B02
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Hodoodo CAT#: H530630

CAS#: 1290541-46-6

Description: B02, also known as RAD51-IN-02, is a RAD51 inhibitor. B02 can enhance DOX sensitivity of MM cells. Combining low-toxicity doses of DOX and B02 resulted in significant synthetic lethality, observed as increased apoptosis and reduced viability compared to either agent alone, or to the product of their individual effects.

Chemical Structure

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B02
CAS# 1290541-46-6
Instruction

Theoretical Analysis

Hodoodo Cat#: H530630
Name: B02
CAS#: 1290541-46-6
Chemical Formula: C22H17N3O
Exact Mass: 339.14
Molecular Weight: 339.398
Elemental Analysis: C, 77.86; H, 5.05; N, 12.38; O, 4.71

Price and Availability

Size Price Availability Quantity
100mg USD 950 2 Weeks
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Synonym: B02; B-02; B 02; RAD51-IN-02

IUPAC/Chemical Name: 3-Benzyl-2-[(E)-2-(3-pyridinyl)ethenyl]-4(3H)-quinazolinone

InChi Key: GEKDQXSPTHHANP-OUKQBFOZSA-N

InChi Code: InChI=1S/C22H17N3O/c26-22-19-10-4-5-11-20(19)24-21(13-12-17-9-6-14-23-15-17)25(22)16-18-7-2-1-3-8-18/h1-15H,16H2/b13-12+

SMILES Code: O=C1N(CC2=CC=CC=C2)C(/C=C/C3=CC=CN=C3)=NC4=C1C=CC=C4

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info: high RAD51 expression in vivo is associated with chemoresistance and poor patient survival. specific inhibition of RAD51 may be an effective means to block DNA repair in MM cells and thus to enhance the efficacy of chemotherapy.

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 339.40 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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6: Takahashi A, Mori E, Nakagawa Y, Kajihara A, Kirita T, Pittman DL, Hasegawa M, Ohnishi T. Homologous recombination preferentially repairs heat-induced DNA double-strand breaks in mammalian cells. Int J Hyperthermia. 2016 Nov 13:1-7. [Epub ahead of print] PubMed PMID: 27776457.

7: David M, Sahay D, Mege F, Descotes F, Leblanc R, Ribeiro J, Clézardin P, Peyruchaud O. Identification of heparin-binding EGF-like growth factor (HB-EGF) as a biomarker for lysophosphatidic acid receptor type 1 (LPA1) activation in human breast and prostate cancers. PLoS One. 2014 May 14;9(5):e97771. doi: 10.1371/journal.pone.0097771. eCollection 2014. PubMed PMID: 24828490; PubMed Central PMCID: PMC4020852.

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12: Kischel P, Bellahcene A, Deux B, Lamour V, Dobson R, DE Pauw E, Clezardin P, Castronovo V. Overexpression of CD9 in human breast cancer cells promotes the development of bone metastases. Anticancer Res. 2012 Dec;32(12):5211-20. PubMed PMID: 23225418.

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14: Huang F, Motlekar NA, Burgwin CM, Napper AD, Diamond SL, Mazin AV. Identification of specific inhibitors of human RAD51 recombinase using high-throughput screening. ACS Chem Biol. 2011 Jun 17;6(6):628-35. doi: 10.1021/cb100428c. Epub 2011 Mar 23. PubMed PMID: 21428443; PubMed Central PMCID: PMC3117970.

15: Fournier PG, Stresing V, Ebetino FH, Clézardin P. How do bisphosphonates inhibit bone metastasis in vivo? Neoplasia. 2010 Jul;12(7):571-8. PubMed PMID: 20651986; PubMed Central PMCID: PMC2907583.

16: Peyruchaud O, Winding B, Pécheur I, Serre CM, Delmas P, Clézardin P. Early detection of bone metastases in a murine model using fluorescent human breast cancer cells: application to the use of the bisphosphonate zoledronic acid in the treatment of osteolytic lesions. J Bone Miner Res. 2001 Nov;16(11):2027-34. PubMed PMID: 11697798.

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18: Contié S, Voorzanger-Rousselot N, Litvin J, Clézardin P, Garnero P. Increased expression and serum levels of the stromal cell-secreted protein periostin in breast cancer bone metastases. Int J Cancer. 2011 Jan 15;128(2):352-60. doi: 10.1002/ijc.25591. Epub 2010 Oct 13. PubMed PMID: 20715172.

19: Morita K, Kimura S, Saito M, Shinoyama H, Usami T, Amemiya Y, Shishido M. Generation and characterization of reduced virulence Fusarium oxysporum f. sp. lycopersici mutants through plasmid-vector insertion. Mycopathologia. 2005 Aug;160(1):67-73. PubMed PMID: 16160771.

20: King HO, Brend T, Payne HL, Wright A, Ward TA, Patel K, Egnuni T, Stead LF, Patel A, Wurdak H, Short SC. RAD51 Is a Selective DNA Repair Target to Radiosensitize Glioma Stem Cells. Stem Cell Reports. 2017 Jan 10;8(1):125-139. doi: 10.1016/j.stemcr.2016.12.005. PubMed PMID: 28076755; PubMed Central PMCID: PMC5233453.