WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H574175
CAS#: 142234-85-3
Description: N-methyl Mesoporphyrin IX is a transition state analog of porphyrin and an inhibitor of ferrochelatase. N-methyl Mesoporphyrin IX has been used as a turn-on biosensor for target DNA sequences when used in complex with a G-quadruplex-forming sequence fused to a DNA sequence complementary to the target sequence. It has also been used to detect amyloid-β (1-40) (Aβ40) fibrils in vitro and in live PC12 cells overexpressing Aβ.
Hodoodo Cat#: H574175
Name: N-methyl Mesoporphyrin IX
CAS#: 142234-85-3
Chemical Formula: C35H40N4O4
Exact Mass: 580.31
Molecular Weight: 580.730
Elemental Analysis: C, 72.39; H, 6.94; N, 9.65; O, 11.02
Synonym: N-methyl Mesoporphyrin IX
IUPAC/Chemical Name: 8,13-diethyl-3,7,12,17,23-pentamethyl-21H,23H-porphine-2,18-dipropanoic acid
InChi Key: XASXJWXLPCEQJN-UQOLDQHFSA-N
InChi Code: InChI=1S/C35H40N4O4/c1-8-22-18(3)26-14-27-19(4)24(10-12-34(40)41)29(36-27)15-30-25(11-13-35(42)43)20(5)28(38-30)16-33-23(9-2)21(6)32(39(33)7)17-31(22)37-26/h14-17,36H,8-13H2,1-7H3,(H,40,41)(H,42,43)/b26-14-,27-14-,28-16-,29-15-,30-15-,31-17-,32-17-,33-16-
SMILES Code: CC1=C2[NH]C(/C=C(C(CCC(O)=O)=C3C)\[N]=C3/C=C4[N](C)/C(C(C)=C\4CC)=C\C5=[N]/C(C(C)=C5CC)=C\2)=C1CCC(O)=O
Appearance: Solid powder
Purity: >95% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO
Shelf Life: >3 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info: N-methyl Mesoporphyrin displays excitation/emission maxima of 399/610 nm, respectively.
Biological target: | |
In vitro activity: | |
In vivo activity: |
The following data is based on the product molecular weight 580.73 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
---|---|---|---|
1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
Formulation protocol: | |
In vitro protocol: | |
In vivo protocol: |
1. de Matteis, F., Gibbs, A.H., and Smith, A.G. Inhibition of protohaem ferro-lyase by N-substituted porphyrins. Structural requirements for the inhibitory effect. Biochem. J. 189(3), 645-648 (1980).
2. Ren, J., Qin, H., Wang, J., et al. Label-free detection of nucleic acids by turn-on and turn-off G-quadruplex-mediated fluorescence. Anal. Bioanal. Chem. 399(8), 2763-2770 (2011).
3. Li, M., Zhao, A., Ren, J., et al. N‑Methyl mesoporphyrin IX as an effective probe for monitoring Alzheimer’s disease β‑amyloid aggregation in living cells. ACS Chem. Neurosci. 8(6), 1299-1304 (2017).
4. Paramasivan, S., and Bolton, P.H. Mix and measure fluorescence screening for selective quadruplex binders. Nucleic Acids Res. 36(17), e106 (2008).