WST-8 sodium
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Hodoodo CAT#: H530578

CAS#: 193149-74-5 (sodium)

Description: WST-8 is a water-soluble tetrazolium salt used for assessing cell metabolic activity. At neutral pH and in the presence of the intermediate electron acceptor.

Chemical Structure

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WST-8 sodium
CAS# 193149-74-5 (sodium)
Instruction

Theoretical Analysis

Hodoodo Cat#: H530578
Name: WST-8 sodium
CAS#: 193149-74-5 (sodium)
Chemical Formula: C20H13N6NaO11S2
Exact Mass: 0.00
Molecular Weight: 600.465
Elemental Analysis: C, 40.01; H, 2.18; N, 14.00; Na, 3.83; O, 29.31; S, 10.68

Price and Availability

Size Price Availability Quantity
200mg USD 350 2 Weeks
500mg USD 750 2 Weeks
1g USD 1250 2 Weeks
2g USD 1950 2 Weeks
5g USD 3450 2 Weeks
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Related CAS #: 193149-74-5 (sodium)   755734-51-1 (free acid)    

Synonym: WST-8; WST 8; WST8.

IUPAC/Chemical Name: 4-(3-(2-methoxy-4-nitrophenyl)-2-(4-nitrophenyl)-2H-tetrazol-3-ium-5-yl)-3-sulfobenzenesulfonate, sodium salt

InChi Key: YCAKCISJXLQUEQ-UHFFFAOYSA-N

InChi Code: InChI=1S/C20H13N6O11S2.Na/c1-37-18-10-14(26(29)30)6-9-17(18)24-22-20(21-23(24)12-2-4-13(5-3-12)25(27)28)16-8-7-15(38(31,32)33)11-19(16)39(34,35)36;/h2-11H,1H3;/q-1;+1

SMILES Code: O=S(C1=CC=C(C2=NN(C3=CC=C([N+]([O-])=O)C=C3)[N+](C4=CC=C([N+]([O-])=O)C=C4OC)=N2)C([S-](=O)([O])=O)=C1)(O[Na])=O

Appearance: White to light yellow crystalline powder.

Purity: >98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.

Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).

Solubility: Soluble in DMSO

Shelf Life: >2 years if stored properly

Drug Formulation: This drug may be formulated in DMSO

Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).

HS Tariff Code: 2934.99.9001

More Info:

Biological target:
In vitro activity:
In vivo activity:

Preparing Stock Solutions

The following data is based on the product molecular weight 600.46 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Recalculate based on batch purity %
Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 1.15 mL 5.76 mL 11.51 mL
5 mM 0.23 mL 1.15 mL 2.3 mL
10 mM 0.12 mL 0.58 mL 1.15 mL
50 mM 0.02 mL 0.12 mL 0.23 mL
Formulation protocol:
In vitro protocol:
In vivo protocol:

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1: Tiwari K, Wavdhane M, Haque S, Govender T, Kruger HG, Mishra MK, Chandra R, Tiwari D. A sensitive WST-8-based bioassay for PEGylated granulocyte colony stimulating factor using the NFS-60 cell line. Pharm Biol. 2015 Jun;53(6):849-54. doi: 10.3109/13880209.2014.943248. Epub 2014 Nov 25. PubMed PMID: 25423580.

2: Stoddart MJ. WST-8 analysis of cell viability during osteogenesis of human mesenchymal stem cells. Methods Mol Biol. 2011;740:21-5. doi: 10.1007/978-1-61779-108-6_4. PubMed PMID: 21468964.

3: Tsukatani T, Suenaga H, Shiga M, Noguchi K, Ishiyama M, Ezoe T, Matsumoto K. Comparison of the WST-8 colorimetric method and the CLSI broth microdilution method for susceptibility testing against drug-resistant bacteria. J Microbiol Methods. 2012 Sep;90(3):160-6. doi: 10.1016/j.mimet.2012.05.001. Epub 2012 May 27. PubMed PMID: 22642794.

4: Brady AJ, Kearney P, Tunney MM. Comparative evaluation of 2,3-bis [2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT) and 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfophenyl)-2H-tetrazolium, monosodium salt (WST-8) rapid colorimetric assays for antimicrobial susceptibility testing of staphylococci and ESBL-producing clinical isolates. J Microbiol Methods. 2007 Dec;71(3):305-11. Epub 2007 Sep 29. PubMed PMID: 17963904.

5: Arai M, Kosuge K, Kawamoto F, Matsuoka H. Reactivity of blood samples spotted onto filter papers in the WST-8 method for screening of G6PD deficiency. Acta Med Okayama. 2006 Apr;60(2):127-34. PubMed PMID: 16680190.

6: Jung MY, Lee J, Park B, Hwang H, Sohn SO, Lee SH, Lim HI, Park HW, Lee JH. Applicability of a colorimetric method for evaluation of lactic acid bacteria with probiotic properties. Food Microbiol. 2017 Jun;64:33-38. doi: 10.1016/j.fm.2016.12.008. Epub 2016 Dec 18. PubMed PMID: 28213032.

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8: Tsukatani T, Suenaga H, Shiga M, Matsumoto K. A rapid microplate method for the proliferation assay of fungi and the antifungal susceptibility testing using the colorimetric microbial viability assay. Lett Appl Microbiol. 2014 Aug;59(2):184-92. doi: 10.1111/lam.12264. Epub 2014 May 5. PubMed PMID: 24713101.

9: Mohrmann C, Herold C, Pflaum M, Krämer R, Vogt PM, Allert S. [Viability and Particle Size of Fat Grafts Obtained with WAL and PAL Techniques]. Handchir Mikrochir Plast Chir. 2015 Aug;47(4):246-52. doi: 10.1055/s-0035-1547303. Epub 2015 Aug 19. German. PubMed PMID: 26287328.

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12: Tsukatani T, Suenaga H, Ishiyama M, Ezoe T, Matsumoto K. Determination of water-soluble vitamins using a colorimetric microbial viability assay based on the reduction of water-soluble tetrazolium salts. Food Chem. 2011 Jul 15;127(2):711-5. doi: 10.1016/j.foodchem.2010.12.130. Epub 2011 Jan 8. PubMed PMID: 23140724.

13: Tsukatani T, Suenaga H, Higuchi T, Shiga M, Noguchi K, Matsumoto K. Distinction of Gram-positive and -negative bacteria using a colorimetric microbial viability assay based on the reduction of water-soluble tetrazolium salts with a selection medium. J Gen Appl Microbiol. 2011;57(6):331-9. PubMed PMID: 22353738.

14: Tsukatani T, Higuchi T, Suenaga H, Akao T, Ishiyama M, Ezoe T, Matsumoto K. Colorimetric microbial viability assay based on reduction of water-soluble tetrazolium salts for antimicrobial susceptibility testing and screening of antimicrobial substances. Anal Biochem. 2009 Oct 1;393(1):117-25. doi: 10.1016/j.ab.2009.06.026. Epub 2009 Jun 26. PubMed PMID: 19560434.

15: Nakatani Y, Tsuji M, Amano T, Miyagawa K, Miyagishi H, Saito A, Imai T, Takeda K, Ishii D, Takeda H. Neuroprotective effect of yokukansan against cytotoxicity induced by corticosterone on mouse hippocampal neurons. Phytomedicine. 2014 Sep 25;21(11):1458-65. doi: 10.1016/j.phymed.2014.06.004. Epub 2014 Jul 8. PubMed PMID: 25022209.

16: Wang XQ, Yao RQ, Liu X, Huang JJ, Qi DS, Yang LH. Quercetin protects oligodendrocyte precursor cells from oxygen/glucose deprivation injury in vitro via the activation of the PI3K/Akt signaling pathway. Brain Res Bull. 2011 Oct 10;86(3-4):277-84. doi: 10.1016/j.brainresbull.2011.07.014. Epub 2011 Jul 23. PubMed PMID: 21803128.

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18: Lindl T, Lewandowski B, Schreyögg S, Stäudte A. An evaluation of the in vitro cytotoxicities of 50 chemicals by using an electrical current exclusion method versus the neutral red uptake and MTT assays. Altern Lab Anim. 2005 Dec;33(6):591-601. PubMed PMID: 16372834.

19: Walters NJ, Xia W, Salih V, Ashley PF, Young AM. Poly(propylene glycol) and urethane dimethacrylates improve conversion of dental composites and reveal complexity of cytocompatibility testing. Dent Mater. 2016 Feb;32(2):264-77. doi: 10.1016/j.dental.2015.11.017. Epub 2016 Jan 4. PubMed PMID: 26764174.

20: Latiff NM, Teo WZ, Sofer Z, Fisher AC, Pumera M. The Cytotoxicity of Layered Black Phosphorus. Chemistry. 2015 Sep 28;21(40):13991-5. doi: 10.1002/chem.201502006. Epub 2015 Aug 20. PubMed PMID: 26291565.