WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H130222
CAS#: 2376838-11-6
Description: sBADA is a FDAA probe (green fluorescent D-amino acid probe) that is used for labeling peptidoglycans in the cell wall of bacteria. sBADA demonstrated its utility for visualizing peptidoglycan synthesis in Gram-negative and Gram-positive bacterial species. The permeability of FDAAs toward the outer-membrane of Gram-negative organisms, pinpointing the probes available for effective labeling in these species. The fluorescent d-amino acids can serve as ideal PG probes to elucidate bacterial cell growth and division in high spatiotemporal resolution, or to simply label bacterial cell surfaces for a variety of other applications in diverse bacteria
Hodoodo Cat#: H130222
Name: sBADA
CAS#: 2376838-11-6
Chemical Formula: C17H21BF2N4O6S
Exact Mass: 458.12
Molecular Weight: 458.244
Elemental Analysis: C, 44.56; H, 4.62; B, 2.36; F, 8.29; N, 12.23; O, 20.95; S, 7.00
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Related CAS #: 2376838-11-6
Synonym: sBADA
IUPAC/Chemical Name: (R)-2-amino-3-(3-(5,5-difluoro-7,9-dimethyl-2-sulfo-5H-4λ4,5λ4-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-3-yl)propanamido)propanoic acid
InChi Key: OLMGTLBVHKBMSH-GFCCVEGCSA-N
InChi Code: InChI=1S/C17H21BF2N4O6S/c1-9-5-10(2)23-14(9)6-11-7-15(31(28,29)30)13(24(11)18(23,19)20)3-4-16(25)22-8-12(21)17(26)27/h5-7,12H,3-4,8,21H2,1-2H3,(H,22,25)(H,26,27)(H,28,29,30)/t12-/m1/s1
SMILES Code: CC1=C2N(C(C)=C1)B(F)([N]3=C(C(S(O)(=O)=O)=CC3=C2)CCC(NC[C@H](C(O)=O)N)=O)F
Appearance: To be determined
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: To be determined
Shelf Life: >2 years if stored properly
Drug Formulation: To be determined
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info: Fluorescent d-amino acids (FDAAs) enable efficient in situ labeling of peptidoglycan in diverse bacterial species. Conducted by enzymes involved in peptidoglycan biosynthesis, FDAA labeling allows specific probing of cell wall formation/remodeling activity, bacterial growth and cell morphology. Their broad application and high biocompatibility have made FDAAs an important and effective tool for studies of peptidoglycan synthesis and dynamics, which, in turn, has created a demand for the development of new FDAA probes
Biological target: | |
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The following data is based on the product molecular weight 458.24 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
---|---|---|---|
1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
Formulation protocol: | |
In vitro protocol: | |
In vivo protocol: |
1. Hsu YP, Rittichier J, Kuru E, Yablonowski J, Pasciak E, Tekkam S, Hall E, Murphy B, Lee TK, Garner EC, Huang KC, Brun YV, VanNieuwenhze MS. Full color palette of fluorescent d-amino acids for in situ labeling of bacterial cell walls. Chem Sci. 2017 Sep 1;8(9):6313-6321. doi: 10.1039/c7sc01800b. Epub 2017 Jul 7. PMID: 28989665; PMCID: PMC5628581.