WARNING: This product is for research use only, not for human or veterinary use.
Hodoodo CAT#: H462695
CAS#: 9005-65-6
Description: Polysorbate 80 is a nonionic surfactant and emulsifier often used in foods and cosmetics. This synthetic compound is a viscous, water-soluble yellow liquid.
Hodoodo Cat#: H462695
Name: Polysorbate 80
CAS#: 9005-65-6
Chemical Formula: C32H60O10
Exact Mass: 604.42
Molecular Weight: 604.822
Elemental Analysis: C, 63.55; H, 10.00; O, 26.45
Synonym: Tween 80; Polysorbate 80;
IUPAC/Chemical Name: 2-(2-(3,5-bis(2-hydroxyethoxy)tetrahydrofuran-2-yl)-2-(2-hydroxyethoxy)ethoxy)ethyl (E)-octadec-9-enoate
InChi Key: RGPBUVUVZKQNHD-MDZDMXLPSA-N
InChi Code: InChI=1S/C32H60O10/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-30(36)40-25-24-37-27-29(39-22-19-34)32-28(38-21-18-33)26-31(42-32)41-23-20-35/h9-10,28-29,31-35H,2-8,11-27H2,1H3/b10-9+
SMILES Code: CCCCCCCC/C=C/CCCCCCCC(OCCOCC(OCCO)C1C(OCCO)CC(OCCO)O1)=O
Appearance: Solid powder
Purity: >98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical. This product is stable enough for a few weeks during ordinary shipping and time spent in Customs.
Storage Condition: Dry, dark and at 0 - 4 C for short term (days to weeks) or -20 C for long term (months to years).
Solubility: Soluble in DMSO
Shelf Life: >3 years if stored properly
Drug Formulation: This drug may be formulated in DMSO
Stock Solution Storage: 0 - 4 C for short term (days to weeks), or -20 C for long term (months).
HS Tariff Code: 2934.99.9001
More Info:
| Biological target: | |
| In vitro activity: | |
| In vivo activity: |
The following data is based on the product molecular weight 604.82 Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.15 mL | 5.76 mL | 11.51 mL |
| 5 mM | 0.23 mL | 1.15 mL | 2.3 mL |
| 10 mM | 0.12 mL | 0.58 mL | 1.15 mL |
| 50 mM | 0.02 mL | 0.12 mL | 0.23 mL |
| Formulation protocol: | |
| In vitro protocol: | |
| In vivo protocol: |
1: Schwartzberg LS, Navari RM. Safety of Polysorbate 80 in the Oncology Setting. Adv Ther. 2018 Jun;35(6):754-767. doi: 10.1007/s12325-018-0707-z. Epub 2018 May 23. PMID: 29796927; PMCID: PMC6015121.
2: Palacios Castaño MI, Venturini Díaz M, Lobera Labairu T, González Mahave I, Del Pozo Gil MD, Blasco Sarramián A. Anaphylaxis Due to the Excipient Polysorbate 80. J Investig Allergol Clin Immunol. 2016;26(6):394-396. doi: 10.18176/jiaci.0109. PMID: 27996954.
3: Kerwin BA. Polysorbates 20 and 80 used in the formulation of protein biotherapeutics: structure and degradation pathways. J Pharm Sci. 2008 Aug;97(8):2924-35. doi: 10.1002/jps.21190. PMID: 17973307.
4: Gao M, Mei D, Huo Y, Mao S. Effect of polysorbate 80 on the intranasal absorption and brain distribution of tetramethylpyrazine phosphate in rats. Drug Deliv Transl Res. 2019 Feb;9(1):311-318. doi: 10.1007/s13346-018-0580-y. PMID: 30168052.
5: Wagner N, Podda M. High volume of polysorbate-containing (Tween® 80) solutions induces false-positive results in intradermal test. J Eur Acad Dermatol Venereol. 2018 Nov;32(11):1972-1976. doi: 10.1111/jdv.14999. Epub 2018 Jun 13. PMID: 29633392.
6: Zdravkovic SA. Comparison of the Extraction Properties of Binary Polysorbate 80/Water and Isopropanol/Water Solutions for Organic Substances Originating from a Parenteral Infusion Pump. PDA J Pharm Sci Technol. 2019 Nov-Dec;73(6):588-614. doi: 10.5731/pdajpst.2018.009753. Epub 2019 Aug 16. PMID: 31420503.
7: Dwivedi M, Blech M, Presser I, Garidel P. Polysorbate degradation in biotherapeutic formulations: Identification and discussion of current root causes. Int J Pharm. 2018 Dec 1;552(1-2):422-436. doi: 10.1016/j.ijpharm.2018.10.008. Epub 2018 Oct 6. PMID: 30300706.
8: Gopalrathnam G, Sharma AN, Dodd SW, Huang L. Impact of Stainless Steel Exposure on the Oxidation of Polysorbate 80 in Histidine Placebo and Active Monoclonal Antibody Formulation. PDA J Pharm Sci Technol. 2018 Mar- Apr;72(2):163-175. doi: 10.5731/pdajpst.2017.008284. Epub 2018 Jan 17. PMID: 29343621.
9: Malinowski AM, McClarty BM, Robinson C, Spear W, Sanchez M, Sparkes TC, Brooke JS. Polysorbate 80 and polymyxin B inhibit Stenotrophomonas maltophilia biofilm. Diagn Microbiol Infect Dis. 2017 Feb;87(2):154-156. doi: 10.1016/j.diagmicrobio.2016.11.008. Epub 2016 Nov 14. PMID: 27923523.
10: Singh SR, Zhang J, O'Dell C, Hsieh MC, Goldstein J, Liu J, Srivastava A. Effect of polysorbate 80 quality on photostability of a monoclonal antibody. AAPS PharmSciTech. 2012 Jun;13(2):422-30. doi: 10.1208/s12249-012-9759-6. Epub 2012 Feb 24. PMID: 22362139; PMCID: PMC3364395.
11: Figura N, Marcolongo R, Cavallo G, Santucci A, Collodel G, Spreafico A, Moretti E. Polysorbate 80 and Helicobacter pylori: a microbiological and ultrastructural study. BMC Microbiol. 2012 Sep 22;12:217. doi: 10.1186/1471-2180-12-217. PMID: 22998649; PMCID: PMC3537525.
12: Jäpelt KB, Johnsen LG, Christensen JH. Removal of Polysorbate 80 by complexation prior to LC-MS analysis. Anal Bioanal Chem. 2016 Mar;408(9):2303-7. doi: 10.1007/s00216-016-9326-1. Epub 2016 Feb 19. PMID: 26894763.
13: Nguyen NT. Acinetobacter soli SP2 Capable of High-Efficiency Degradation of Food Emulsifier Polysorbate 80. Curr Microbiol. 2018 Jul;75(7):896-900. doi: 10.1007/s00284-018-1463-7. Epub 2018 Feb 24. PMID: 29478241.
14: Puschmann J, Evers DH, Müller-Goymann CC, Herbig ME. Development of a design of experiments optimized method for quantification of polysorbate 80 based on oleic acid using UHPLC-MS. J Chromatogr A. 2019 Aug 16;1599:136-143. doi: 10.1016/j.chroma.2019.04.015. Epub 2019 Apr 8. PMID: 31003714.
15: Agarkhed M, O'Dell C, Hsieh MC, Zhang J, Goldstein J, Srivastava A. Effect of polysorbate 80 concentration on thermal and photostability of a monoclonal antibody. AAPS PharmSciTech. 2013 Mar;14(1):1-9. doi: 10.1208/s12249-012-9878-0. Epub 2012 Nov 14. PMID: 23152309; PMCID: PMC3581664.
16: Zhang Q, Wang A, Meng Y, Ning T, Yang H, Ding L, Xiao X, Li X. NMR method for accurate quantification of polysorbate 80 copolymer composition. Anal Chem. 2015 Oct 6;87(19):9810-6. doi: 10.1021/acs.analchem.5b02096. PMID: 26356097.
17: Wei H, Liu T, Jiang N, Zhou K, Yang K, Ning W, Yu Y. A Novel Delivery System of Cyclovirobuxine D for Brain Targeting: Angiopep-Conjugated Polysorbate 80-Coated Liposomes via Intranasal Administration. J Biomed Nanotechnol. 2018 Jul 1;14(7):1252-1262. doi: 10.1166/jbn.2018.2581. PMID: 29944099.
18: Ganguly R, Kumar S, Nath S, Aswal VK. Salt and phytochemical assisted modulation of self-assembly characteristics of Polysorbate-80 in aqueous medium. Int J Pharm. 2019 May 30;563:63-70. doi: 10.1016/j.ijpharm.2019.03.055. Epub 2019 Mar 29. PMID: 30935912.
19: Damico FM, Gasparin F, Ioshimoto GL, Igami TZ, Cunha ADS Jr, Fialho SL, Liber AM, Young LH, Ventura DF. Intravitreal injection of polysorbate 80: a functional and morphological study. Rev Col Bras Cir. 2017 Nov- Dec;44(6):603-611. English, Portuguese. doi: 10.1590/0100-69912017006010. PMID: 29267557.
20: Singh SM, Bandi S, Jones DNM, Mallela KMG. Effect of Polysorbate 20 and Polysorbate 80 on the Higher-Order Structure of a Monoclonal Antibody and Its Fab and Fc Fragments Probed Using 2D Nuclear Magnetic Resonance Spectroscopy. J Pharm Sci. 2017 Dec;106(12):3486-3498. doi: 10.1016/j.xphs.2017.08.011. Epub 2017 Aug 24. PMID: 28843351; PMCID: PMC6178931.
