WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H562127
CAS#: 2863-04-9
Description: pdTp is a highly selective, small molecule inhibitor of the miRNA regulatory complex RISC subunit SND1.
Hodoodo Cat#: H562127
Name: pdTp
CAS#: 2863-04-9
Chemical Formula: C10H16N2O11P2
Exact Mass: 402.02
Molecular Weight: 402.180
Elemental Analysis: C, 29.86; H, 4.01; N, 6.97; O, 43.76; P, 15.40
This product is not in stock, which may be available by custom synthesis.
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Synonym: pdTp; Thymidine 3',5'-biphosphate;
IUPAC/Chemical Name: [(2R,3S,5R)-5-(5-Methyl-2,4-dioxopyrimidin-1-yl)-2-(phosphonooxymethyl)oxolan-3-yl] dihydrogen phosphate
InChi Key: CSNCBOPUCJOHLS-XLPZGREQSA-N
InChi Code: InChI=1S/C10H16N2O11P2/c1-5-3-12(10(14)11-9(5)13)8-2-6(23-25(18,19)20)7(22-8)4-21-24(15,16)17/h3,6-8H,2,4H2,1H3,(H,11,13,14)(H2,15,16,17)(H2,18,19,20)/t6-,7+,8+/m0/s1
SMILES Code: O=P(O)(O[C@@H]1[C@@H](COP(O)(O)=O)O[C@@H](N(C(N2)=O)C=C(C)C2=O)C1)O
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO
Shelf Life: >2 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info:
| Biological target: | |
| In vitro activity: | |
| In vivo activity: |
The following data is based on the product molecular weight 402.18 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
| Formulation protocol: | |
| In vitro protocol: | |
| In vivo protocol: |
1: Jariwala N, Rajasekaran D, Mendoza RG, Shen XN, Siddiq A, Akiel MA, Robertson CL, Subler MA, Windle JJ, Fisher PB, Sanyal AJ, Sarkar D. Oncogenic Role of SND1 in Development and Progression of Hepatocellular Carcinoma. Cancer Res. 2017 Jun 15;77(12):3306-3316. doi: 10.1158/0008-5472.CAN-17-0298. Epub 2017 Apr 20. PubMed PMID: 28428278; PubMed Central PMCID: PMC5488274.
2: Lopez-Zavala AA, Sotelo-Mundo RR, Hernandez-Flores JM, Lugo-Sanchez ME, Sugich-Miranda R, Garcia-Orozco KD. Arginine kinase shows nucleoside diphosphate kinase-like activity toward deoxythymidine diphosphate. J Bioenerg Biomembr. 2016 Jun;48(3):301-8. doi: 10.1007/s10863-016-9660-1. Epub 2016 Apr 12. PubMed PMID: 27072556.
3: Wang CC, Tsong TY, Hsu YH, Marszalek PE. Inhibitor binding increases the mechanical stability of staphylococcal nuclease. Biophys J. 2011 Feb 16;100(4):1094-9. doi: 10.1016/j.bpj.2011.01.011. Erratum in: Biophys J. 2011 Mar 16;100(6):1596. PubMed PMID: 21320455; PubMed Central PMCID: PMC3037565.
4: Sunil S, Hossain MJ, Ramasamy G, Malhotra P. Transient silencing of Plasmodium falciparum Tudor Staphylococcal Nuclease suggests an essential role for the protein. Biochem Biophys Res Commun. 2008 Jul 25;372(2):373-8. doi: 10.1016/j.bbrc.2008.05.033. Epub 2008 May 16. PubMed PMID: 18486605.
5: Hossain MJ, Korde R, Singh S, Mohmmed A, Dasaradhi PV, Chauhan VS, Malhotra P. Tudor domain proteins in protozoan parasites and characterization of Plasmodium falciparum tudor staphylococcal nuclease. Int J Parasitol. 2008 Apr;38(5):513-26. Epub 2007 Oct 17. PubMed PMID: 18035357.
6: Toure P, Villena F, Melikyan GG. Thymidine 3',5'-diphosphoric acid derived cations and radicals: ab initio study. Org Lett. 2002 Nov 14;4(23):3989-92. PubMed PMID: 12423068.
7: Hirano S, Mihara K, Yamazaki Y, Kamikubo H, Imamoto Y, Kataoka M. Role of C-terminal region of Staphylococcal nuclease for foldability, stability, and activity. Proteins. 2002 Nov 1;49(2):255-65. PubMed PMID: 12211005.
8: Petit CM, Koretke KK. Characterization of Streptococcus pneumoniae thymidylate kinase: steady-state kinetics of the forward reaction and isothermal titration calorimetry. Biochem J. 2002 May 1;363(Pt 3):825-31. PubMed PMID: 11964185; PubMed Central PMCID: PMC1222537.
9: Zhou B, Jing GZ. Conformational features of a truncated staphylococcal nuclease R (SNR135) and their implications for catalysis. Arch Biochem Biophys. 1998 Dec 1;360(1):33-40. PubMed PMID: 9826426.
10: Stein A, Kula MR, Elling L. Combined preparative enzymatic synthesis of dTDP-6-deoxy-4-keto-D-glucose from dTDP and sucrose. Glycoconj J. 1998 Feb;15(2):139-45. PubMed PMID: 9557873.
11: Wang J, Truckses DM, Abildgaard F, Dzakula Z, Zolnai Z, Markley JL. Solution structures of staphylococcal nuclease from multidimensional, multinuclear NMR: nuclease-H124L and its ternary complex with Ca2+ and thymidine-3',5'-bisphosphate. J Biomol NMR. 1997 Sep;10(2):143-64. PubMed PMID: 9369015.
12: Gschwentner M, Jungwirth A, Hofer S, Wöll E, Ritter M, Susanna A, Schmarda A, Reibnegger G, Pinggera GM, Leitinger M, Frick J, Deetjen P, Paulmichl M. Blockade of swelling-induced chloride channels by phenol derivatives. Br J Pharmacol. 1996 May;118(1):41-8. PubMed PMID: 8733574; PubMed Central PMCID: PMC1909500.
13: Loll PJ, Quirk S, Lattman EE, Garavito RM. X-ray crystal structures of staphylococcal nuclease complexed with the competitive inhibitor cobalt(II) and nucleotide. Biochemistry. 1995 Apr 4;34(13):4316-24. PubMed PMID: 7703245.
14: Ulloa RM, Muschietti JP, Veron M, Torres HN, Tellez-Iñón MT. Purification and characterization of a soluble nucleoside diphosphate kinase in Trypanosoma cruzi. Mol Biochem Parasitol. 1995 Mar;70(1-2):119-29. PubMed PMID: 7637692.
15: Creighton TE, Shortle D. Electrophoretic characterization of the denatured states of staphylococcal nuclease. J Mol Biol. 1994 Oct 7;242(5):670-82. PubMed PMID: 7932723.
16: Weber DJ, Libson AM, Gittis AG, Lebowitz MS, Mildvan AS. NMR docking of a substrate into the X-ray structure of the Asp-21-->Glu mutant of staphylococcal nuclease. Biochemistry. 1994 Jul 5;33(26):8017-28. PubMed PMID: 8025106.
17: Libson AM, Gittis AG, Lattman EE. Crystal structures of the binary Ca2+ and pdTp complexes and the ternary complex of the Asp21-->Glu mutant of staphylococcal nuclease. Implications for catalysis and ligand binding. Biochemistry. 1994 Jul 5;33(26):8007-16. PubMed PMID: 8025105.
18: Loh SN, Prehoda KE, Wang J, Markley JL. Hydrogen exchange in unligated and ligated staphylococcal nuclease. Biochemistry. 1993 Oct 19;32(41):11022-8. PubMed PMID: 8218167.
19: Chuang WJ, Weber DJ, Gittis AG, Mildvan AS. Mutational tests of the NMR-docked structure of the staphylococcal nuclease-metal-3',5'-pdTp complex. Proteins. 1993 Sep;17(1):36-48. PubMed PMID: 8234243.
20: Weber DJ, Serpersu EH, Gittis AG, Lattman EE, Mildvan AS. NMR docking of the competitive inhibitor thymidine 3',5'-diphosphate into the X-ray structure of staphylococcal nuclease. Proteins. 1993 Sep;17(1):20-35. PubMed PMID: 8234242.
