WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H574003
CAS#: 1325307-57-0
Description: Bz-IEGR-pNA acetate is a colorimetric substrate for Factor Xa. Factor Xa preferentially binds to and cleaves the Ile-Glu-Gly-Arg (IEGR) peptide sequence to release p-nitroanilide (pNA), which can be quantified by colorimetric detection at 405 nm as a measure of Factor Xa activity.
Hodoodo Cat#: H574003
Name: Bz-IEGR-pNA acetate
CAS#: 1325307-57-0
Chemical Formula: C34H47N9O11
Exact Mass: 757.34
Molecular Weight: 757.800
Elemental Analysis: C, 53.89; H, 6.25; N, 16.64; O, 23.22
Synonym: Bz-IEGR-pNA acetate, Bz-Ile-Glu-Gly-Arg-pNA, Bz-Ile-Glu-Gly-Arg-p-nitroanilide, Factor Xa Chromogenic Substrate
IUPAC/Chemical Name: N-benzoyl-L-isoleucyl-L-α-glutamylglycyl-N-(4-nitrophenyl)-L-argininamide, monoacetate
InChi Key: ITXQNZUGLZNGBM-PJSBSAQXSA-N
InChi Code: InChI=1S/C32H43N9O9.C2H4O2/c1-3-19(2)27(40-28(45)20-8-5-4-6-9-20)31(48)39-24(15-16-26(43)44)29(46)36-18-25(42)38-23(10-7-17-35-32(33)34)30(47)37-21-11-13-22(14-12-21)41(49)50;1-2(3)4/h4-6,8-9,11-14,19,23-24,27H,3,7,10,15-18H2,1-2H3,(H,36,46)(H,37,47)(H,38,42)(H,39,48)(H,40,45)(H,43,44)(H4,33,34,35);1H3,(H,3,4)/t19-,23-,24-,27-;/m0./s1
SMILES Code: O=C([C@H](CCCNC(N)=N)NC(CNC([C@@H](NC([C@](NC(C1=CC=CC=C1)=O)([H])[C@@H](C)CC)=O)CCC(O)=O)=O)=O)NC2=CC=C([N+]([O-])=O)C=C2.CC(O)=O
Appearance: A crystalline solid
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO
Shelf Life: >3 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info: λmax: 224, 314 nm
| Biological target: | |
| In vitro activity: | |
| In vivo activity: |
The following data is based on the product molecular weight 757.80 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
| Formulation protocol: | |
| In vitro protocol: | |
| In vivo protocol: |
1. Aruell, L., Friberger, P., Karlsson, G., et al. A new sensitive and highly specific chromogenic peptide substrate for factor Xa. Thromb. Res. 11(5), 595-609 (1977).
