WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H407344
CAS#: 927822-86-4
Description: KC7F2 is a potent HIF-1 pathway inhibitor with potential anticancer activity. KC7F2 is cytotoxic to a variety of cancer cell lines with an IC50 value of 15-25 µM. KC7F2 markedly inhibited HIF-mediated transcription in cells derived from different tumor types, including glioma, breast, and prostate cancers, and exhibited enhanced cytotoxicity under hypoxia. KC7F2 prevented the activation of HIF-target genes such as carbonic anhydrase IX, matrix metalloproteinase 2 (MMP2), endothelin 1, and enolase 1. KC7F2 worked through the down-regulation of HIF-1alpha protein synthesis, an effect accompanied by the suppression of the phosphorylation of eukaryotic translation initiation factor 4E binding protein 1 and p70 S6 kinase, key regulators of HIF-1alpha protein synthesis.
Hodoodo Cat#: H407344
Name: KC7F2
CAS#: 927822-86-4
Chemical Formula: C16H16Cl4N2O4S4
Exact Mass: 567.87
Molecular Weight: 570.354
Elemental Analysis: C, 33.69; H, 2.83; Cl, 24.86; N, 4.91; O, 11.22; S, 22.48
Synonym: KC7F2; KC 7F2; KC-7F2;
IUPAC/Chemical Name: N,N'-(disulfanediylbis(ethane-2,1-diyl))bis(2,5-dichlorobenzenesulfonamide)
InChi Key: REQLACDIZMLXIC-UHFFFAOYSA-N
InChi Code: InChI=1S/C16H16Cl4N2O4S4/c17-11-1-3-13(19)15(9-11)29(23,24)21-5-7-27-28-8-6-22-30(25,26)16-10-12(18)2-4-14(16)20/h1-4,9-10,21-22H,5-8H2
SMILES Code: ClC1=C(S(NCCSSCCNS(C2=C(Cl)C=CC(Cl)=C2)(=O)=O)(=O)=O)C=C(Cl)C=C1
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO, not in water
Shelf Life: >2 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info:
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| In vitro activity: | |
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The following data is based on the product molecular weight 570.35 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
| Formulation protocol: | |
| In vitro protocol: | |
| In vivo protocol: |
1: Huang X, He Z, Jiang X, Hou M, Tang Z, Zhen X, Liang Y, Ma J. Folic Acid Represses Hypoxia-Induced Inflammation in THP-1 Cells through Inhibition of the PI3K/Akt/HIF-1α Pathway. PLoS One. 2016 Mar 14;11(3):e0151553. doi: 10.1371/journal.pone.0151553. eCollection 2016. PubMed PMID: 26974319; PubMed Central PMCID: PMC4790958.
2: Cheng CC, Guan SS, Yang HJ, Chang CC, Luo TY, Chang J, Ho AS. Blocking heme oxygenase-1 by zinc protoporphyrin reduces tumor hypoxia-mediated VEGF release and inhibits tumor angiogenesis as a potential therapeutic agent against colorectal cancer. J Biomed Sci. 2016 Jan 28;23:18. doi: 10.1186/s12929-016-0219-6. PubMed PMID: 26822586; PubMed Central PMCID: PMC4730655.
3: Guan G, Zhang Y, Lu Y, Liu L, Shi D, Wen Y, Yang L, Ma Q, Liu T, Zhu X, Qiu X, Zhou Y. The HIF-1α/CXCR4 pathway supports hypoxia-induced metastasis of human osteosarcoma cells. Cancer Lett. 2015 Feb 1;357(1):254-64. doi: 10.1016/j.canlet.2014.11.034. Epub 2014 Nov 18. PubMed PMID: 25444927.
4: Li J, Jiang G, Chen Y, Chen L, Li Z, Wang Z, Wang X. Altered expression of hypoxia-Inducible factor-1α participates in the epileptogenesis in animal models. Synapse. 2014 Sep;68(9):402-9. doi: 10.1002/syn.21752. Epub 2014 Jun 17. PubMed PMID: 24889205.
5: Rathnasamy G, Ling EA, Kaur C. Hypoxia inducible factor-1α mediates iron uptake which induces inflammatory response in amoeboid microglial cells in developing periventricular white matter through MAP kinase pathway. Neuropharmacology. 2014 Feb;77:428-40. doi: 10.1016/j.neuropharm.2013.10.024. Epub 2013 Nov 1. PubMed PMID: 24184387.
6: Narita T, Yin S, Gelin CF, Moreno CS, Yepes M, Nicolaou KC, Van Meir EG. Identification of a novel small molecule HIF-1alpha translation inhibitor. Clin Cancer Res. 2009 Oct 1;15(19):6128-36. doi: 10.1158/1078-0432.CCR-08-3180. Epub 2009 Sep 29. PubMed PMID: 19789328; PubMed Central PMCID: PMC2770235.
7: Koh MY, Spivak-Kroizman TR, Powis G. Inhibiting the hypoxia response for cancer therapy: the new kid on the block. Clin Cancer Res. 2009 Oct 1;15(19):5945-6. doi: 10.1158/1078-0432.CCR-09-1650. Epub 2009 Sep 29. PubMed PMID: 19789327; PubMed Central PMCID: PMC2760048.
