Pentoxyresorufin
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    WARNING: This product is for research use only, not for human or veterinary use.

Hodoodo CAT#: H112159

CAS#: 87687-03-4

Description: Pentoxyresorufin is a fluorogenic substrate for cytochrome P450 (CYP) enzymes.

Chemical Structure

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Pentoxyresorufin
CAS# 87687-03-4
Instruction

Theoretical Analysis

Hodoodo Cat#: H112159
Name: Pentoxyresorufin
CAS#: 87687-03-4
Chemical Formula: C17H17NO3
Exact Mass: 283.12
Molecular Weight: 283.330
Elemental Analysis: C, 72.07; H, 6.05; N, 4.94; O, 16.94

Price and Availability

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1mg USD 280 2 Weeks
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Synonym: Pentoxyresorufin; 7-Pentoxyresorufin; O-Pentylresorufin

IUPAC/Chemical Name: 7-(pentyloxy)-3H-phenoxazin-3-one

InChi Key: ZPSOKQFFOYYPKC-UHFFFAOYSA-N

InChi Code: InChI=1S/C17H17NO3/c1-2-3-4-9-20-13-6-8-15-17(11-13)21-16-10-12(19)5-7-14(16)18-15/h5-8,10-11H,2-4,9H2,1H3

SMILES Code: O=C1C=CC2=NC3=C(OC2=C1)C=C(C=C3)OCCCCC

Appearance: Solid powder

Purity: >95% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: To be determined

Shelf Life: >2 years if stored properly

Drug Formulation: To be determined

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 283.33 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Zamaratskaia G, Zlabek V, Ropstad E, Tajet H, Andresen Ø. Hepatic ethoxy-, methoxy- and pentoxyresorufin O-dealkylase activities in Landrace and Duroc pigs stimulated with HCG. Reprod Domest Anim. 2010 Dec;45(6):e269-74. doi: 10.1111/j.1439-0531.2009.01553.x. PMID: 19930133.


2: Alterman MA, Carvan MJ, Busbee DL. Ethoxyresorufin and pentoxyresorufin O-dealkylation by hepatic microsomes from female Fischer 344 rats: effects of age and diet. Mech Ageing Dev. 1994 Nov 25;77(1):1-11. doi: 10.1016/0047-6374(94)90042-6. PMID: 7731269.


3: Veres Z, Szakács T, Vereczkey L. Effect of alpha-methyldopa on pentoxyresorufin O-dealkylation in liver microsomes from rats treated with phenobarbital. Arch Biochem Biophys. 2001 Aug 1;392(1):59-64. doi: 10.1006/abbi.2001.2437. PMID: 11469794.


4: Collins AM, Blanchard GJ, Hawkett J, Collison D, Marken F. Liquid|liquid|electrode triple-phase boundary photovoltammetry of pentoxyresorufin in 4-(3-phenylpropyl)pyridine. Langmuir. 2011 May 17;27(10):6471-7. doi: 10.1021/la2010584. Epub 2011 Apr 13. PMID: 21488643.


5: Lubet RA, Mayer RT, Cameron JW, Nims RW, Burke MD, Wolff T, Guengerich FP. Dealkylation of pentoxyresorufin: a rapid and sensitive assay for measuring induction of cytochrome(s) P-450 by phenobarbital and other xenobiotics in the rat. Arch Biochem Biophys. 1985 Apr;238(1):43-8. doi: 10.1016/0003-9861(85)90138-9. PMID: 3985627.


6: Rutten AA, Falke HE, Catsburg JF, Wortelboer HM, Blaauboer BJ, Doorn L, van Leeuwen FX, Theelen R, Rietjens IM. Interlaboratory comparison of microsomal ethoxyresorufin and pentoxyresorufin O-dealkylation determinations: standardization of assay conditions. Arch Toxicol. 1992;66(4):237-44. doi: 10.1007/BF02307168. PMID: 1514921.


7: Umegaki K, Saito K, Kubota Y, Sanada H, Yamada K, Shinozuka K. Ginkgo biloba extract markedly induces pentoxyresorufin O-dealkylase activity in rats. Jpn J Pharmacol. 2002 Dec;90(4):345-51. doi: 10.1254/jjp.90.345. PMID: 12501011.


8: Paolini M, Mesirca R, Pozzetti L, Sapone A, Cantelli-Forti G. Induction of CYP2B1 mediated pentoxyresorufin O-dealkylase activity in different species, sex and tissue by prototype 2B1-inducers. Chem Biol Interact. 1995 Mar 30;95(1-2):127-39. doi: 10.1016/0009-2797(94)03352-8. PMID: 7697745.


9: Rastogi S, Das M, Khanna SK. A novel approach to study the activity and stoichiometry simultaneously for microsomal pentoxyresorufin-O-dealkylase reaction. FEBS Lett. 2002 Feb 13;512(1-3):121-4. doi: 10.1016/s0014-5793(02)02234-2. PMID: 11852064.


10: Jeong TC, Chang HW, Lee ES, Jeon TW, Jeong HG, Holsapple MP. Skf 525-A induces cocaine N-demethylase, ethoxyresorufin O-deethylase, and pentoxyresorufin O-dealkylase activities by induction of cytochrome p-450 2B in female B6C3F1 mice. J Toxicol Environ Health A. 2004 Dec;67(23-24):1955-70. doi: 10.1080/15287390490514606. PMID: 15513895.


11: Park KS, Sohn DH, Veech RL, Song BJ. Pre-translational induction of pentoxyresorufin O-depentylase by pyridine. Biochem Biophys Res Commun. 1992 Jun 15;185(2):676-82. doi: 10.1016/0006-291x(92)91678-j. PMID: 1610360.


12: Müller D, Steinmetzer P, Pissowotzki K, Glöckner R. Induction of cytochrome P450 2B1-mRNA and pentoxyresorufin O-depentylation after exposure of precision- cut rat liver slices to phenobarbital. Toxicology. 2000 Apr 3;144(1-3):93-7. doi: 10.1016/s0300-483x(99)00194-8. PMID: 10781875.


13: Cai Y, Bennett D, Nair RV, Ceska O, Ashwood-Smith MJ, DiGiovanni J. Inhibition and inactivation of murine hepatic ethoxy- and pentoxyresorufin O-dealkylase by naturally occurring coumarins. Chem Res Toxicol. 1993 Nov- Dec;6(6):872-9. doi: 10.1021/tx00036a018. PMID: 8117927.


14: Nakajima T, Elovaara E, Park SS, Gelboin HV, Hietanen E, Vainio H. Monoclonal antibody-directed characterization of benzene, ethoxyresorufin and pentoxyresorufin metabolism in rat liver microsomes. Biochem Pharmacol. 1990 Sep 15;40(6):1255-61. doi: 10.1016/0006-2952(90)90391-w. PMID: 2403379.


15: Parmar D, Dhawan A, Seth PK. Evidence for O-dealkylation of 7-pentoxyresorufin by cytochrome P450 2B1/2B2 isoenzymes in brain. Mol Cell Biochem. 1998 Dec;189(1-2):201-5. doi: 10.1023/a:1006831724484. PMID: 9879672.


16: Siess MH, Pennec A, Gaydou E. Inhibition of ethoxy- and pentoxy-resorufin dealkylases of rat liver by flavones and flavonols: structure-activity relationship. Eur J Drug Metab Pharmacokinet. 1989 Jul-Sep;14(3):235-9. doi: 10.1007/BF03190104. PMID: 2612520.