WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H584316
CAS#: 72814-32-5
Description: BW245C has potent platelet anti-aggregating actions following parenteral or oral administration which makes this hydantoin a potentially-useful anti-thrombotic prostaglandin analogue.
Hodoodo Cat#: H584316
Name: BW245C
CAS#: 72814-32-5
Chemical Formula: C19H32N2O5
Exact Mass: 368.23
Molecular Weight: 368.474
Elemental Analysis: C, 61.93; H, 8.75; N, 7.60; O, 21.71
Synonym: BW245C; BW 245C; BW-245C
IUPAC/Chemical Name: 4-Imidazolidineheptanoic acid, 3-((3R)-3-cyclohexyl-3-hydroxypropyl)-2,5-dioxo-, (4S)-rel-
InChi Key: ZIDQIOZJEJFMOH-JKSUJKDBSA-N
InChi Code: InChI=1S/C19H32N2O5/c22-16(14-8-4-3-5-9-14)12-13-21-15(18(25)20-19(21)26)10-6-1-2-7-11-17(23)24/h14-16,22H,1-13H2,(H,23,24)(H,20,25,26)/t15-,16+/m0/s1
SMILES Code: O=C(O)CCCCCC[C@@H](C(N1)=O)N(CC[C@H](C2CCCCC2)O)C1=O
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO
Shelf Life: >2 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info:
| Biological target: | A selective DP1 receptor agonist. |
| In vitro activity: | The specific DP1 agonist BW245c significantly enhanced the percentage of viable cells (Annexin V−/PI−) from 25 to 50% of all eosinophils; by comparison, IL‐5 maintained 59% of the cells viable (Fig. 1A). BW245c concentration‐dependently inhibits apoptosis of eosinophils, with a half maximal efficacy (EC50) of 0.826 μM (Supplementary Fig. 1). PGD2 itself moderately increased the percentage of viable cells to 39%. Reference: J Leukoc Biol. 2018 Jul; 104(1): 159–171. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6032830/ |
| In vivo activity: | Despite being on high fat diet, mice receiving DP1 receptor agonist (BW245C) sustained a significant inhibition of weight gain throughout the study gaining only 11.4% body weight compared to the controls gaining 61% body weight. Reference: Obes Res Clin Pract. 2018 Mar-Apr;12(2):229-241. https://pubmed.ncbi.nlm.nih.gov/28602634/ |
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| PBS (pH 7.2) | 2.4 | 6.43 |
The following data is based on the product molecular weight 368.47 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
| Formulation protocol: | 1. Peinhaupt M, Roula D, Theiler A, Sedej M, Schicho R, Marsche G, Sturm EM, Sabroe I, Rothenberg ME, Heinemann A. DP1 receptor signaling prevents the onset of intrinsic apoptosis in eosinophils and functions as a transcriptional modulator. J Leukoc Biol. 2018 Jul;104(1):159-171. doi: 10.1002/JLB.3MA1017-404R. Epub 2018 Apr 1. PMID: 29607536; PMCID: PMC6032830. 2. Kumar S, Palaia T, Hall C, Ragolia L. DP1 receptor agonist, BW245C inhibits diet-induced obesity in ApoE-/- mice. Obes Res Clin Pract. 2018 Mar-Apr;12(2):229-241. doi: 10.1016/j.orcp.2017.05.003. Epub 2017 Jun 8. PMID: 28602634. 3. van den Brule S, Wallemme L, Uwambayinema F, Huaux F, Lison D. The D prostanoid receptor agonist BW245C [(4S)-(3-[(3R,S)-3-cyclohexyl-3-hydroxypropyl]-2,5-dioxo)-4-imidazolidineheptanoic acid] inhibits fibroblast proliferation and bleomycin-induced lung fibrosis in mice. J Pharmacol Exp Ther. 2010 Nov;335(2):472-9. doi: 10.1124/jpet.110.169250. Epub 2010 Aug 18. PMID: 20719937. |
| In vitro protocol: | 1. Peinhaupt M, Roula D, Theiler A, Sedej M, Schicho R, Marsche G, Sturm EM, Sabroe I, Rothenberg ME, Heinemann A. DP1 receptor signaling prevents the onset of intrinsic apoptosis in eosinophils and functions as a transcriptional modulator. J Leukoc Biol. 2018 Jul;104(1):159-171. doi: 10.1002/JLB.3MA1017-404R. Epub 2018 Apr 1. PMID: 29607536; PMCID: PMC6032830. |
| In vivo protocol: | 1. Kumar S, Palaia T, Hall C, Ragolia L. DP1 receptor agonist, BW245C inhibits diet-induced obesity in ApoE-/- mice. Obes Res Clin Pract. 2018 Mar-Apr;12(2):229-241. doi: 10.1016/j.orcp.2017.05.003. Epub 2017 Jun 8. PMID: 28602634. 2. van den Brule S, Wallemme L, Uwambayinema F, Huaux F, Lison D. The D prostanoid receptor agonist BW245C [(4S)-(3-[(3R,S)-3-cyclohexyl-3-hydroxypropyl]-2,5-dioxo)-4-imidazolidineheptanoic acid] inhibits fibroblast proliferation and bleomycin-induced lung fibrosis in mice. J Pharmacol Exp Ther. 2010 Nov;335(2):472-9. doi: 10.1124/jpet.110.169250. Epub 2010 Aug 18. PMID: 20719937. |
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