WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H466247
CAS#: 1015698-39-1
Description: Ac4ManNDAz is a cell-permeable photo-crosslinking probe for studying glycoprotein or glycolipid interactions. The compound is an unnatural diazirine-containing monosaccharide building block, which acts as a photo-crosslinker. Once inside the cell, Ac4ManNDAz is metabolized and incorporated as photo-crosslinking sialic acid analogs into cell surface glycoproteins. Upon photoactivation with UV irradiation, the sialic acid appended diazirine is activated to a reactive carbene that covalently crosslinks to nearby molecules.
Hodoodo Cat#: H466247
Name: Ac4ManNDAz
CAS#: 1015698-39-1
Chemical Formula: C19H27N3O10
Exact Mass: 457.17
Molecular Weight: 457.436
Elemental Analysis: C, 49.89; H, 5.95; N, 9.19; O, 34.98
Synonym: Ac4ManNDAz; Ac4-ManNDAz; Ac4 ManNDAz;
IUPAC/Chemical Name: (3S,4R,5S,6R)-6-(acetoxymethyl)-3-(3-(3-methyl-3H-diazirin-3-yl)propanamido)tetrahydro-2H-pyran-2,4,5-triyl triacetate
InChi Key: UZYLHDJBFVVKBM-GAMQSXCYSA-N
InChi Code: InChI=1S/C19H27N3O10/c1-9(23)28-8-13-16(29-10(2)24)17(30-11(3)25)15(18(32-13)31-12(4)26)20-14(27)6-7-19(5)21-22-19/h13,15-18H,6-8H2,1-5H3,(H,20,27)/t13-,15+,16-,17-,18?/m1/s1
SMILES Code: CC(OC[C@H]1OC(OC(C)=O)[C@@H](NC(CCC2(C)N=N2)=O)[C@@H](OC(C)=O)[C@@H]1OC(C)=O)=O
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: To be determined
Shelf Life: >2 years if stored properly
Drug Formulation: To be determined
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info:
Biological target: | |
In vitro activity: | |
In vivo activity: |
Solvent | Max Conc. mg/mL | Max Conc. mM | |
---|---|---|---|
Solubility | |||
Ethanol | 45.7 | 100.00 | |
DMSO | 45.7 | 100.00 |
The following data is based on the product molecular weight 457.44 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
---|---|---|---|
1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
Formulation protocol: | |
In vitro protocol: | |
In vivo protocol: |
1: Pham ND, Fermaintt CS, Rodriguez AC, McCombs JE, Nischan N, Kohler JJ. Cellular metabolism of unnatural sialic acid precursors. Glycoconj J. 2015 Oct;32(7):515-29. doi: 10.1007/s10719-015-9593-7. Epub 2015 May 10. PMID: 25957566; PMCID: PMC4641449.