1M7
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    WARNING: This product is for research use only, not for human or veterinary use.

Hodoodo CAT#: H584583

CAS#: 73043-80-8

Description: 1M7 is a reagent for RNA SHAPE-MaP experiments that enables analysis of RNA secondary structures at single nucleotide resolution in <70 seconds.

Chemical Structure

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1M7
CAS# 73043-80-8
Instruction

Theoretical Analysis

Hodoodo Cat#: H584583
Name: 1M7
CAS#: 73043-80-8
Chemical Formula: C9H6N2O5
Exact Mass: 222.03
Molecular Weight: 222.156
Elemental Analysis: C, 48.66; H, 2.72; N, 12.61; O, 36.01

Price and Availability

Size Price Availability Quantity
10mg USD 400 2 Weeks
1g USD 3250 2 Weeks
2g USD 5450 2 Weeks
5g USD 9850 2 Weeks
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Synonym: 1M7

IUPAC/Chemical Name: 1-Methyl-7-nitro-2H-3,1-benzoxazine-2,4(1H)-dione

InChi Key: MULNCJWAVSDEKJ-UHFFFAOYSA-N

InChi Code: InChI=1S/C9H6N2O5/c1-10-7-4-5(11(14)15)2-3-6(7)8(12)16-9(10)13/h2-4H,1H3

SMILES Code: O=C(O1)N(C)C2=CC([N+]([O-])=O)=CC=C2C1=O

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 222.16 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

Molarity Calculator

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*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / CoA (available online).

Reconstitution Calculator

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Dilution Calculator

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Mortimer et al (2007) A fast-acting reagent for accurate analysis of RNA secondary and tertiary structure by SHAPE chemistry. J.Am.Chem.Soc. 129 4144 PMID: 17367143

Turner et al (2013) Safer one-pot synthesis of the 'SHAPE' reagent 1-methyl-7-nitroisatoic anhydride (1m7). RNA 19 1857 PMID: 24141619

Siegfried et al (2014) RNA motif discovery by SHAPE and mutational profiling (SHAPE-MaP). Nat.Methods 11 959 PMID: 25028896

Smola et al (2015) Detection of RNA-Protein interactions in living cells with SHAPE. Biochemistry 54 6867 PMID: 26544910