PF-04449613
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Hodoodo CAT#: H462312

CAS#: 1236858-52-8

Description: PF-04449613 is a phosphodiesterase 9A (PDE9A) inhibitor. Subcutaneous administration of PF-04449613 (10 mg/kg) increases the rate of dendritic spine formation and elimination in mouse primary motor cortex pyramidal neurons in vivo.

Chemical Structure

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PF-04449613
CAS# 1236858-52-8
Instruction

Theoretical Analysis

Hodoodo Cat#: H462312
Name: PF-04449613
CAS#: 1236858-52-8
Chemical Formula: C21H25N5O3
Exact Mass: 395.20
Molecular Weight: 395.463
Elemental Analysis: C, 63.78; H, 6.37; N, 17.71; O, 12.14

Price and Availability

Size Price Availability Quantity
5mg USD 280 2 Weeks
10mg USD 850 2 Weeks
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Synonym: PF-04449613; PF 04449613; PF04449613

IUPAC/Chemical Name: 1,5-dihydro-6-[(1R)-1-(3-phenoxy-1-azetidinyl)ethyl]-1-(tetrahydro-2H-pyran-4-yl)-4H-pyrazolo[3,4-d]pyrimidin-4-one

InChi Key: FHBANDDJQJAZOQ-CQSZACIVSA-N

InChi Code: InChI=1S/C21H25N5O3/c1-14(25-12-17(13-25)29-16-5-3-2-4-6-16)19-23-20-18(21(27)24-19)11-22-26(20)15-7-9-28-10-8-15/h2-6,11,14-15,17H,7-10,12-13H2,1H3,(H,23,24,27)/t14-/m1/s1

SMILES Code: O=C1N=C([C@@H](C)N2CC(OC3=CC=CC=C3)C2)NC4=C1C=NN4C5CCOCC5

Appearance: Solid powder

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >3 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 395.46 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1. Claffey, M.M., Helal, C.J., Verhoest, P.R., et al. Application of structure-based drug design and parallel chemistry to identify selective, brain penetrant, in vivo active phosphodiesterase 9A inhibitors. J. Med. Chem. 55(21), 9055-9068 (2012).

2. Lai, B., Li, M., Hu, A., et al. The phosphodiesterase 9 inhibitor PF-04449613 promotes dendritic spine formation and performance improvement after motor learning. Dev. Neurobiol. 78(9), 859-872 (2018).