective concentration of approximately 1.5 microM in a secondary, HBV-expressing cell line, with a concentration that exhibited 50% cytotoxicity of >50 microM. The equilibrium concentration of HBF-0259 in aqueous solution at physiological pH was 15 to 16 microM; the selective index was thus >9.
Hodoodo Cat#: H530621
Name: HBF-0259
CAS#: 957011-15-3
Chemical Formula: C16H12Cl2FN5
Exact Mass: 363.05
Molecular Weight: 364.205
Elemental Analysis: C, 52.77; H, 3.32; Cl, 19.47; F, 5.22; N, 19.23
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Synonym: HBF-0259; HBF 0259; HBF0259.
IUPAC/Chemical Name: 7-(2-Chloro-6-fluorophenyl)-5-(4-chlorophenyl)-1,5,6,7-tetrahydrotetrazolo[1,5-a]pyrimidine
InChi Key: BRBZPYDLUUWBCD-UHFFFAOYSA-N
InChi Code: InChI=1S/C16H12Cl2FN5/c17-10-6-4-9(5-7-10)13-8-14(24-16(20-13)21-22-23-24)15-11(18)2-1-3-12(15)19/h1-7,13-14H,8H2,(H,20,21,23)
SMILES Code: ClC1=CC=C(C2N=C3N(NN=N3)C(C4=C(F)C=CC=C4Cl)C2)C=C1
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO
Shelf Life: >2 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info: The high levels of hepatitis B virus (HBV) surface antigen (HBsAg)-bearing subviral particles in the serum of chronically infected individuals play an important role in suppressing HBV-specific immune response and are only mildly affected by the current small molecule therapies.
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The following data is based on the product molecular weight 364.21 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
| Formulation protocol: | |
| In vitro protocol: | |
| In vivo protocol: |
1: Yu W, Goddard C, Clearfield E, Mills C, Xiao T, Guo H, Morrey JD, Motter NE,
Zhao K, Block TM, Cuconati A, Xu X. Design, synthesis, and biological evaluation
of triazolo-pyrimidine derivatives as novel inhibitors of hepatitis B virus
surface antigen (HBsAg) secretion. J Med Chem. 2011 Aug 25;54(16):5660-70. doi:
10.1021/jm200696v. Epub 2011 Aug 2. PubMed PMID: 21786803; PubMed Central PMCID:
PMC3158247.
2: Dougherty AM, Guo H, Westby G, Liu Y, Simsek E, Guo JT, Mehta A, Norton P, Gu
B, Block T, Cuconati A. A substituted tetrahydro-tetrazolo-pyrimidine is a
specific and novel inhibitor of hepatitis B virus surface antigen secretion.
Antimicrob Agents Chemother. 2007 Dec;51(12):4427-37. Epub 2007 Sep 17. PubMed
PMID: 17875990; PubMed Central PMCID: PMC2167973.
