WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H406600
CAS#: 1533426-72-0
Description: SCR7 is a specific DNA Ligase IV inhibitor. SCR7 inhibits end joining of double strand breaks in diverse cell types resulting in tumour regression by activation of p53 mediated apoptosis. Notably SCR7 treatment did not result in any adverse effects in mice and did not inhibit Ligase III. The therapeutic efficacy of SCR7 could be enhanced by specific delivery of SCR7 to the tumour tissue and as adjuvant cancer therapy. SCR7 appears to be a potential cytotoxic anti-cancer drug candidate that can be used either alone or in combination with conventional DNA damaging drugs, owing to its specificity and absence of adverse effects in mice model.
Hodoodo Cat#: H406600
Name: SCR7
CAS#: 1533426-72-0
Chemical Formula: C18H14N4OS
Exact Mass: 334.09
Molecular Weight: 334.390
Elemental Analysis: C, 64.65; H, 4.22; N, 16.75; O, 4.78; S, 9.59
Synonym: SCR7; SCR-7; SCR 7.
IUPAC/Chemical Name: 5,6-bis((E)-benzylideneamino)-2-thioxo-2,3-dihydropyrimidin-4(1H)-one
InChi Key: NEEVCWPRIZJJRJ-AYKLPDECSA-N
InChi Code: InChI=1S/C18H14N4OS/c23-17-15(19-11-13-7-3-1-4-8-13)16(21-18(24)22-17)20-12-14-9-5-2-6-10-14/h1-12H,(H2,21,22,23,24)/b19-11+,20-12+
SMILES Code: O=C(C(/N=C/C1=CC=CC=C1)=C(/N=C/C2=CC=CC=C2)N3)NC3=S
Appearance: Yellow solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO, not in water
Shelf Life: >2 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info: In solution, SCR7 may exist in two tauomers (tautomer A and tautomer B below). Both tauomers are identical. NMR analysis in DMSO showed that tautomer A is the predominant one. Please see our NMR test result in QC data: current batch, Lot#SSC50215, in which there are two NH groups (chemical shifts at 13.4ppm and 12.8ppm). Tautomers are constitutional isomers of organic compounds that readily interconvert by a chemical reaction called tautomerization. This reaction commonly results in the formal migration of a hydrogen atom or proton, accompanied by a switch of a single bond and adjacent double bond. The concept of tautomerizations is called tautomerism. Because of the rapid interconversion, tautomers are generally considered to be the same chemical compound. (copies from http://en.wikipedia.org/wiki/Tautomer). Tautomers are constitutional isomers of organic compounds that readily interconvert by a chemical reaction called tautomerization. This reaction commonly results in the formal migration of a hydrogen atom or proton, accompanied by a switch of a single bond and adjacent double bond. The concept of tautomerizations is called tautomerism. Because of the rapid interconversion, tautomers are generally considered to be the same chemical compound. (copies from http://en.wikipedia.org/wiki/Tautomer).
| Biological target: | SCR7 is a specific DNA Ligase IV inhibitor, which blocks nonhomologous end-joining (NHEJ). |
| In vitro activity: | NHEJ inhibitors could be used in conjunction with standard therapy (etoposide and cisplatin) for colon cancer treatments. In a LoVo human colorectal adenocarcinoma cell line, the combination of SCR7 and NU7441 synergistically increased the cytotoxicity of cisplatin and etoposide, but the effect of SCR7 was more pronounced. SCR7 and NU7441 also significantly increased etoposide. Reference: Mol Biol Rep. 2021 Jan;48(1):709-720. https://pubmed.ncbi.nlm.nih.gov/33389482/ |
| In vivo activity: | SCR7 was found to be effective in increasing oligonucleotide-based knock-in improved genome-editing via oviductal nucleic acids (i-GONAD) delivery in rats. These findings will help advance in situ genome editing. Reference: BMC Biotechnol. 2021 Nov 1;21(1):63. https://pubmed.ncbi.nlm.nih.gov/34724929/ |
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 66.0 | 198.57 | |
| Ethanol | 3.0 | 9.03 |
The following data is based on the product molecular weight 334.39 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
| Formulation protocol: | 1. Anuchina AA, Zaynitdinova MI, Demchenko AG, Evtushenko NA, Lavrov AV, Smirnikhina SA. Bridging Gaps in HDR Improvement: The Role of MAD2L2, SCAI, and SCR7. Int J Mol Sci. 2023 Apr 4;24(7):6704. doi: 10.3390/ijms24076704. PMID: 37047677; PMCID: PMC10095018. 2. Kopa P, Macieja A, Pastwa E, Majsterek I, Poplawski T. DNA double-strand breaks repair inhibitors potentiates the combined effect of VP-16 and CDDP in human colorectal adenocarcinoma (LoVo) cells. Mol Biol Rep. 2021 Jan;48(1):709-720. doi: 10.1007/s11033-020-06124-9. Epub 2021 Jan 2. PMID: 33389482. 3. Aoshima T, Kobayashi Y, Takagi H, Iijima K, Sato M, Takabayashi S. Modification of improved-genome editing via oviductal nucleic acids delivery (i-GONAD)-mediated knock-in in rats. BMC Biotechnol. 2021 Nov 1;21(1):63. doi: 10.1186/s12896-021-00723-5. PMID: 34724929; PMCID: PMC8561937. 4. Gopalakrishnan V, Sharma S, Ray U, Manjunath M, Lakshmanan D, Vartak SV, Gopinatha VK, Srivastava M, Kempegowda M, Choudhary B, Raghavan SC. SCR7, an inhibitor of NHEJ can sensitize tumor cells to ionization radiation. Mol Carcinog. 2021 Sep;60(9):627-643. doi: 10.1002/mc.23329. Epub 2021 Jun 30. PMID: 34192388. |
| In vitro protocol: | 1. Anuchina AA, Zaynitdinova MI, Demchenko AG, Evtushenko NA, Lavrov AV, Smirnikhina SA. Bridging Gaps in HDR Improvement: The Role of MAD2L2, SCAI, and SCR7. Int J Mol Sci. 2023 Apr 4;24(7):6704. doi: 10.3390/ijms24076704. PMID: 37047677; PMCID: PMC10095018. 2. Kopa P, Macieja A, Pastwa E, Majsterek I, Poplawski T. DNA double-strand breaks repair inhibitors potentiates the combined effect of VP-16 and CDDP in human colorectal adenocarcinoma (LoVo) cells. Mol Biol Rep. 2021 Jan;48(1):709-720. doi: 10.1007/s11033-020-06124-9. Epub 2021 Jan 2. PMID: 33389482. |
| In vivo protocol: | 1. Aoshima T, Kobayashi Y, Takagi H, Iijima K, Sato M, Takabayashi S. Modification of improved-genome editing via oviductal nucleic acids delivery (i-GONAD)-mediated knock-in in rats. BMC Biotechnol. 2021 Nov 1;21(1):63. doi: 10.1186/s12896-021-00723-5. PMID: 34724929; PMCID: PMC8561937. 2. Gopalakrishnan V, Sharma S, Ray U, Manjunath M, Lakshmanan D, Vartak SV, Gopinatha VK, Srivastava M, Kempegowda M, Choudhary B, Raghavan SC. SCR7, an inhibitor of NHEJ can sensitize tumor cells to ionization radiation. Mol Carcinog. 2021 Sep;60(9):627-643. doi: 10.1002/mc.23329. Epub 2021 Jun 30. PMID: 34192388. |
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2: John F, George J, Vartak SV, Srivastava M, Hassan PA, Aswal VK, Karki SS, Raghavan SC. Enhanced Efficacy of Pluronic Copolymer Micelle Encapsulated SCR7 against Cancer Cell Proliferation. Macromol Biosci. 2014 Dec 16. doi: 10.1002/mabi.201400480. [Epub ahead of print] PubMed PMID: 25515310.
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