WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H406768
CAS#: 68489-09-8
Description: WS-12 is a potent TRPM8 agonist that acts as a cooling agent (EC50 = 193 nM). WS-12 robustly activated TRPM8 in low micromolar concentrations (EC50 12+/-5 microM) thereby displaying a higher potency and efficacy compared to menthol (EC50 196+/-22 microM). The selectivity profile of WS-12, its several-fold higher potency and around two-fold increase in efficacy compared to menthol warrants its potential utility for therapy in chronic neuropathic pain states and as a diagnostic probe in prostate cancer.
Hodoodo Cat#: H406768
Name: WS-12
CAS#: 68489-09-8
Chemical Formula: C18H27NO2
Exact Mass: 289.20
Molecular Weight: 289.419
Elemental Analysis: C, 74.70; H, 9.40; N, 4.84; O, 11.06
Synonym: WS 12; WS-12; WS12; acoltremon
IUPAC/Chemical Name: (1R,2S)-2-isopropyl-N-(4-methoxyphenyl)-5-methylcyclohexane-1-carboxamide
InChi Key: HNSGVPAAXJJOPQ-ZMKYACIDSA-N
InChi Code: InChI=1S/C18H27NO2/c1-12(2)16-10-5-13(3)11-17(16)18(20)19-14-6-8-15(21-4)9-7-14/h6-9,12-13,16-17H,5,10-11H2,1-4H3,(H,19,20)/t13?,16-,17+/m0/s1
SMILES Code: O=C([C@H]1[C@H](C(C)C)CCC(C)C1)NC2=CC=C(OC)C=C2
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO, not in water
Shelf Life: >2 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info: Transient receptor potential melastatin-8 (TRPM8), a cationic ion channel is involved in detection of normal cooling-sensation in mammals. TRPM8 activation by cooling or chemical agonists have been shown to produce profound, mechanistically novel analgesia in chronic pain states such as neuropathic pain in rodents.
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The following data is based on the product molecular weight 289.42 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
---|---|---|---|
1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
Formulation protocol: | |
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In vivo protocol: |
1: Ma S, G G, Ak VE, Jf D, H H. Menthol derivative WS-12 selectively activates transient receptor potential melastatin-8 (TRPM8) ion channels. Pak J Pharm Sci. 2008 Oct;21(4):370-8. PubMed PMID: 18930858.