WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H464673
CAS#: 70363-83-6
Description: DiBAC4(3) is a negatively charged fluorescent membrane potential indicator. Upon membrane depolarization, DiBAC4(3) enters the cytosol, binds to lipid membranes and intracellular proteins, and displays excitation/emission maxima of 490/516 nm, respectively. It has been used to measure the membrane potential of live or fixed mammalian and bacterial cells by flow cytometry or fluorescence microscopy.
Hodoodo Cat#: H464673
Name: DiBAC4(3)
CAS#: 70363-83-6
Chemical Formula: C27H40N4O6
Exact Mass: 516.29
Molecular Weight: 516.639
Elemental Analysis: C, 62.77; H, 7.80; N, 10.84; O, 18.58
Synonym: DiBAC4(3); DiBAC4 (3); DiBAC4-(3);
IUPAC/Chemical Name: (E)-1,3-dibutyl-5-(3-(1,3-dibutyl-2,4,6-trioxohexahydropyrimidin-5-yl)allylidene)pyrimidine-2,4,6(1H,3H,5H)-trione
InChi Key: ATJCYSYHWGQAET-BUHFOSPRSA-N
InChi Code: InChI=1S/C27H40N4O6/c1-5-9-16-28-22(32)20(23(33)29(26(28)36)17-10-6-2)14-13-15-21-24(34)30(18-11-7-3)27(37)31(25(21)35)19-12-8-4/h13-15,20H,5-12,16-19H2,1-4H3/b14-13+
SMILES Code: O=C1/C(C(N(C(N1CCCC)=O)CCCC)=O)=C\C=C\C2C(N(C(N(C2=O)CCCC)=O)CCCC)=O
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: To be determined
Shelf Life: >2 years if stored properly
Drug Formulation: To be determined
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info:
Biological target: | |
In vitro activity: | |
In vivo activity: |
Solvent | Max Conc. mg/mL | Max Conc. mM | |
---|---|---|---|
Solubility | |||
DMF | 10.0 | 19.36 | |
DMSO | 15.0 | 29.04 | |
DMSO:PBS (pH 7.2) (1:2) | 0.3 | 0.64 | |
Ethanol | 5.0 | 9.68 |
The following data is based on the product molecular weight 516.64 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
---|---|---|---|
1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
Formulation protocol: | |
In vitro protocol: | |
In vivo protocol: |
1: Kim H, Lee DG. Lupeol-induced nitric oxide elicits apoptosis-like death within Escherichia coli in a DNA fragmentation-independent manner. Biochem J. 2021 Feb 26;478(4):855-869. doi: 10.1042/BCJ20200925. PMID: 33522568.
2: Nordström T, Zhenyu G, Andersson LC, Åkerman KEO. Tannic acid inhibits electrogenic Na+/HCO3- co-transporter activity in embryonic neural stem cell- derived radial glial-like cells. Neuroreport. 2020 Jan 8;31(1):57-63. doi: 10.1097/WNR.0000000000001372. PMID: 31714480; PMCID: PMC6903378.
3: Martelli A, Citi V, Calderone V. Vascular Effects of H2S-Donors: Fluorimetric Detection of H2S Generation and Ion Channel Activation in Human Aortic Smooth Muscle Cells. Methods Mol Biol. 2019;2007:79-87. doi: 10.1007/978-1-4939-9528-8_6. PMID: 31148107.
4: Wang Q, Xu Y, Dong M, Hang B, Sun Y, Wang L, Wang Y, Hu J, Zhang W. HJH-1, a Broad-Spectrum Antimicrobial Activity and Low Cytotoxicity Antimicrobial Peptide. Molecules. 2018 Aug 14;23(8):2026. doi: 10.3390/molecules23082026. PMID: 30110916; PMCID: PMC6222697.
5: Zhang P, Turnbull MW. Virus innexin expression in insect cells disrupts cell membrane potential and pH. J Gen Virol. 2018 Oct;99(10):1444-1452. doi: 10.1099/jgv.0.001132. Epub 2018 Aug 9. PMID: 30091698.
6: Murray JW, Yin D, Wolkoff AW. Reduction of organelle motility by removal of potassium and other solutes. PLoS One. 2017 Sep 18;12(9):e0184898. doi: 10.1371/journal.pone.0184898. PMID: 28922372; PMCID: PMC5602639.
7: Yun DG, Lee DG. Assessment of silibinin as a potential antifungal agent and investigation of its mechanism of action. IUBMB Life. 2017 Aug;69(8):631-637. doi: 10.1002/iub.1647. Epub 2017 Jun 21. PMID: 28636236.
8: Lin TS, Tsai HJ, Lee CH, Song YQ, Huang RS, Hsieh-Li HM, Liang MC, Lin Y. An Improved Drugs Screening System Reveals that Baicalein Ameliorates the Aβ/AMPA/NMDA-Induced Depolarization of Neurons. J Alzheimers Dis. 2017;56(3):959-976. doi: 10.3233/JAD-160898. PMID: 28106556.
9: Guo Y, Stärk HJ, Hause G, Schmidt M, Harms H, Wick LY, Müller S. Heterogenic response of prokaryotes toward silver nanoparticles and ions is facilitated by phenotypes and attachment of silver aggregates to cell surfaces. Cytometry A. 2017 Aug;91(8):775-784. doi: 10.1002/cyto.a.23055. Epub 2017 Jan 22. PMID: 28110496.
10: Pina-Vaz C, Silva AP, Faria-Ramos I, Teixeira-Santos R, Moura D, Vieira TF, Sousa SF, Costa-de-Oliveira S, Cantón R, Rodrigues AG. A Flow Cytometric and Computational Approaches to Carbapenems Affinity to the Different Types of Carbapenemases. Front Microbiol. 2016 Aug 9;7:1259. doi: 10.3389/fmicb.2016.01259. PMID: 27555844; PMCID: PMC4977277.