WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H571614
CAS#: 92921-25-0
Description: Sulfo-MBS Crosslinker, also known as m-Maleimidobenzoyl-N-hydroxysulfosuccinimide ester, is a non-cleavable, water soluble, heterobifunctional protein crosslinking reagent that is commonly used to crosslink haptens to carrier proteins and enzymes to antibody.
Hodoodo Cat#: H571614
Name: Sulfo-MBS Crosslinker
CAS#: 92921-25-0
Chemical Formula: C15H10N2O9S
Exact Mass: 394.01
Molecular Weight: 394.310
Elemental Analysis: C, 45.69; H, 2.56; N, 7.10; O, 36.52; S, 8.13
Synonym: Sulfo-MBS Crosslinker, Sulfo-MBS Cross linker, Sulfo-MBS Cross-linker
IUPAC/Chemical Name: 3-Maleimidobenzoic acid N-hydroxysulfosuccinimide ester
InChi Key: DIYPCWKHSODVAP-UHFFFAOYSA-N
InChi Code: InChI=1S/C15H10N2O9S/c18-11-4-5-12(19)16(11)9-3-1-2-8(6-9)15(22)26-17-13(20)7-10(14(17)21)27(23,24)25/h1-6,10H,7H2,(H,23,24,25)
SMILES Code: O=C(ON1C(C(S(=O)(O)=O)CC1=O)=O)C2=CC=CC(N3C(C=CC3=O)=O)=C2
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO
Shelf Life: >2 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info: The Sulfo-MBS crosslinking reagent's NHS ester and maleimide reactive groups react with amine and sulfhydryl moites, respectively. SATA Protein Modifier can be used to add sulfhydryl groups (thiols, -SH) to proteins or peptides prior to Sulfo-MBS crosslinking.
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The following data is based on the product molecular weight 394.31 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
| Formulation protocol: | |
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1: Chen F, Nielsen S, Zenobi R. Understanding chemical reactivity for homo- and heterobifunctional protein cross-linking agents. J Mass Spectrom. 2013 Jul;48(7):807-12. doi: 10.1002/jms.3224. PubMed PMID: 23832936.
2: Nadeau OW, Carlson GM. Protein Interactions Captured by Chemical Cross-linking: Simple Cross-linking Screen Using Sulfo-MBS. CSH Protoc. 2007 Apr 1;2007:pdb.prot4587. doi: 10.1101/pdb.prot4587. PubMed PMID: 21357052.
3: Kühn-Hölsken E, Lenz C, Dickmanns A, Hsiao HH, Richter FM, Kastner B, Ficner R, Urlaub H. Mapping the binding site of snurportin 1 on native U1 snRNP by cross-linking and mass spectrometry. Nucleic Acids Res. 2010 Sep;38(16):5581-93. doi: 10.1093/nar/gkq272. Epub 2010 Apr 26. PubMed PMID: 20421206; PubMed Central PMCID: PMC2938196.
4: Söllner T, Rassow J, Wiedmann M, Schlossmann J, Keil P, Neupert W, Pfanner N. Mapping of the protein import machinery in the mitochondrial outer membrane by crosslinking of translocation intermediates. Nature. 1992 Jan 2;355(6355):84-7. PubMed PMID: 1530986.
