WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H464428
CAS#: 10010-67-0
Description: PIPES is frequently used as a buffering agent in biochemistry. It is useful in cell culture work. It has been documented to minimize lipid loss when buffering glutaraldehyde histology in plant and animal tissues.
Hodoodo Cat#: H464428
Name: PIPES monosodium
CAS#: 10010-67-0
Chemical Formula: C8H17N2NaO6S2
Exact Mass: 0.00
Molecular Weight: 324.342
Elemental Analysis: C, 29.63; H, 5.28; N, 8.64; Na, 7.09; O, 29.60; S, 19.77
Synonym: PIPES monosodium;
IUPAC/Chemical Name: sodium 2-(4-(2-sulfoethyl)piperazin-1-yl)ethane-1-sulfonate
InChi Key: OGGAIRCLBMGXCZ-UHFFFAOYSA-M
InChi Code: InChI=1S/C8H18N2O6S2.Na/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16;/h1-8H2,(H,11,12,13)(H,14,15,16);/q;+1/p-1
SMILES Code: OS(=O)(CCN1CCN(CC1)CCS(=O)([O-])=O)=O.[Na+]
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: To be determined
Shelf Life: >2 years if stored properly
Drug Formulation: To be determined
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info:
| Biological target: | |
| In vitro activity: | |
| In vivo activity: |
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| 0.1M NaOH | 250.0 | 0.00 |
The following data is based on the product molecular weight 324.34 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
| Formulation protocol: | |
| In vitro protocol: | |
| In vivo protocol: |
1: Arakawa T, Timasheff SN. The mechanism of action of Na glutamate, lysine HCl, and piperazine-N,N'-bis(2-ethanesulfonic acid) in the stabilization of tubulin and microtubule formation. J Biol Chem. 1984 Apr 25;259(8):4979-86. PMID: 6325414.
2: Roy RN, Gibbons JJ, Padron JL, Buechter K, Faszholz S, Doan H. Revised values of the paH of monosodium 1,4-piperazinediethanesulfonate ("Pipes') in water and other buffers in isotonic saline at various temperatures. Clin Chem. 1981 Oct;27(10):1787-8. PMID: 6269781.
