WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H463499
CAS#: 1621326-32-6
Description: para-NitroBlebbistatin is a selective cell-permeable inhibitor of non-muscle myosin II ATPases, being more stable and less phototoxic than Blebbistatin.
Hodoodo Cat#: H463499
Name: para-NitroBlebbistatin
CAS#: 1621326-32-6
Chemical Formula: C18H15N3O4
Exact Mass: 337.11
Molecular Weight: 337.335
Elemental Analysis: C, 64.09; H, 4.48; N, 12.46; O, 18.97
Synonym: para-NitroBlebbistatin; para NitroBlebbistatin; para-Nitro Blebbistatin; para-Nitro-Blebbistatin; (–)-4'-amino-Blebbistatin; p-amino-Blebbistatin; (S)-4'-amino-Blebbistatin;
IUPAC/Chemical Name: (S)-3a-hydroxy-6-methyl-1-(4-nitrophenyl)-1,2,3,3a-tetrahydro-4H-pyrrolo[2,3-b]quinolin-4-one
InChi Key: KAUXNLHXGQGFOS-GOSISDBHSA-N
InChi Code: InChI=1S/C18H15N3O4/c1-11-2-7-15-14(10-11)16(22)18(23)8-9-20(17(18)19-15)12-3-5-13(6-4-12)21(24)25/h2-7,10,23H,8-9H2,1H3/t18-/m1/s1
SMILES Code: O=C1[C@@]2(O)C(N(C3=CC=C([N+]([O-])=O)C=C3)CC2)=NC4=C1C=C(C)C=C4
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO
Shelf Life: >3 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info:
Biological target: | |
In vitro activity: | |
In vivo activity: |
The following data is based on the product molecular weight 337.34 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
---|---|---|---|
1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
Formulation protocol: | |
In vitro protocol: | |
In vivo protocol: |
1: Tang W, Blair CA, Walton SD, Málnási-Csizmadia A, Campbell KS, Yengo CM. Modulating Beta-Cardiac Myosin Function at the Molecular and Tissue Levels. Front Physiol. 2017 Jan 9;7:659. doi: 10.3389/fphys.2016.00659. PMID: 28119616; PMCID: PMC5220080.
2: Várkuti BH, Képiró M, Horváth IÁ, Végner L, Ráti S, Zsigmond Á, Hegyi G, Lenkei Z, Varga M, Málnási-Csizmadia A. A highly soluble, non-phototoxic, non- fluorescent blebbistatin derivative. Sci Rep. 2016 May 31;6:26141. doi: 10.1038/srep26141. PMID: 27241904; PMCID: PMC4886532.
3: Képiró M, Várkuti BH, Végner L, Vörös G, Hegyi G, Varga M, Málnási-Csizmadia A. para-Nitroblebbistatin, the non-cytotoxic and photostable myosin II inhibitor. Angew Chem Int Ed Engl. 2014 Jul 28;53(31):8211-5. doi: 10.1002/anie.201403540. Epub 2014 Jun 20. PMID: 24954740.