WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H571622
CAS#: 23024-29-5
Description: DSSeb Crosslinker, or Disuccinimidyl sebacate, is a homobifunctional crosslinking reagent which is cell membrane permeable. DSSeb Crosslinker is useful in protein crosslinking and making antibody drug conjugates (ADCs).
Hodoodo Cat#: H571622
Name: DSSeb Crosslinker
CAS#: 23024-29-5
Chemical Formula: C18H24N2O8
Exact Mass: 396.15
Molecular Weight: 396.396
Elemental Analysis: Molecular Weight: 396.3960 Elemental Analysis: C, 54.54; H, 6.10; N, 7.07; O, 32.29
Synonym: DSSeb Crosslinker, DSSeb Cross linker, DSSeb Cross-linker
IUPAC/Chemical Name: Disuccinimidyl sebacate
InChi Key: XPKIVYVYXKPGBP-UHFFFAOYSA-N
InChi Code: InChI=1S/C18H24N2O8/c21-13-9-10-14(22)19(13)27-17(25)7-5-3-1-2-4-6-8-18(26)28-20-15(23)11-12-16(20)24/h1-12H2
SMILES Code: O=C(ON1C(CCC1=O)=O)CCCCCCCCC(ON2C(CCC2=O)=O)=O
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO
Shelf Life: >2 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info: DSSeb crosslinking takes place via amine-reactive NHS esters at both ends of a 10-atom (14.1 Angstrom) spacer arm. DSSeb must be dissolved in an organic solvent, such as DMSO or DMF, then added to an aqueous crosslinking reaction.
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The following data is based on the product molecular weight 396.40 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
| Formulation protocol: | |
| In vitro protocol: | |
| In vivo protocol: |
1: Heck T, Faccio G, Richter M, Thöny-Meyer L. Enzyme-catalyzed protein crosslinking. Appl Microbiol Biotechnol. 2013 Jan;97(2):461-75. doi: 10.1007/s00253-012-4569-z. Epub 2012 Nov 25. Review. PubMed PMID: 23179622; PubMed Central PMCID: PMC3546294.
2: Reddy N, Reddy R, Jiang Q. Crosslinking biopolymers for biomedical applications. Trends Biotechnol. 2015 Jun;33(6):362-9. doi: 10.1016/j.tibtech.2015.03.008. Epub 2015 Apr 14. Review. PubMed PMID: 25887334.
3: Movahedi A, Lundin A, Kann N, Nydén M, Moth-Poulsen K. Cu(I) stabilizing crosslinked polyethyleneimine. Phys Chem Chem Phys. 2015 Jul 28;17(28):18327-36. doi: 10.1039/c5cp02198g. PubMed PMID: 26104912.
4: Reinitz SD, Currier BH, Levine RA, Van Citters DW. Crosslink density, oxidation and chain scission in retrieved, highly cross-linked UHMWPE tibial bearings. Biomaterials. 2014 May;35(15):4436-40. doi: 10.1016/j.biomaterials.2014.02.019. Epub 2014 Mar 1. PubMed PMID: 24589360.
5: Javadi MA, Feizi S. Sterile Keratitis following Collagen Crosslinking. J Ophthalmic Vis Res. 2014 Oct-Dec;9(4):510-3. doi: 10.4103/2008-322X.150832. PubMed PMID: 25709779; PubMed Central PMCID: PMC4329714.
