PF-06465469
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Hodoodo CAT#: H555203

CAS#: 1407966-77-1

Description: PF-06465469 is a potent, covalent inhibitor of the nonreceptor tyrosine kinase Itk (IL-2 inducible T-cell kinase), which is expressed primarily in immune cells, and is strongly upregulated following activation of T-cells. PF-06465469 inhibits Itk enzymatic activity with an IC50 of 2 nM.

Chemical Structure

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PF-06465469
CAS# 1407966-77-1
Instruction

Theoretical Analysis

Hodoodo Cat#: H555203
Name: PF-06465469
CAS#: 1407966-77-1
Chemical Formula: C30H33N7O2
Exact Mass: 523.27
Molecular Weight: 523.641
Elemental Analysis: C, 68.81; H, 6.35; N, 18.72; O, 6.11

Price and Availability

Size Price Availability Quantity
5mg USD 320 2 Weeks
25mg USD 950 2 Weeks
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Synonym: PF-06465469; PF 06465469; PF06465469; PF-6465469; PF 6465469; PF6465469;

IUPAC/Chemical Name: 3-[4-Amino-1-[(3R)-1-(1-oxo-2-propen-1-yl)-3-piperidinyl]-1H-pyrazolo[3,4-d]pyrimidin-3-yl]-N-[3-methyl-4-(1-methylethyl)phenyl]-benzamide

InChi Key: CGJVMKJGKFEHTL-HSZRJFAPSA-N

InChi Code: InChI=1S/C30H33N7O2/c1-5-25(38)36-13-7-10-23(16-36)37-29-26(28(31)32-17-33-29)27(35-37)20-8-6-9-21(15-20)30(39)34-22-11-12-24(18(2)3)19(4)14-22/h5-6,8-9,11-12,14-15,17-18,23H,1,7,10,13,16H2,2-4H3,(H,34,39)(H2,31,32,33)/t23-/m1/s1

SMILES Code: O=C(NC1=CC=C(C(C)C)C(C)=C1)C2=CC=CC(C3=NN([C@H]4CN(C(C=C)=O)CCC4)C5=NC=NC(N)=C53)=C2

Appearance: Solid powder

Purity: >97% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 523.64 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Zapf CW, Gerstenberger BS, Xing L, Limburg DC, Anderson DR, Caspers N, Han S,
Aulabaugh A, Kurumbail R, Shakya S, Li X, Spaulding V, Czerwinski RM, Seth N,
Medley QG. Covalent inhibitors of interleukin-2 inducible T cell kinase (itk)
with nanomolar potency in a whole-blood assay. J Med Chem. 2012 Nov
26;55(22):10047-63. doi: 10.1021/jm301190s. Epub 2012 Nov 12. PubMed PMID:
23098091.